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Physiological response to drought in seedlings of Pistacia lentiscus (mastic tree)

Abstract

Drought is often an important constraint on plant regeneration in the Mediterranean. Pistacia lentiscus is of particular interest in restoration actions in fire-prone areas. This study addressed the current knowledge gap on the physiological response of this species to drought during its initial development. We hypothesized that the seedlings would have a high resistance to drought. In addition, we expected that seedlings from two sources with contrasting climate conditions would perform distinctly under drought. Seeds were collected from two populations in north and south Portugal. Seedlings were subjected to a progressively lower osmotic potential during 25 days, through increasing PEG concentration in the hydroponic solution. The physiological response of the seedlings was assessed by measuring gas exchange and chlorophyll fluorescence after 15 and 25 days of drought exposure, when the osmotic potential was −2.47 and −5.17 MPa, respectively. Shoot growth, net CO2 assimilation rate, stomatal conductance and transpiration were significantly reduced under drought conditions, whilst intrinsic water-use efficiency (IWUE) remained unaffected. This response partially agreed with the known strategy of P. lentiscus to cope with drought, typically maintaining high IWUE, however the observed reduction in stomatal conductance was not as pronounced as expected. Drought induced a decrease in leaf pigments that could be linked to photo-protective mechanisms. Seed source did not have a significant effect on drought response. P. lentiscus showed a high resistance to drought, which clearly supported its use in restoration actions in the Mediterranean, but these findings require further testing under field conditions.

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Fig. 1

Abbreviations

A:

Net CO2 assimilation rate

E:

Transpiration rate

gs :

Stomatal conductance

Ci/Ca :

Ratio of intercellular to atmospheric CO2 concentration

IWUE:

Intrinsic water use efficiency

Fv/Fm :

Maximum photochemical efficiency of PSII

qP :

Photochemical quenching

ΦPSII :

Effective quantum efficiency of PSII

NPQ:

Non-photochemical quenching

WC:

Water content

CT:

Control

D:

Drought

OP:

Osmotic potential

MW:

Mann–Whitney

KW:

Kruskal–Wallis

References

  • Bacelar EA, Moutinho-Pereira JM, Gonçalves B, Ferreira H, Correia CM (2007) Changes in growth, gas exchange, xylem hydraulic properties and water use efficiency of three olive cultivars under contrasting water availability regimes. Environ Exp Bot 60:183–192

    Article  CAS  Google Scholar 

  • Bacelar E, Moutinho-Pereira JM, Gonçalves B, Brito C, Gomes-Laranjo J, Ferreira H, Correia CM (2012) Water use strategies of plants under drought conditions. In: Aroca Ricardo (ed) Plant responses to drought stress: from morphological to molecular features. Springer, Berlin, pp 145–170

    Chapter  Google Scholar 

  • Baeza MJ, Santana VM, Pausas JG, Vallejo VR (2011) Successional trends in standing dead biomass in Mediterranean basin species. J Veg Sci 22:467–474

    Article  Google Scholar 

  • Björkman O, Demmig-Adams B (1994) Regulation of photosynthetic light energy capture, conversion, and dissipation in leaves of higher plants. In: Schulze E-D, Caldwell MM (eds) Ecophysiology of Photosynthesis. Springer, Berlin, p 1747

  • Correia O, Barradas MD (2000) Ecophysiological differences between male and female plants of Pistacia lentiscus L. Plant Ecol 149:131–142

    Article  Google Scholar 

  • Cortina J, Green JJ, Baddeley JA, Watson CA (2008) Root morphology and water transport of Pistacia lentiscus seedlings under contrasting water supply: a test of the pipe stem theory. Environ Exp Bot 62:343–350

    Article  Google Scholar 

  • Filella I, Lusia J, Piñol J, Peñuelas J (1998) Pistacia lentiscus and Quercus ilex saplings in severe drought and high temperature conditions. Environ Exp Bot 39:213–220

    Article  Google Scholar 

  • Flexas J, Gulías J, Jonasson S, Medrano H, Mus M (2001) Seasonal patterns and control of gas exchange in local populations of the Mediterranean evergreen shrub Pistacia lentiscus L. Acta Oecol 22:33–43

    Article  Google Scholar 

  • Galmés J, Abadía A, Cifre J, Medrano H, Flexas J (2007) Photoprotection processes under water stress and recovery in Mediterranean plants with different growth forms and leaf habits. Physiol Plant 130:495–510

    Article  Google Scholar 

  • García-Fayos P (2001) Bases ecológicas para la recolección, almacenamiento y germinación de semillas de especies de uso forestal en la Comunidad Valenciana. Generalitat Valenciana, Valencia (Book ID: 6170)

  • García-Fayos P, Verdú M (1998) Soil seed bank factors controlling germination and establishment of a Mediterranean shrub: Pistacia lentiscus L. Acta Oecol 19:357–366

    Article  Google Scholar 

  • Gratani L, Varone L (2004) Adaptive photosynthetic strategies of the Mediterranean maquis species according to their origin. Photosynthetica 42:551–558

    Article  Google Scholar 

  • Gúlias J, Flexas J, Abadía A, Madrano H (2002) Photosynthetic responses to water deficit in six Mediterranean sclerophyll species: possible factors explaining the declining distribution of Rhamnus ludovici-salvatoris, an endemic Balearic species. Tree Physiol 22:687–697

  • IPCC (2013) Working group I: the physical basis of climate change. Cambridge University Press, Cambridge

    Google Scholar 

  • IPMA (Portuguese Institute of the Sea and Atmosphere). https://www.ipma.pt/pt/oclima/normais.clima/ (Last accessed Aug 2013)

  • Jordano P (1989) Pre-dispersal biology of Pistacia lentiscus (Anacardiaceae): cumulative effects on seed removal by birds. Oikos 55:375–386

    Article  Google Scholar 

  • Keeley JE, Bond WJ, Bradstock RA, Pausas JG, Rundel PW (2012) Fire in Mediterranean ecosystems. Cambridge University Press, New York, p 515

    Google Scholar 

  • Kyparissis A, Petropoulou Y, Manetas Y (1995) Summer survival of leaves in a soft-leaved shrub (Phlomis fruticosa L., Labiatae) under Mediterranean field conditions: avoidance of photoinhibitory damage through decreased chlorophyll contents. J Exp Bot 46:1825–1831

    Article  CAS  Google Scholar 

  • Liodakis S, Kakardakis T (2008) Measuring the relative particle foliar combustibility of WUI forest species located near Athens. J Therm Anal Calorim 93:627–635

    Article  CAS  Google Scholar 

  • Maestre FT, Cortina J, Bautista S, Bellot J, Vallejo R (2003) Small-scale environmental heterogeneity and spatiotemporal dynamics of seedling establishment in a semiarid degraded ecosystem. Ecosystems 6:630–643

    Article  Google Scholar 

  • Maxwell K, Johnson GN (2000) Chlorophyll fluorescence—a practical guide. J Exp Bot 51:659–668

    Article  CAS  PubMed  Google Scholar 

  • Michel BE, Kaufmann MR (1973) The osmotic potential of polyethylene glycol 6000. Plant Physiol 51:914–916

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Monteiro M, Santos C, Mann RM, Soares AM, Lopes T (2007) Evaluation of cadmium genotoxicity in Lactuca sativa L. using nuclear microsatellites. Environ Exp Bot 60:421–427

    Article  CAS  Google Scholar 

  • Moreno-Gutiérrez C, Dawson TE, Nicolás E, Querejeta JI (2012) Isotopes reveal contrasting water use strategies among coexisting plant species in a Mediterranean ecosystem. New Phytol 196:489–496

    Article  PubMed  Google Scholar 

  • Moutinho-Pereira JM, Correia CM, Gonçalves B, Bacelar EL, Torres-Pereira JM (2004) Leaf gas-exchange and water relations of grapevines grown in three different conditions. Photosynthetica 42:81–86

    Article  Google Scholar 

  • Murata N, Takahashi S, Nishiyama Y, Allakhverdiev SI (2007) Photoinhibition of photosystem II under environmental stress. Biochim et Biophys Acta (BBA)-Bioenerg, 1767(6):414-421

  • Osmond CB, Anderson JM, Ball MC, & Egerton JJG (1999) Compromising efficiency: the molecular ecology of light-resource utilization in plants. Physiol plant ecol. Blackwell Science Ltd, Oxford, 1–25.

  • Paula S, Pausas JG (2008) Burning seeds: germinative response to heat treatments in relation to resprouting ability. J Ecol 96:543–552

    Article  Google Scholar 

  • Pausas J, Llovet J, Rodrigo A, Vallejo R (2008) Are wildfires a disaster in the Mediterranean basin? A review. Int J Wildland Fire 17:713–723

    Article  Google Scholar 

  • Santos C, Fragoeiro S, Phillips A (2005) Physiological response of grapevine cultivars and a rootstock to infection with Phaeoacremonium and Phaeomoniella isolates: an in vitro approach using plants and calluses. Sci Hortic 103:187–198

    Article  CAS  Google Scholar 

  • Sims DA, Gamon JÁ (2002) Relationships between leaf pigment content and spectral reflectance across a wide range of species, leaf structures and developmental stages. Remote Sens Environ 81:337–354

    Article  Google Scholar 

  • Tsakaldimi M, Ganatsas P (2001) Treatments improving seed germination of two Mediterranean sclerophyll species: Ceratonia siliqua and Pistacia lentiscus. In: Proceedings of the 3rd Balcan scientific conference, vol II, pp 119–127. ISBN: 954-90896-3-0

  • Valladares F, Sánchez-Gómez D (2006) Ecophysiological traits associated with drought in Mediterranean tree seedlings: individual responses versus interspecific trends in eleven species. Plant Biol 8:688–697

    Article  CAS  PubMed  Google Scholar 

  • Vallejo VR, Smanis A, Chirino E, Fuentes D, Valdecantos A, Vilagrosa A (2012) Perspectives in dryland restoration: approaches for climate change adaptation. New For 43:561–579

    Article  Google Scholar 

  • Vasques A, Chirino E, Vilagrosa A, Vallejo VR, Keizer JJ (2013) The role of seed provenance in the early development of Arbutus unedo seedlings under contrasting watering conditions. Environ Exp Bot 96:11–19

    Article  Google Scholar 

  • Vasques A, Vallejo VR, Santos C, Keizer JJ (2014) The role of cold storage and seed source in the germination of three Mediterranean shrub species with contrasting dormancy types. Ann For Sci. doi:10.1007/s13595-014-0395-z

    Google Scholar 

  • Verdú M, García-Fayos P (1998a) Female biased sex ratios in Pistacia lentiscus L. (Anacardiaceae). Plant Ecol 135:95–101

    Article  Google Scholar 

  • Verdú M, García-Fayos P (1998b) Ecological causes, function, and evolution of abortion and parthenocarpy in Pistacia lentiscus (Anacardiaceae). Can J Bot 76:134–141

    Google Scholar 

  • Verdú M, García-Fayos P (2000) The effect of deceptive fruits on predispersal seed predation by birds in Pistacia lentiscus. Plant Ecol 156(2):245–248

  • Vilagrosa A, Morales F, Abadía A, Bellot J, Cochard H, Gil-Pelegrin E (2010) Are symplast tolerance to intense drought conditions and xylem vulnerability to cavitation coordinated? An integrated analysis of photosynthetic, hydraulic and leaf level processes in two Mediterranean drought-resistant species. Environ Exp Bot 69:233–242

    Article  Google Scholar 

  • Vilagrosa A, Hernández EI, Luis VC, Cochard H, Pausas JG (2014) Physiological differences explain the co-existence of different regeneration strategies in Mediterranean ecosystems. New Phytol 201:1277–1288

    Article  PubMed  Google Scholar 

  • Zohary M (1952) A monographical study of the genus Pistacia. Palest J Bot Jerusalem Ser 5:187–228

  • Zunzunegui M, Barradas MCD, Ain-Lhout F, Alvarez-Cansino L, Esquivias MP, Novo FG (2011) Seasonal physiological plasticity and recovery capacity after summer stress in Mediterranean scrub communities. Plant Ecol 212:127–142

    Article  Google Scholar 

Download references

Acknowledgments

The authors would like to thank Diana Vieira, Xufeng Shen, Carlota Vaz and Mariana Pedro for their kind help provided in seed harvesting and to Jingxian Tao, Armando Costa, Cristina Monteiro, Paula Maia and Nelson Abrantes for their laboratory support. We are also very grateful to Banc de Llavors Forestalls de Valencia (Spain) for the indications on the seed handling protocols and to the reviewers and editors for their valuable comments. This study was funded by the Portuguese Foundation for Science and Technology (FCT) and QREN through the PhD grant of the first author (SFRH/BD/47522/2008) and the post-doctoral fellowship of M.C. Dias (SFRH/BPD/41700/2007). The post-doctoral fellowship of G. Pinto (BPD/UI88/2154/2014) was funded by Centro de Estudos do Ambiente e Mar-CESAM- INCENTIVO. The review process of this manuscript received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under Grant Agreement No. 283068 (CASCADE project).

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Vasques, A.R., Pinto, G., Dias, M.C. et al. Physiological response to drought in seedlings of Pistacia lentiscus (mastic tree). New Forests 47, 119–130 (2016). https://doi.org/10.1007/s11056-015-9497-1

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  • DOI: https://doi.org/10.1007/s11056-015-9497-1

Keywords

  • Osmotic stress
  • Polyethylene glycol
  • Ecological restoration
  • Resilience