Use of Anchoring Motifs to Support the Central and Peripheral Locations of Opsins in Optogenetics
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One version of the optogenetic prosthetization of the degenerative retina is an approach based on creation of an ON/OFF receptive field for ganglion neurons by targeting the expression of an excitatory light-activated protein in the central part of the ganglion cell and an inhibitory protein at the periphery. Within the framework of this approach, we investigated the possibility of using various anchoring motifs to support the somatic or dendritic location of opsins. The motif of the Kv2.1 potassium channel does not always provide an exclusively central location for the light-activated protein, which may be linked with significant overexpression of the construct when powerful universal promoters are used. In addition, we have demonstrated the possibility of using a postsynaptic density protein, Homer1, as the anchoring motif for peripheral targeting of rhodopsins.
Keywordsoptogenetics optogenetic prosthetization retina anchoring motif neuron channelrhodopsin-2
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