Abstract
The effect of seed water content (WC) (2–3, 5–6 and 22–25%, on a fresh weight basis), storage temperature (+4, −20, −80 and −196°C) and storage duration (6, 12, 24, 48 and 60 months) on the germination of white spruce (Picea glauca (Moench) Voss), black spruce (Picea mariana (Mill.) B.S.P.) and lodgepole pine (Pinus contorta Dougl. ex Loud. var. latifolia Engelm.) seed was investigated. Germination of white spruce control (untreated) seeds and seeds adjusted to 2–3% and 5–6% WC declined after 48 months of storage at −80 and −196°C, with a further decline at 60 months at −20, −80, −196°C. Germination remained high when control white spruce seeds and seeds with 2–3, 5–6% WC were stored at +4°C, over all storage durations. Generally, black spruce and lodgepole pine exhibited high germination at all storage temperatures at 2–3% and 5–6% WC as well as the control (untreated) seed, for up to 60 months in storage. Germination declined for all three species when seed was conditioned to 22–25% WC. This loss in germination was partially recovered in white spruce seed stored at +4, −20 and −80°C after storage durations of 24, 12 and 48 months, respectively, and in black spruce seeds stored at −20 and −196°C after storage durations of 24 months. Mean germination time (MGT) was relatively constant for all species, under all conditions, except for seed conditioned to 22–25% WC, where MGT increased for white spruce seed stored 48 months at −80 and −196°C, and for black spruce seed stored 24 months at +4 and −80°C and 60 months at −196°C. These results show that the optimal storage temperatures are 4°C for white spruce, and 4, −20, −80, and −196°C for black spruce and lodgepole pine, and 2–6% water content is optimal for all 3 species at these temperatures.
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References
Allen GS (1957) Storage behaviour of conifer seeds in sealed containers held at 0°F, 32°F, and room temperature. J For 55:278–285
Allen GS, Bientjes W (1954) Studies on coniferous tree seed at the University of British Columbia. For Chron 30:183–196
AOSA (Association of Official Seed Analysis) (1985) Rules for testing seeds. J Seed Technol 6:1–118
Bewley JD, Black M (1994) Seeds. Physiology of development and germination, 2nd edn. Plenum Press, New York, pp 388–408
Bonner FT (1990) Storage of seeds: potential and limitations for germplasm conservation. For Ecol Manage 35:35–43
Bonner FT (1994) Predicting seed longevity for four forest tree species with orthodox seeds. Seed Sci Technol 22:361–370
Buitink J, Leprince O, Hemmings MA, Hoekstra FA (2000) Molecular mobility in the cytoplasm: an approach to describe and predict lifespan of dry germplasm. Proc Natl Acad Sci USA 5:2385–2390
Chen SY, Chien CT (2002) Germination and storage behaviour of Prunus phaeosticta and Prunus spinulosa seeds stored. Taiwan J For Sci 1:59–66
Dickie JB, Ellis RH, Kraak HL, Ryder K, Tompsett PB (1990) Temperature and seed storage longevity. Ann Bot 65:197–204
Edwards DGW (1977) Tree seed research, Pacific Forest Research Centre. In: Proceedings, Sixteenth Meeting Canadian Tree Improvement Association, Part 1. Winnipeg, MB, 27–30 June 1977, pp 209–216
Edwards DGW (1986) Special prechilling techniques for tree seeds. J Seed Technol 10:151–171
Edwards DGW, El-Kassaby YA (1988) Effect of flowering phenology, date of cone collection, cone-storage treatment and seed pretreatment on yield and germination of seeds from a Douglas-fir seed orchard. For Ecol Manage 25:17–29
Ellis RH, Roberts EH (1980a) Improved equations for the prediction of seed longevity. Ann Bot 45:13–30
Ellis RH, Roberts EH (1980b) The influence of temperature and moisture on seed viability period in barley (Hordeum distichum L.). Ann Bot 45:31–37
Fraser JW (1971) Cardinal temperatures for germination of six provenances of white spruce seeds. Department of Fisheries and Forestry, Canadian Forestry Service Publication No. 1290
Haais FW, Thrupp AC (1931) Temperature relations of lodgepole pine seed germination. Ecology 12:728–744
Harrington JF (1972) Seed storage and longevity. In: Kozlowski TT (ed) Seed biology, vol 3. Academic Press, New York, pp 145–240
Hilli A, Tillman-Sutela E, Kauppi A (2003) Germination of pretreated Scots pine seeds after long-term storage. Can J For Res 33:47–53
Hor YL, Kim YJ, Ugap A, Chabrillange N, Sinniah UR, Engelmann F, Dussert S (2005) Optimal hydration status for cryopreservation of intermediate oily seeds: citrus as a case study. Ann Bot 95:1153–1161
Ibrahim AE, Roberts EH (1983) Viability of lettuce seeds. I. Survival in hermetic storage. J Exp Bot 34:620–630
International Seed Testing Association (ISTA) (1985) International rules for seed testing. Seed Sci Technol 13:300–513
Ishikawa M, Sakai A (1978) Freezing avoidance by deep supercooling in hydrated lettuce seeds. Nature 269:325–327
Jeffers RM (1992) Seed quality in five jack pine in north central Wisconsin. North Central Forest Experimental Station Research Note NC-328:1–5
Jensen LA, Noll E (1959) Experience of germination testing of Pacific Northwest Douglas fir seed. Association of Official Seed Analysts Proceedings 49:107–113
Jones L (1962) Recommendations for successful storage of tree seeds. Tree Planters Notes 55:9–20
Jones SK, Gosling PG (1994) Target moisture content pre-chill overcomes the dormancy of temperature conifer seeds. New Forest 8:309–321
Krugman SL, Jenkinson JL (1974) Pinus. In: Seeds of woody plants in the United States. Forest Service, USDA, Washington, DC., pp 598–638
Lehner A, Corbineau F, Bailly C (2006) Changes in lipid status and glass properties in cotyledons of developing sunflower seeds. Plant Cell Physiol 47:818–828
Li XJ, Burton PJ, Leadman CL (1994) Interactive effects of light and stratification on the germination of some British Columbia conifers. Can J Bot 72:1635–1646
Morgenstern EK (1969) Genetic variation in seedlings of Picea mariana (Mill.) B.S.P. I. Correlation with ecological factors. Silvae Genetica 18:151–161
Muller C, Falleri E, Laroppe E, Bonnet-Masimbert M (1999) Drying and storage of prechilled Douglas fir, Pseudotsuga menziesii, seeds. Can J For Res 29:172–177
Probert RJ, Hay FR (2000) Keeping seed alive. In: Black M, Bewley JD (eds) Seed technology and its biological basis. Sheffield Academic Press, England, pp 375–411
Pritchard HW, Dickie JB (2003) Predicting seed longevity: the use and abuse of seed viability equations. In: Seed conservation: turning science into practice. The Royal Botanical Gardens Publishers, Kew, UK, pp 653–721
Rawat BS, Sharma CM, Ghildiyal SK (2006) Improvement of seed germination in tree important conifer species by gibberellic acid. Iyonia 11:23–30
Roberts EH (1973) Predicting the storage life of seeds. Seed Sci Technol 1:499–514
Roberts EH, Ellis RH (1989) Water and seed survival. Ann Bot 63:39–52
Sarvas R (1967) Climatological control of flowering in trees. In: Proceedings XIV IUFRO conference, Section 22_AQ 22/24. IUFRO, Vienna, pp 15–30
Sarvas R (1970) Temperature sum as a restricting factor in the development of forests in the Subarctic. In: The ecology for Subarctic regions. Proceedings UNESCO Symposium 1970, Helsinki, Finland. UNESCO, Paris, pp 79–82
SAS Institute (1995) SAS/STAT guide, Version 6 edn. SAS Institute, Cary, NC
Skeates DA, Irving DE, Haavisto VF (1989) Deterioration of black spruce seed during in situ storage and processing. Tree Planters Notes 40:5–8
Simola LK (1974) Ultrastucture changes in the seeds of Pinus sylversteris L. during senescence. Stud For Suec 119:1–22
Stanwood PC (1985) Cryopreservation of seed germplasm for genetic conservation. In: Kartha KK (ed) Cryopreservation of plant cells and organs. CRC Press Inc., Boca Raton, pp 199–226
Stanwood PC (1987) Survival of sesame seeds at the temperature (−196°C) of liquid nitrogen. Crop Sci 27:327–331
Styles ED, Burgess JM, Mason C, Huber BM (1982) Storage of seed in liquid nitrogen. Cryobiology 19:195–199
Thomson AJ, El-Kassaby Y (1993) Interpretation of seed-germination parameters. New Forest 7:123–132
Tompsett PB (1986) The effect of temperature and moisture content on the longevity of seeds of Ulmus carpinifolia and Terminalia brasii. Ann Bot 57:875–883
US Forest Service (1974) Seeds of woody plants in the United States. pp 872 in Agricultural Handbook No. 450. USDA Forest Service
Vertucci CW, Roos CW (1991) Theoretical basis of protocols for seed storage. Plant Physiol 94:1019–1023
Vertucci CW, Roos EE, Crane J (1994) Theoretical basis of protocols for seed storage III. Optimum moisture contents for pea seeds stored at different temperatures. Ann Bot 74:531–540
Villiers TA, Edgcumbe DJ (1975) On the cause of seed deterioration in dry storage. Seed Sci Technol 3:761–774
Walters C (2004) Temperature dependency of molecular mobility in preserved seeds. Biophys J 86:1253–1258
Walters C, Wheeler L, Stanwood P (2004) Longevity of cryogenically-stored seeds. Cryobiology 48:229–244
Wang BSP (1973) Laboratory germination criteria for red pine (Pinus resinosa Ait.) seed. Proceedings Association Official Seed Analysis 63:94–101
Wang BSP (1982) Long term storage of Abies, Betula, Larix, Picea, Pinus and Populus seeds. In: Proceedings symposium on conservation of forest gene resources. 13th Management committee on forest tree breeding. Canadian Forestry Service, Canada, pp 23–27
Wang BSP, Ackerman F (1983) A new germination box for tree seed testing. Canadian Forestry Service Information Report. PI-X-27
Wang BSP, Berjak P (2000) Beneficial effects of moist chilling on the seeds of black spruce (Picea mariana [Mill.] B.S.P.). Ann Bot 86:29–36
Wang BSP, Scheer GC, Coleman SJ (1993a) Effect of moisture content and storage temperature on germination of white spruce seeds. In: Tree seed problems, with special reference to Africa. Proceedings of the IUFRO symposium of the project group P.2.04.00 Ouagadougou, Burkina Faso, November 23–28
Wang BSP, Charest PJ, Downie B (1993b) Ex situ storage of seeds, pollen and in vitro cultures of perennial woody plant species. In: FAO Forestry Paper, 113. Food and Agricultural Organization of the United Nations, Rome Italy, pp 1–64
Winston PW, Bates DH (1960) Saturation salt solutions for the control of humidity in biological research. Ecology 41:232–237
Zen Y (2005) Functional analysis of a conifer ABI3 gene, an ABA signalling component regulating seed dormancy and germination. Ph.D. dissertation, Department of Biology, Simon Fraser University, 160 p
Zheng G, Jing X, Tao K (1998) Ultradry seed storage cuts cost of gene bank. Nature 393:223–224
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We would like to thank Dale Simpson and Dr. Sharon Pond for their valuable comments on the manuscript.
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Beardmore, T., Wang, B.S.P., Penner, M. et al. Effects of seed water content and storage temperature on the germination parameters of white spruce, black spruce and lodgepole pine seed. New Forests 36, 171–185 (2008). https://doi.org/10.1007/s11056-008-9091-x
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DOI: https://doi.org/10.1007/s11056-008-9091-x