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Molecular genetics alterations and tumor behavior of sporadic vestibular schwannoma from the People’s Republic of China

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Abstract

Objectives: To analyze the molecular genetic alteration of sporadic vestibular schwannomas from the People’s Republic of China and to correlate these alterations with the tumor behaviors. Methods: Four highly polymorphic microsatellite DNA markers were used to observe the frequency of loss of heterozygosity (LOH) in chromosome 22. The NF2 gene mutations were detected by Polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) and DNA sequencing. The schwannomin/merlin (S/M) expression was examined using anti-NF2 (A-19) IgG under immunohistochemistry and western blot. The proliferative index (LI) of vestibular schwannoma was evaluated by proliferative cell nuclear antigen investigation. Results: Sixteen vestibular schwannomas (44.4%) showed allele loss. We found 22 mutations in 36 schwannomas. The LI and the growth rate of schwannomas with LOH or mutation were significantly higher than those without LOH or mutation. All of these vestibular schwannomas showed no immunoreaction to anti-NF2(A-19) IgG by immunohistochemistry. By immunoblotting technique, reduced expression of S/M was found in 31 cases (86%). The growth index of schwannomas with severely reduced expression of S/M was significantly higher than those with moderately reduced or normal expression. Conclusion: The molecular genetic changes in sporadic vestibular schwannomas from Chinese patients were similar to the previous reports. We demonstrate the relationship between tumor behaviors and genetic alteration (including LOH and mutation of NF2 gene). We propose that inactivation of S/M, may be an important step in tumorigenesis of sporadic vestibular schwannoma.

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Authors and Affiliations

  1. Department of Neurosurgery, Rui-Jin Hospital, Shanghai Second Medical University, Shanghai, The People’s Republic of China

    Liu-Guan Bian, Qing-Fang Sun, Wei-Guo Zhao & Jian-Kang Shen

  2. Department of Neurosurgery, Philipps University, Marburg, Germany

    Liu-Guan Bian & Wuttipong Tirakotai

  3. Department of Neurosurgery, Ren-Ji Hospital, Shanghai Second Medical University, Shanghai

    Qi-Zhong Luo

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  1. Liu-Guan Bian
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  2. Wuttipong Tirakotai
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Correspondence to Liu-Guan Bian.

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Bian, LG., Tirakotai, W., Sun, QF. et al. Molecular genetics alterations and tumor behavior of sporadic vestibular schwannoma from the People’s Republic of China. J Neurooncol 73, 253–260 (2005). https://doi.org/10.1007/s11060-004-5176-3

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