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Abscisic acid decreases expression of COX-2 and TNF-α proteins in SH-SY5Y neuroblastoma cells as an in vitro model of Parkinson's disease

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Abstract

Parkinson's disease (PD) is one of the most common motor disorders in humans, associated with damage to the dopaminergic cells in the brain. Parkinson's disease is caused by chronic inflammation, mitochondrial dysfunction, oxidative stress and loss of dopaminergic neuronal cells in the dense part of the basal ganglia. In the present study, the neuroprotective effect of Abscisic acid (ABA) on 6-hydroxy-dopamine (6-OHDA)-induced cellular damage in dopaminergic SH -SY5Y cells of neuroblastoma as an in vitro model of Parkinson's disease was investigated. Since abscisic acid has anti-inflammatory effects, this study investigated the effect of ABA on neuronal inflammation by 6-hydroxydopamine in vitro. To induce cytotoxicity, the above cells were treated with 150 μM 6-hydroxydopamine, then the effective dose of ABA (100 μM) was used in the treatment groups. Then the biochemical parameters of neuronal inflammation (COX -2 and TNF-α) were measured. The results of this study showed that 6-hydroxy-dopamine increased the cellular damage and expression of COX -2 and TNF-α, and treatment with an effective dose of ABA (100 μM) reduced the aforementioned cellular and molecular abnormalities. Therefore, it appears that ABA has a neuroprotective effect against 6-hydroxydopamine-induced neuronal inflammation in vitro.

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Acknowledgments

The authors express their gratitude to the staff of Neuroscience Research Center, Kerman University of Medical Sciences.

Funding

This project was supported by a grant from the Neuroscience Research Center of Kerman University of Medical Sciences, Kerman, Iran.

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Authors and Affiliations

  1. Laboratory of Molecular Neuroscience, Neuroscience Research Centre, Institute of Neuropharmacology, Kerman University of Medical Sciences, Ebn Sina Avenue, Kerman, Iran

    Kiana Rafiepour, Pouyan Bahadori & Marzieh Maneshian

  2. Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran

    Ali Salehzadeh

  3. Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran

    Saeed Esmaeili-Mahani

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  1. Kiana Rafiepour
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  2. Ali Salehzadeh
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  4. Saeed Esmaeili-Mahani
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All the authors met the standard writing criteria based on the recommendations of International Committee of Medical Journal Publishers.

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Correspondence to Kiana Rafiepour.

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Rafiepour, K., Salehzadeh, A., Bahadori, P. et al. Abscisic acid decreases expression of COX-2 and TNF-α proteins in SH-SY5Y neuroblastoma cells as an in vitro model of Parkinson's disease. Neurosci Behav Physi 53, 688–694 (2023). https://doi.org/10.1007/s11055-023-01362-3

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  • DOI: https://doi.org/10.1007/s11055-023-01362-3

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