Characteristics of the Behavioral Phenotype of BALB/C Mice

The existence of specific behavioral characteristics which can be regarded as autistic spectrum disorder (ASD) in BALB/c mice was detected and confirmed, these consisting of a low level of interaction with socially relevant stimuli and even avoidance of this interaction, along with impairment to the discrimination and particularly the recognition of odors, elevated anxiety, and stereotypical behavior. BALB/c mice provide an experimental model of idiopathic ASD for studies of the pathogenesis of the diseases and its phenotypic manifestations, as well as for evaluating potential substances for the correction of ASD.

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  1. 1.

    Y. Avraham, E. M. Berry, M. Donskoy, et al., “Beta-carotene as a novel therapy for the treatment of ‘Autistic like behavior’ in animal models of autism,” Behav. Brain Res., 364, 469–479 (2019).

    CAS  Article  Google Scholar 

  2. 2.

    T. M. Kazdoba, P. T. Leach, Yang Mu, et al., “Translational mouse models of autism: advancing toward pharmacological therapeutics,” Curr. Top. Behav. Neurosci., 28, 1–52 (2016).

    CAS  PubMed  PubMed Central  Google Scholar 

  3. 3.

    E. S. Brodkin, “BALB/c mice: Low sociability and other phenotypes that may be relevant to autism,” Behav. Brain Res., 176, 53–65 (2007).

    CAS  Article  Google Scholar 

  4. 4.

    S. S. Moy, J. J. Nadler, A. Perez, et al., “Sociability and preference for social novelty in five inbred strains: an approach to assess autistic-like behavior in mice,” Genes Brain Behav., 3, No. 5, 287–302 (2004).

    CAS  Article  Google Scholar 

  5. 5.

    S. S. Moy, J. J. Nadler, N. B. Young, et al., “Mouse behavioral tasks relevant to autism: phenotypes of 10 inbred strains,” Behav. Brain Res., 176, No. 1, 4–20 (2007).

    Article  Google Scholar 

  6. 6.

    J. L. Silverman, M. Yang, K. Lord, and J. N. Crawley, “Behavioral phenotyping assay for mouse models of autism,” Nat. Rev. Neurosci., 11, No. 7, 490–502 (2010).

    CAS  Article  Google Scholar 

  7. 7.

    S. S. Moy, J. J. Nadler, M. D. Poe, et al., “Development of a mouse test for repetitive, restricted behaviors: relevance to autism,” Behav. Brain Res., 188, No. 1, 178–194 (2008).

    CAS  Article  Google Scholar 

  8. 8.

    J. N. Crawley, “Mouse behavioral assays relevant to the symptoms of autism,” Brain Pathol., 17, 448–459 (2007).

    Article  Google Scholar 

  9. 9.

    J. N. Crawley, T. Chen, A. Puri, et al., “Social approach behaviors in oxytocin knockout mice: comparison of two independent lines tested in different laboratory environments,” Neuropeptides, 41, No. 3, 145–163 (2007).

    CAS  Article  Google Scholar 

  10. 10.

    K. K. Chadman, S. Gong, M. L. S. Scattoni, et al., “Minimal aberrant behavioral phenotypes of neuroligin-3 R451C knockin mice,” Autism Res., 1, 147–158 (2008).

    Article  Google Scholar 

  11. 11.

    F. I. Roullet and J. N. Crawley, “Mouse models of autism: testing hypotheses about molecular mechanisms,” Curr. Top. Behav. Neurosci., 7, 187–212 (2011).

    Article  Google Scholar 

  12. 12.

    C. R. Shah, C. G. Forsberg, J. Kang, and J. Veenstra-Vanderweele, “Letting a typical mouse judge whether mouse social interactions are atypical,” Autism Res., 6, No. 3, 212–220 (2013).

    Article  Google Scholar 

  13. 13.

    J. A. Burket, C. M. Young, T. L. Green, et al., “Characterization of gait and olfactory behaviors in the BALB/c mouse model of autism spectrum disorders,” Brain Res. Bull., 122, 29–34 (2016).

    Article  Google Scholar 

  14. 14.

    F. Muratori, A. Tonacci, L. Billeci, et al., “Olfactory processing in male children with autism: atypical odor threshold and identification,” J. Autism Dev. Disord., 47, No. 10, 3243–3251 (2017).

    Article  Google Scholar 

  15. 15.

    K. Radyushkin, K. Hammerschmidt, S. Boretius, et al., “Neuroligin-3-deficient mice: model of a monogenic heritable form of autism with an olfactory deficit,” Genes Brain Behav., 8, 416–425 (2009).

    CAS  Article  Google Scholar 

  16. 16.

    E. H. Gregory and D. W. Pfaff, “Development of olfactory-guided behavior in infant rats,” Physiol. Behav., 6, No. 5, 573–576 (1971).

    CAS  Article  Google Scholar 

  17. 17.

    M. Yang and J. N. Crawley, “Simple behavioral assessment of mouse olfaction,” Curr. Protoc. Neurosci., Chapter 8: Unit-8.24 (2009).

  18. 18.

    B. C. Ryan, N. B. Young, J. N. Crawley, et al., “Social deficits, stereotypy and early emergence of repetitive behavior in the C58/J inbred mouse strain,” Behav. Brain Res., 208, 178–188 (2010).

    Article  Google Scholar 

  19. 19.

    E. V. Koplik, P. M. Salieva, and A. V. Gorbunova, “The open field test as a prognostic criterion for resistance to emotional stress in Wistar rats,” Zh. Vyssh. Nerv. Deyat., 45, No. 4, 775–781 (1995).

    CAS  Google Scholar 

  20. 20.

    H. Arakawa, “Involvement of serotonin and oxytocin in neural mechanism regulating amicable social signal in male mice: Implication for impaired recognition of amicable cues in BALB/c strain,” Behav. Neurosci., 131, No. 2, 176–191 (2017).

    CAS  Article  Google Scholar 

  21. 21.

    J. Ellegood and J. N. Crawley, “Behavioral and neuroanatomical phenotypes in mouse models of autism,” Neurotherapeutics, 12, 521–533 (2015).

    CAS  Article  Google Scholar 

  22. 22.

    M. A. Yarkova and S. B. Seredenin, “Temporal characteristics of stress-induced decrease in benzodiazepine reception in C57BL/6 and BALB/c mice,” Bull. Exp. Biol. Med., 157, No. 6, 733–735 (2014).

    Article  Google Scholar 

  23. 23.

    D. S. Melkumyan, T. S. Seredenina, M. A. Yarkova, et al., “Analysis of BDNF in brain structures of inbred mice with different phenotypes of mental and stress reaction,” Bull. Exp. Biol. Med., 140, No. 11, 549–551 (2005).

    Google Scholar 

  24. 24.

    M. L. Scattoni, A. Martire, G. Cartocci, et al., “Reduced social interaction, behavioural flexibility and BDNF signalling in the BTBR T + tf/J strain, a mouse model of autism,” Behav. Brain Res., 251, 35–40 (2013).

    CAS  Article  Google Scholar 

  25. 25.

    E. L. Grigorenko, Autism Spectrum Disorders. An Introductory Course: Textbook, Praktika, Moscow (2018).

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Correspondence to I. G. Kapitsa.

Additional information

Translated from Rossiiskii Fiziologicheskii Zhurnal imeni I. M. Sechenova, Vol. 106, No. 3, pp. 373–383, March, 2020.

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Kapitsa, I.G., Ivanova, E.A., Voronina, T.A. et al. Characteristics of the Behavioral Phenotype of BALB/C Mice. Neurosci Behav Physi 51, 93–99 (2021).

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  • BALB/c mice
  • autistic spectrum disorders
  • social deficit
  • stereotypy
  • impaired olfaction
  • anxiety