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Effects of water stress induced by sodium chloride and mannitol on proline accumulation, photosynthetic abilities and growth characters of eucalyptus (Eucalyptus camaldulensis Dehnh.)

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Abstract

The aim of this study was to investigate the biochemical, physiological and morphological responses of eucalypts to iso-osmotic salt and water deficit stress. Chlorophyll a, chlorophyll b, total chlorophyll (TC), total carotenoids, maximum quantum yield of PSII (F v /F m ), photon yield of PSII and non-photochemical quenching, in severely osmotic-stressed plantlets (−1.20 MPa) decreased significantly when compared to control plantlets, leading to low net photosynthetic rate (P n ) and growth reduction. In addition, the photosynthesis and growth parameters of salt stressed plantlets declined to a greater degree than those cultivated in water-deficit conditions. On the other hand, the proline content of the osmotic-stressed leaves increased significantly, especially under iso-osmotic salt stress conditions. The TC content of iso-osmotic stressed leaves was significantly degraded, with low F v /F m , leading to P n reduction and growth inhibition. Those parameters in salt stressed plantlets were significantly lower and to a greater degree than in plantlets subjected to water-deficit. The basic knowledge of biochemical, physiological and morphological changes in responses to iso-osmotic salt and water-deficit stresses will be further applied as effective indices for salt and water-deficit tolerant screening in large population of eucalyptus breeding program.

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Acknowledgments

The authors are grateful to Jonathan Shore for grammatical proofing. This experiment was funded by the Siam Cement Group (SCG) and partially supported by the National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA).

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  1. National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Paholyothin Road, Klong 1, Klong Luang, Pathumthani, 12120, Thailand

    Suriyan Cha-um & Chalermpol Kirdmanee

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  1. Suriyan Cha-um
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Correspondence to Suriyan Cha-um.

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Cha-um, S., Kirdmanee, C. Effects of water stress induced by sodium chloride and mannitol on proline accumulation, photosynthetic abilities and growth characters of eucalyptus (Eucalyptus camaldulensis Dehnh.). New Forests 40, 349–360 (2010). https://doi.org/10.1007/s11056-010-9204-1

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