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Fire-related cues improve germination and seedling vigor of the post-fire off-spring of Pinus radiata, a serotinous invader tree

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Abstract

Germination rate and seedling establishment control post-fire regeneration of trees and may foster invasion of exotic species particularly of fire-adapted species as the serotinous Pinus radiata. We analyzed the influence of heat shock and the presence of ash as substrate on seed germination and early vigor of seedlings of distinct cohorts of P. radiata that developed under the influence of fire or in the absence of it, i.e. post-fire invasion and unburned plantation, respectively. Seeds were collected from the Reserva Forestal Lago Epuyén in Northern Patagonia, Argentina. The natural history of this region is characterized by the occurrence of extensive fires. Ten randomly selected trees from unburned plantations and post-fire invasion that occurred in 1987 of similar age were cut down and 10 serotinous cones by plant were collected without color or size bias. Seeds from both stand types were subjected to four treatments: ash solution (5 g/L) and two thermal shocks (90 and 120 °C for 5 min), and a control without thermal shock or ash presence. The number of germinated seeds was counted every 5 days during 30 days. Germination percentages observed were high (between 80 and 100%) for both seed sources and under all the fire-associated treatments. Post-fire invasion had longer radicles with ash and under the thermal shock at 90 °C, as well as a higher percentage of seedlings that developed cotyledons and with the highest survival. Recurrent fires exert a significant effect on germination and early growth of the exotic P. radiata which may foster invasion.

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References

  • Alanís Rodríguez E, Jiménez Pérez J, González Tagle MA et al (2014) Effects of fires on the structure of the undergrowth in a temperate ecosystem. Rev Mex Ciencias for 5:74–85

    Google Scholar 

  • Álvarez R, Valbuena L, Calvo L (2007) Effect of high temperatures on seed germination and seedling survival in three pine species (Pinus pinaster, P. sylvestris and P. nigra). Int J Wildl Fire 16:63–70

  • Arán D, García-Duro J, Reyes O, Casal M (2013) Fire and invasive species: modifications in the germination potential of Acacia melanoxylon, Conyza canadensis and Eucalyptus globulus. For Ecol Manag 302:7–13. https://doi.org/10.1016/j.foreco.2013.02.030

  • Blackhall M, Franzese J, Raffaele E, Gobbi ME (2016) Efecto del fuego sobre la germinación de especies leñosas y trepadoras de los bosques y matorrales del noroeste patagónico In: III Jornadas Forestales de Patagonia Sur y Ecofuego II Esquel

  • Bond WJ, van Wilgen BW (2012) Fire and plants. Springer, New York

    Google Scholar 

  • Bretz F, Hothorn T, Westfall P (2016) Multiple comparisons using R. CRC Press, Florida

    Book  Google Scholar 

  • Broncano MJ, Riba M, Retana J (1998) Seed germination and seedling performance of two Mediterranean tree species, holm oak (Quercus ilex L.) and Aleppo pine (Pinus halepensis Mill.): a multifactor experimental approach. Plant Ecol 138:17–26

  • Bustamante RO, Serey IA, Pickett STA (2003) Forest fragmentation, plant regeneration and invasion processes across edges in central chile. In: Bradshaw GA, Marquet PA (eds) How landscapes change. Ecological studies, vol 162. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-05238-9_9

  • Callejas-Díaz M, Chambel MR, San-Martín-Lorén J et al (2022) The role of maternal age, growth, and environment in shaping offspring performance in an aerial conifer seed bank. Am J Bot 109:366–376

    Article  PubMed  PubMed Central  Google Scholar 

  • Calviño-Cancela M, Lorenzo P, González L (2018) Fire increases Eucalyptus globulus seedling recruitment in forested habitats: effects of litter, shade and burnt soil on seedling emergence and survival. For Ecol Manag 409:826–834

  • Çatav ŞS, Küçükakyüz K, Tavşanoğlu Ç, Pausas JG (2018) Effect of fire-derived chemicals on germination and seedling growth in Mediterranean plant species. Basic Appl Ecol 30:65–75. https://doi.org/10.1016/j.baae.2018.05.005

    Article  Google Scholar 

  • Cóbar-Carranza AJ, Garcia RA, Pauchard A, Pena E (2015) Effects of high temperatures in the germination and seed survival of the invasive species Pinus contorta and two native species of South Chile. Bosque 36:53–60

  • Cruz O, García-Duro J, Casal M, Reyes O (2019) Role of serotiny on Pinus pinaster aiton germination and its relation to mother plant age and fire severity. Iforest 12:491–497. https://doi.org/10.3832/ifor2968-012

  • Cuello N, López-Mársico L, Rodríguez C (2020) Field burn versus fire-related cues: germination from the soil seed bank of a South American temperate grassland. Seed Sci Res 30:206–214. https://doi.org/10.1017/S0960258520000288

    Article  CAS  Google Scholar 

  • D’Antonio CM, Vitousek PM (1992) Biological invasions by exotic grasses, the grass/fire cycle, and global change. Annu Rev Ecol Syst 23:63–87. https://doi.org/10.1146/annurev.es.23.110192.000431

    Article  Google Scholar 

  • Fontenele HGV, Cruz-Lima LFS, Pacheco-Filho JL, Miranda HS (2020) Burning grasses, poor seeds: post-fire reproduction of early-flowering Neotropical savanna grasses produces low-quality seeds. Plant Ecol 221:1265–1274. https://doi.org/10.1007/s11258-020-01080-7

    Article  Google Scholar 

  • Forbes C, Evans M, Hastings N, Peacock B (2011) Statistical distributions. John Wiley & Sons

  • Franzese J, Ghermandi L (2014) Seed longevity and fire: post-germination responses of Rumex acetosella L. in northwest Patagonian grasslands (Argentina). Plant Species Biol 29:202–206. https://doi.org/10.1111/1442-1984.12012

  • Franzese J, Raffaele E (2017) Fire as a driver of pine invasions in the Southern Hemisphere: a review. Biol Invasions 19:2237–2246. https://doi.org/10.1007/s10530-017-1435-z

    Article  Google Scholar 

  • Furtado Fernandes A, Oki Y, Wilson Fernandes G, Moreira B (2021) The effect of fire on seed germination of campo rupestre species in the South American Cerrado. Plant Ecol 222:45–55. https://doi.org/10.1007/s11258-020-01086-1

    Article  Google Scholar 

  • Goubitz S, Werger MJA, Ne’eman G (2003) Germination response to fire-related factors of seeds from non-serotinous and serotinous cones. Plant Ecol 169:195–204. https://doi.org/10.1023/A:1026036332277

    Article  Google Scholar 

  • Grime JP (2006) Plant strategies, vegetation processes, and ecosystem properties. Wiley

  • Guthrie SG, Crandall RM, Knight TM (2016) Fire indirectly benefits fitness in two invasive species. Biol Invasions 18:1265–1273

    Article  Google Scholar 

  • Harper JL (1977) Population biology of plants. Academic Press

  • Haslem A, Leonard SWJ, Bruce MJ et al (2016) Do multiple fires interact to affect vegetation structure in temperate eucalypt forests? Ecol Appl 26:2414–2423. https://doi.org/10.1002/eap.1399

    Article  Google Scholar 

  • He T, Lamont BB, Pausas JG (2019) Fire as a key driver of Earth’s biodiversity. Biol Rev 94:1983–2010. https://doi.org/10.1111/brv.12544

    Article  PubMed  Google Scholar 

  • Herrero C, San Martin R, Bravo F (2007) Effect of heat and ash treatments on germination of Pinus pinaster and Cistus laurifolius. J Arid Environ 70:540–548. https://doi.org/10.1016/j.jaridenv.2006.12.027

  • Higgins SI, Richardson DM (1998) Pine invasions in the southern hemisphere: modelling interactions between organism, environment and disturbance. Plant Ecol 135:79–93

    Article  Google Scholar 

  • Hille M, den Ouden J (2004) Improved recruitment and early growth of Scots pine (Pinus sylvestris L.) seedlings after fire and soil scarification. Eur J for Res 123:213–218. https://doi.org/10.1007/s10342-004-0036-4

  • Hodges JA, Price JN, Nimmo DG, Guja LK (2019) Evidence for direct effects of fire-cues on germination of some perennial forbs common in grassy ecosystems. Austral Ecol 44:1271–1284. https://doi.org/10.1111/aec.12806

    Article  Google Scholar 

  • Keeley JE, Pausas JG, Rundel PW et al (2011) Fire as an evolutionary pressure shaping plant traits. Trends Plant Sci 16:406–411. https://doi.org/10.1016/j.tplants.2011.04.002

    Article  CAS  PubMed  Google Scholar 

  • Keeley JE, Fotheringham CJ (2000) Role of fire in regeneration from seed In: Seeds: the ecology of regeneration in plant communities Cab International 2nd. edn. Oxon, pp 311–330

  • Kerns BK, Day MA (2018) Prescribed fire regimes subtly alter ponderosa pine forest plant community structure. Ecosphere 9:e02529. https://doi.org/10.1002/ecs2.2529

    Article  Google Scholar 

  • Kitzberger T (1994) Fire regime variation along a northern Patagonian forest-steppe ecotone: stand and landscape response Ph D thesis, Dep Geogr Univ Color Boulder, Color

  • Lamont BB, Enright NJ (2000) Adaptive advantages of aerial seed banks. Plant Species Biol 15:157–166. https://doi.org/10.1046/j.1442-1984.2000.00036.x

    Article  Google Scholar 

  • Lamont BB, He T (2017) Fire-proneness as a prerequisite for the evolution of fire-adapted traits. Trends Plant Sci 22:278–288. https://doi.org/10.1016/j.tplants.2016.11.004

    Article  CAS  PubMed  Google Scholar 

  • Lamont BB, Le Maitre DC, Cowling RM, Enright NJ (1991) Canopy seed storage in woody plants. Bot Rev 57:277–317. https://doi.org/10.1007/BF02858770

    Article  Google Scholar 

  • Lamont BB, Pausas JG, He T et al (2020) Fire as a selective agent for both serotiny and nonserotiny over space and time. CRC Crit Rev Plant Sci 39:140–172. https://doi.org/10.1080/07352689.2020.1768465

    Article  CAS  Google Scholar 

  • MacDougall AS, McCann KS, Gellner G, Turkington R (2013) Diversity loss with persistent human disturbance increases vulnerability to ecosystem collapse. Nature 494:86–89

    Article  CAS  PubMed  Google Scholar 

  • Manfredi R, Postler V, Urretavizcaya F, et al (1999) Plan Estratégico de Manejo de la Reserva Forestal Cuartel Lago Epuyén. Caracter y Diagnóstico Cons Fed Inversiones, Dir Gen Bosques y Parques la Prov del Chubut, Cent Investig y Extensión For Andin Patagónico 162

  • McDonald PM, Laacke RJ (1990) Pinus radiata D. Don for Serv Agric Handb 1:433–441

  • McLauchlan KK, Higuera PE, Miesel J et al (2020) Fire as a fundamental ecological process: research advances and frontiers. J Ecol 108:2047–2069. https://doi.org/10.1111/1365-2745.13403

    Article  Google Scholar 

  • Mead DJ (2013) Sustainable management of Pinus radiata plantations. FAO Forest, Rome

  • Murphy GEP, Romanuk TN (2014) A meta-analysis of declines in local species richness from human disturbances. Ecol Evol 4:91–103

    Article  PubMed  Google Scholar 

  • Naghipour AA, Bashari H, Khajeddin SJ et al (2016) Effects of smoke, ash and heat shock on seed germination of seven species from Central Zagros rangelands in the semi-arid region of Iran. African J Range Forage Sci 33:67–71. https://doi.org/10.2989/10220119.2015.1119194

    Article  Google Scholar 

  • Nuñez MA, Raffaele E (2007) Afforestation causes changes in post-fire regeneration in native shrubland communities of northwestern Patagonia, Argentina. J Veg Sci 18:827–834

    Article  Google Scholar 

  • Olguin Loreto Labbe D (2013) Efecto del fuego sobre la viabilidad y germinación de semillas en ecosistemas Patagónicos. Fac Ciencias For y Recur Nat Univ Austral Chile, Thesis

    Google Scholar 

  • Ooi MKJ, Denham AJ, Santana VM, Auld TD (2014) Temperature thresholds of physically dormant seeds and plant functional response to fire: variation among species and relative impact of climate change. Ecol Evol 4:656–671

    Article  PubMed  PubMed Central  Google Scholar 

  • Pausas JG, Keeley JE (2014) Evolutionary ecology of resprouting and seeding in fire-prone ecosystems. New Phytol 204:55–65. https://doi.org/10.1111/nph.12921

    Article  PubMed  Google Scholar 

  • Perkins JL (2015) Fire enhances whitebark pine seedling establishment, survival, and growth. Fire Ecol 11:84–99. https://doi.org/10.4996/fireecology.1102084

    Article  Google Scholar 

  • Premoli AC (1996) Leaf architecture of South American Nothofagus (Nothofagaceae) using traditional and new methods in morphometrics. Bot J Linn Soc 121:25–40

    Google Scholar 

  • Raffaele E, Nuñez MA, Eneström J, Blackhall M (2016) Fire as mediator of pine invasion: evidence from Patagonia, Argentina. Biol Invasions 18:597–601. https://doi.org/10.1007/s10530-015-1038-5

    Article  Google Scholar 

  • R CoreTeam (2018) R: a language and environment for statistical computing

  • Reyes O, Casal M (1998) Germination of Pinus pinaster, P. radiata and Eucalyptus globulus in relation to the amount of ash produced in forest fires. Ann Des Sci for 55:837–845. https://doi.org/10.1051/forest:19980707

  • Reyes O, Casal M (2001) The influence of seed age on germinative response to the effects of fire in Pinus pinaster, Pinus radiata and Eucalyptus globulus. Ann for Sci 58:439–447

  • Reyes O, Casal M (2004) Effects of forest fire ash on germination and early growth of four pinus species. Plant Ecol 175:81–89. https://doi.org/10.1023/B:VEGE.0000048089.25497.0c

    Article  Google Scholar 

  • Reyes O, García-Duro J, Salgado J (2015a) Fire affects soil organic matter and the emergence of Pinus radiata seedlings. Ann for Sci 72:267–275

  • Reyes O, Kaal J, Arán D et al (2015b) The effects of ash and black carbon (biochar) on germination of different tree species. Fire Ecol 11:119–133

    Article  Google Scholar 

  • Reyes O, Casal M (1995) Germination behaviour of 3 species of the genus Pinus in relation to high temperatures suffered during forest fires. In: Annales des sciences forestières EDP Sciences, pp 385–392

  • Reyes O, Casal M (2002) Role of fire on seed dissemination and germination of Pinus pinaster and P. radiata. Nova Acta Científica Compostelana (Bioloxía), 12:111–118

  • Richardson DM, Rundel PW (1998) Ecology and Biogeography of Pinus: An Introduction. In: Richardson DM ed Ecology and Biogeography of Pinus, Cambridge University Press, Cambridge, pp. 3–46

  • Richardson DM, Brown PJ (1986) Invasion of mesic mountain fynbos by Pinus radiata. South African J Bot 52:529–536

  • Ripa RR, Franzese J, Premoli AC, Raffaele E (2020) Increased canopy seed-storage in post-fire pine invaders suggests rapid selection mediated by fire. Basic Appl Ecol 45:76–85. https://doi.org/10.1016/j.baae.2020.04.002

    Article  Google Scholar 

  • Ripa RR (2021) El fuego como agente de selección rápida en la invasión de la conífera exótica Pinus radiata: un análisis ecológico-molecular Ph D Thesis, Cent Reg Univ Bariloche, Univ Nac del Comahue

  • Rogers DL (2004) In situ genetic conservation of a naturally restricted and commercially widespread species, Pinus radiata. For Ecol Manag 197:311–322. https://doi.org/10.1016/j.foreco.2004.05.022

  • Roy DF (1966) Silvical characteristics of Monterey Pine (Pinus radiata). US for Serv Res Pap PSW - 31:1–21

  • Silva JS, dos Santos P, Sério A, Gomes F (2016) Effects of heat on dehiscence and germination in Eucalyptus globulus Labill. Int J Wildl Fire 25:478. https://doi.org/10.1071/WF15078

  • Stephens SL, Piirto DD, Caramagno DF (2004) Fire regimes and resultant forest structure in the Native Año Nuevo Monterey Pine (Pinus radiata) Forest, California. Am Midl Nat 152:25–36. https://doi.org/10.1674/0003-0031(2004)152[0025:frarfs]2.0.co;2

  • Tilman D (1990) Constraints and tradeoffs: toward a predictive theory of competition and succession Oikos 3–15

  • Todorović S, Božić D, Simonović A et al (2010) Interaction of fire-related cues in seed germination of the potentially invasive species Paulownia tomentosa Steud. Plant Species Biol 25:193–202. https://doi.org/10.1111/j.1442-1984.2010.00293.x

  • Urretavizcaya MF, Defossé GE (2004) Soil seed bank of Austrocedrus chilensis (D. Don) Pic. Serm. et Bizarri related to different degrees of fire disturbance in two sites of southern Patagonia. Argentina for Ecol Manag 187:361–372. https://doi.org/10.1016/j.foreco.2003.07.001

  • Veblen TT, Lorenz DC (1987) Post-fire stand development of Austrocedrus-Nothofagus forests in northern Patagonia. Vegetatio 71:113–126

    Article  Google Scholar 

  • Veblen TT, Donoso C, Kitzberger T, Rebertus AJ (1996) Ecology of southern Chilean and Argentinean Nothofagus forests. Ecol Biogeogr Nothofagus for 10:93–353

    Google Scholar 

  • Veblen TT, Holz A, Paritsis J et al (2011) Adapting to global environmental change in Patagonia: what role for disturbance ecology? Austral Ecol 36:891–903

    Article  Google Scholar 

  • Willan RL (1986) A guide to forest seed handling with special reference to the tropics FAO For Pap

  • Williams MC, Wardle GM (2005) The invasion of two native Eucalypt forests by Pinus radiata in the Blue Mountains, New South Wales, Australia. Biol Conserv 125:55–64

  • Woodard PM (1983) Germination success of Pinus contorta Dougl. and Picea Engelmannii parry on burned seedbeds. For Ecol Manag 5:301–306. https://doi.org/10.1016/0378-1127(83)90034-8

  • Zuluaga MS, Acciaresi HA, Chidichimo HO (2004) Comparación de la viabilidad de las semillas obtenidas por medio de las técnicas de extracción física por lavado y de germinación. Planta Daninha 22:225–229

    Article  Google Scholar 

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Acknowledgements

We thank Secretaria de Bosques de Chubut and Servicio de Prevención y Lucha Contra Incendios Forestales (SPLIF), and especially Gerardo Finster for providing us with valuable information about the natural history of the study site, for his logistical support and help with the selection of sampling sites. For their valuable help in the field we thank Ariel Mayoral and Pablo Alvear.

Funding

The study was funded by the Agencia Nacional de Promoción Científica y Tecnologica PICT 2014–3466 and PICT 2017–0396 and the Rufford Foundation Grant number 24894–1.

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  1. Grupo de Genética Ecológica, Evolutiva y de la Conservación, Instituto de Investigaciones en Biodiversidad y Medioambiente, Universidad Nacional del Comahue - CONICET, Quintral 1250, 8400, San Carlos de Bariloche, RN, Argentina

    Ramiro R. Ripa & Andrea C. Premoli

  2. Investigaciones de Ecología en Ambientes Antropizados, Laboratorio Ecotono, Instituto de Investigaciones en Biodiversidad y Medioambiente, Universidad Nacional del Comahue - CONICET, Quintral 1250, 8400, San Carlos de Bariloche, RN, Argentina

    Jorgelina Franzese & Estela Raffaele

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  1. Ramiro R. Ripa
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  4. Estela Raffaele
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Conceptualization: RR, JF, AP and ER; Methodology: RR and JF; Formal analysis and investigation: RR and JF; Writing—original draft preparation: RR; Writing—review and editing: RR, JF, AP and ER.

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Correspondence to Ramiro R. Ripa.

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Ripa, R.R., Franzese, J., Premoli, A.C. et al. Fire-related cues improve germination and seedling vigor of the post-fire off-spring of Pinus radiata, a serotinous invader tree. New Forests 54, 363–375 (2023). https://doi.org/10.1007/s11056-022-09928-8

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