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New Forests

, Volume 48, Issue 4, pp 547–562 | Cite as

Diversity of arbuscular mycorrhizal fungi in Tectona grandis Linn.f. plantations and their effects on growth of micropropagated plantlets

  • Amornrat Chaiyasen
  • David D. Douds
  • Paiboolya Gavinlertvatana
  • Saisamorn Lumyong
Article
  • 272 Downloads

Abstract

Regeneration of stands of valuable tropical hardwood tree species for sustainable harvest requires production of seedlings with high probabilities of survival. One way to enhance the vigor of plants for outplanting is pre-colonization of roots by arbuscular mycorrhizal (AM) fungi. We pursued the strategy that the most promising AM fungus candidates for inoculation would be those associated with the tree of interest in the field. AM fungus communities were assessed in five plantations of Tectona grandis Linn.f. A total of 18 AM fungal morphotypes were found, representing four families: Glomeraceae (49.6%), Acaulosporaceae (24.9%), Claroideoglomeraceae (20.8%), and Gigasporaceae (4.8%). AM fungus spore density was negatively correlated with soil organic carbon. Some of these AM fungi, plus Rhizophagus irregularis, were established in pot culture and in vitro with transformed carrot roots, and subsequently used to inoculate micropropagated plantlets of T. grandis. Tectona grandis plantlets inoculated in vitro were successfully colonized by all AM fungi studied. Plants inoculated with Funneliformis mosseae were taller than uninoculated plants. Tectona grandis plantlets inoculated with the AM fungus Claroideoglomus etunicatum PBT03 were taller than uninoculated controls in ex vitro experiments. This study provides early insight for the targeted use of the AM symbiosis in production of important tree species in future greenhouse studies and reforestation.

Keywords

Arbuscular mycorrhizal fungi Micropropagated plant Rhizosphere soil Teak 

Notes

Acknowledgements

We would like to acknowledge the financial support of The Royal Golden Jubilee PhD Program (PHD/0150/2550), Thailand Research Fund (TRF), Research-Team Promotion Grant (RTA5880006), Chiang Mai University, and Center of Excellence on Biodiversity (BDC), Office of Higher Education Commission (BDC-PG2-159011).

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Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • Amornrat Chaiyasen
    • 1
    • 4
  • David D. Douds
    • 2
  • Paiboolya Gavinlertvatana
    • 3
  • Saisamorn Lumyong
    • 1
  1. 1.Department of Biology, Faculty of ScienceChiang Mai UniversityChiang MaiThailand
  2. 2.USDA, Agricultural Research Service, Eastern Regional Research CenterWyndmoorUSA
  3. 3.Thai Orchids Lab Co. Ltd.BangkokThailand
  4. 4.Soil Science Research Group, Agricultural Production Science Research and Development Division, Department of AgricultureMinistry of Agriculture and CooperativesBangkokThailand

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