Abstract
Dormancy in Juniperus polycarpos (K. Koch) seeds is a major hurdle for production of the desired quantity of seedlings in nurseries for restoration purpose. Thus, the aim of this study was to develop an optimal dormancy breaking treatment and subsequent stimulation of germination. The treatments applied were cold stratification at 1 °C for 4, 8, 12, and 16 weeks; warm stratification at 20 °C for 4, 8, 12, and 16 weeks followed by 12 weeks of cold stratification; exogenous application of 250, 500, and 1000 ppm gibberellic acid (GA3) and 6-Benzylamino purine (BAP); and a combination of 500 ppm hormones and cold stratification. The results show significant differences in germination between cold and warm-cold stratification, length of stratification and their interaction (p < 0.01). Warm stratification for 16 weeks followed by 12 weeks of cold stratification induced 72 % of the seeds to germination in 12 days compared with 16-week cold-stratification alone (42 %) and the control (8 %). Exogenous application of GA3 and BAP alone or in combination with cold stratification resulted in less than 50 % germination though significantly (p < 0.01) higher than the control. Apparently, the hormone treatments alone or in combination with cold stratification are not effective in completely breaking dormancy and stimulation of germination. The findings suggest that J. polycarpos seeds may possess intermediate simple morpho-physiological dormancy, which could be an adaptive mechanism for relatively warmer autumn temperature during seed maturation and the subsequent cold winter temperature under natural conditions.
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References
Adams RH (2014) Junipers of the World: the genus Juniperus, 4th edn. Trafford Publishing, Bloomington (Indiana)
Afroze F, O´Reilly C (2013) Breaking seed dormancy in European rowan seeds and its implications for regeneration. New For 44:547–557
Ahani H, Jalilvand H, Hosseini-Nasr SM, Soltani KH, Ghazi MR, Mohammadzadeh H (2013) Reproduction of Juniperus polycarpos in Khorasan Razavi. Iran For Sci Prac 15:231–237
Ahmadloo F, Kochaksaraei MT, Azadi P, Hamidi A, Beiramizadeh E (2015) Effects of pectinase, BAP and dry storage on dormancy breaking and emergence rate of Crataegus pseudoheterophylla Pojark. New For 46:373–386
Al-Refai A, El-kateb H, Stimm B, Mosandl R (2003) Quality and germination of seeds of J. excelsa M.-Bieb. In the Kalamoun, Syria. Forstliche Forschungsberichte Munchen. 192164-175. ISSN: 0174-1810
Alvarado V, Hiroyaki H, Bradford KJ (2000) Expression of endo-β-mannanase and SNF-related protein kinase genes in true potato seeds in relation to dormancy, gibberellin and abscisic acid. In: Viémont J-D, Crabbé J (eds) Dormancy in plants: from whole plant behavior to cellular control. CABI Publishing, Wallingford, pp 347–364
Baskin CC, Baskin JM (2001) Seeds: ecology, biogeography, and evolution of dormancy and germination. Academic Press, San Diego
Baskin JM, Baskin CC (2004) A classification system for seed dormancy. Seed Sci Res 14:1–16
Baskin CC, Zackrisson O, Baskin JM (2002) Role of warm stratification in promoting germination of seeds of Empetrum hermaphroditum (Empetraceae), a circumboreal species with a stony endocarp. Am J Bot 89:486–493
Bewley JD, Black M (1994) Seeds: physiology of development and germination. Plenum Press, New York
Chen SY, Chien CT, Hidayati SN, Walck JL (2014) Taiwanese montane Sambucus chinensis seeds require warm stratification, contrasting with other congeneric temperate members. Seed Sci Res 24:217–228
Daneshvar A, Tigabu M, Karimidoost A, Odén PC (2016). Flotation techniques for upgrading the viability of Juniperus polycarpos seed lots. J For Res (in press)
Downie B, Hilhorst HWM, Bewley JD (1997) Endo-β-mannanase activity during dormancy alleviation and germination of white spruce (Picea glauca) seeds. Physiol Plant 101:405–415
El-Juhany LI, Aref IM, Al-Ghamdi MA (2009) Effects of different pre-treatments on seed germination and early establishment of the seedling of Juniperus procera trees. World Appl Sci J 7:616–624
Finch-Savage W, Cadman C, Toorop P, Lynn J, Hilhorst H (2007) Seed dormancy release in Arabidopsis Cvi by dry after-ripening, low temperature, nitrate and light shows common quantitative patterns of gene expression directed by environmentally specific sensing. Plant J 51:60–78
Hidayati SN, Baskin JM, Baskin CC (2000) Dormancy-breaking and germination requirements for seeds of Diervilla lonicera (Caprifoliaceae), a species with underdeveloped linear embryos. Can J Bot 78:1199–1205
International Seed Testing Association (2010) International Seed Testing Association, International Rules for Seed Testing. Ch-8303 Bassersdorf, Switzerland
Korouri AAS, Khoshnevis M, Matinizadeh M (2012) Comprehensive studies of Juniperus species in Iran. Forest, Range and Watershed Management Organization (In Persian with English Abstract)
Kucera B, Cohn M, Leubner-Metzger G (2005) Plant hormone interactions during seed dormancy release and germination. Seed Sci Res 15:281–307
Lorenzo O, Rodríguez D, Nicolás G, Rodríguez PL, Nicolás C (2001) A new protein phosphatase 2C (FsPP2C1) induced by abscisic acid is specifically expressed in dormant beechnut seeds. Plant Physiol 125:1949–1956
Mamo N, Mihretu M, Fekadu M, Tigabu M, Teketay D (2006) Variation in seed and germination characteristics among Juniperus procera populations in Ethiopia. For Ecol Manag 225:320–327
Matilla A (2000) Ethylene in seed formation and germination. Seed Sci Res 10:111–126
Pawłowski TA (2010) Proteomic approach to analyze dormancy breaking of tree seeds. Plant Mol Biol 73:15–25
Santiago A, Ferrandis P, Herranz JM (2015) Non-deep simple morphophysiological dormancy in seeds of Viburnum lantana (Caprifoliaceae), a new dormancy level in the genus Viburnum. Seed Sci Res 25:46–56
Scianna JD (2001) Rocky Mountain juniper seed (collecting, processing and germination). Native Plants J 2(2):73–78
Shen TY, Odén PC (2002) Relationships between seed vigour and fumarase activity in Picea abies, Pinus contorta, Betula pendula and Fagus sylvatica. Seed Sci Techol 30:177–186
Sivakumar V, Anandalakshmi R, Warrier RR, Tigabu M, Odén PC, Vijayachandran SN, Geetha S, Singh BG (2006) Effects of presowing treatments, desiccation and storage conditions on germination of Strychnos nux-vomica seeds, a valuable medicinal plant. New For 32:121–131
Soltani A, Tigabu M, Odén PC (2005) Alleviation of physiological dormancy in oriental beechnuts with cold stratification at controlled and unrestricted hydration. Seed Sci Technol 33:283–292
Teketay D (1997) Chilling enhances seed germination of Rosa abyssinica. J Trop For 13:129–134
Tigabu M, Fjellström J, Odén PC, Teketay D (2007) Germination of Juniperus procera seeds in response to stratification and smoke treatments, and detection of insect-damaged seeds with VIS + NIR spectroscopy. New For 33:155–169
Tilki F (2007) Preliminary results on the effects of various pre-treatments on seed germination of Juniperus oxycedrus L. Seed Sci Technol 35:765–770
Tylkowski T (2009) Improving seed germination and seedling emergence in the Juniperus communis. Dendrobiology 61:47–53
Tylkowski T (2010) Dormancy breaking in Savin Juniper (Juniperus sabina L.) seeds. Acta Soc Bot Pol 79:27–29
Zar J (1996) Biostatistical analysis, 3rd edn. Prentice Hall Inc, Upper Saddle River
Acknowledgments
Authors thank Mostafa Khoshnevis and Bahram Naseri for valuable discussion. Employees of Iranian Agricultural and Natural Resources Research Centre (Golestan Province) are acknowledged for their help during seed collection. The first author was financial supported by the Iranian government scholarship program.
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Daneshvar, A., Tigabu, M., Karimidoost, A. et al. Stimulation of germination in dormant seeds of Juniperus polycarpos by stratification and hormone treatments. New Forests 47, 751–761 (2016). https://doi.org/10.1007/s11056-016-9542-8
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DOI: https://doi.org/10.1007/s11056-016-9542-8