This review considers the current methods required for functional mapping of neural networks in 3D space with cellular resolution. Cyclic immunohistochemistry approaches and immunohistochemical staining methods for thick nervous tissue preparations are described in detail, along with approaches to clearing large brain formations to allow signals to be visualized at great depth. The main advantages and disadvantages of their use are considered. Perspectives for the development of molecular mapping for studies of the neural substrates of cognitive functions in health and disease are discussed.
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Translated from Zhurnal Vysshei Nervnoi Deyatel’nosti imeni I. P. Pavlova, Vol. 68, No. 6, pp. 747–758, November–December, 2018.
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Efimova, O.I., Balaban, P.M. & Khaitovich, F.E. Novel Approaches to the Molecular Mapping of the Brain: 3D Cyclic Immunohistochemistry and Optical Clearing. Neurosci Behav Physi 50, 73–80 (2020). https://doi.org/10.1007/s11055-019-00871-4
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DOI: https://doi.org/10.1007/s11055-019-00871-4