A miniature fluorescence microscope (miniscope) is a promising tool for visualizing in vivo neuronal activity in behaving animals. The data obtained by a miniscope contain massive amounts of information about neuronal activity. However, the extraction and analysis of these data are complex tasks. There are various difficulties in the processing of data obtained by a miniscope, both in the extraction of neural activity data and in the subsequent analysis. A software page, constrained nonnegative matrix factorization for microendoscopic data (CNMF-E), was developed previously to assist in the processing of miniscope data. In this paper, we present a novel software package, NeuroInfoViewer (NIV), for high-level analysis and visualization of miniscope data following initial processing. We present an example of the analysis data flow, from raw miniscope imaging data to CNMF-E and to NIV. We suggest that NIV may serve as a useful tool for high-level analysis of miniscope data and we have deposited NIV in the public domain to facilitate its use by the neuroscience community.
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Erofeev, A.I., Barinov, D.S., Gerasimov, E.I. et al. NeuroInfoViewer: A Software Package for Analysis of Miniscope Data. Neurosci Behav Physi 51, 1199–1205 (2021). https://doi.org/10.1007/s11055-021-01179-y
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DOI: https://doi.org/10.1007/s11055-021-01179-y