A probabilistic model for tropical tree seed desiccation tolerance and storage classification
Knowledge of seed desiccation tolerance is fundamental for conservation and use of forest species. The protocol used for classification of seed desiccation tolerance and storage is time consuming and many times limited by the lack of information about optimum conditions for seed germination and treatments to overcome seed dormancy. This study evaluated 66 Brazilian tree species aiming to correlate seed characteristics with desiccation tolerance. For this purpose, a model was established to explain the relationship of tegument/seed mass ratio (SCR), seed mass, and water content of embryo + endosperm with desiccation tolerance. The principal component analysis showed the establishment of two groups, indicating the interaction between desiccation tolerance and seed characteristics. Recalcitrant seeds are more often associated with the water content of embryo + endosperm and water content of tegument + endocarp, while orthodox seeds are more associated with SCR and number of seeds per kilogram. The classification found using the model proposed was significantly correlated with desiccation tolerance and storage, with 92% confidence for the analyzed species. Seeds morphological characteristics can be used for prediction of desiccation tolerance and storage behavior; however, the use of a model that combines more variables increases the chance of accurate classification.
KeywordsSeed storage behavior Forest seeds Orthodox Recalcitrant
To the Coordination for the Improvement of Higher Education Personnel (CAPES), National Council for Scientific and Technological Development (CNPq), and Minas Gerais State Agency for Research and Development (FAPEMIG), geared to development support and execution of this research.
- Brasil, Ministério da Agricultura e da Reforma Agrária (2009) Regras para análise de sementes. Mapa/ACS, Brasília, p 399Google Scholar
- Costa CJ (2009) Armazenamento e conservação de sementes de espécies do cerrado. Planaltina, DF: Embrapa Cerrados, p. 30—(Documentos/Embrapa Cerrados, ISSN 1517-5111, ISSN online 2176-5081, 265)Google Scholar
- Gold K, Hay F (2008) Identifying desiccation-sensitive seeds. Technical Information Sheet. No 10. Millenium Seed Banks Project. Royal Botanical Gardens, Kew, UKGoogle Scholar
- Hong TD, Ellis RH (1996) A protocol to determine seed storage behavior. A protocol to determine seed storage behaviour. IPGRI Technical Bulletin No. 1. International Plant Genetic Resources Institute, Rome, ItalyGoogle Scholar
- Hong TD, Ellis RH (1997) Ex situ biodiversity conservation by seed storage: multiple-criteria keys to estimate seed storage behaviour. Seed Sci Technol 25:157–161Google Scholar
- Hong TD, Ellis RH (1998) Contrasting seed storage behavior among different species of Meliaceae. Seed Sci Technol 26:77–95Google Scholar
- Hong TD, Linington S, Ellis RH (2001) Compendium of information on seed storage behaviour, vol 1. Royal Botanic Gardens, Kew, p 400Google Scholar
- Roberts EH (1973) Predicting the storage life of seeds. Seed Sci Technol 1:499–514Google Scholar
- Tompsett PB (1994) Capture of genetic resources by collection and storage of seed: a physiological approach. In: Leakey RRB, Newton AC (eds) Tropical trees: the potential for domestication and the rebuilding of forest resources. HMSO, London, pp 61–71Google Scholar
- Tompsett PB, Kemp R (1996) Database of tropical tree seed research. Royal Botanic Gardens, KewGoogle Scholar
- Xia K, Daws MI, Stuppy W, Zhou ZK, Pritchard HW (2012) Rates of water loss and uptake in recalcitrant fruits of Quercus species are determined by pericarp anatomy. PLoS ONE 7(10):1–11Google Scholar