Neurodegenerative diseases result from a complex interaction between unfavorable environmental factors and the individual characteristics of the genome which predispose to disease development. Drosophila provides a suitable system for studies of the relationship between genomic organization and chromosomal architecture producing cognitive impairments. We present here the results of complex investigations of the effects of attenuation of the geomagnetic field (by screening) on genomic transcriptional activity, learning ability, and the formation of intermediate-term memory in Drosophila melanogaster. In this stress, a relationship was seen between modifications to transcriptional activity and the structure of the LIMK1 gene – a key enzyme in the actin remodeling cascade. Impairments to intermediate-term memory were seen on exposure to a weak static magnetic field in Canton-S wild-type flies. Conversely, this same stress restored learning ability and memory formation in agnts3 mutants.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bamburg, J. R. and Zheng, J. Q., “ADF/cofilin-mediated actin dynamics regulate AMPA receptor trafficking during synaptic plasticity,” Nat. Neurosci., 13, No. 10, 1208–1215 (2010).
Bamburg, J. R., Bernstein, B. W., Davis, R. C., et al., “ADF/Cofilin-actin rods in neurodegenerative diseases,” Curr. Alzheimer Res., 7, No. 3, 241–250 (2010).
Baranov, V. S., “Genomics on the pathway to predictive medicine,” Acta Naturae, 1, No. 3, 77–88 (2009).
Cook, C. M., Saucier, D. M., Thomas, A. W., and Prato, F. S., “Exposure to ELF magnetic and ELF-modulated radiofrequency fields: the time course of physiological and cognitive effects observed in recent studies (2001–2005),” Bioelectromagnetics, 27, No. 8, 613–27 (2006).
Darieva, Z., Bulmer, R., Pic-Taylor, A., et al., “Polo kinase controls cellcycle-dependent transcription by targeting a coactivator protein,” Nature, 23, 494–498 (2006).
Davis, R. C., Marsden, I. T., Maloney, M. T., et al., “Amyloid beta dimers/trimers potently induce cofilin-actin rods that are inhibited by maintainin cofilin phosphorylation,” Mol. Neurodegener., 6, 10 (2011).
Eberharter, A. and Becker, P. B., “Histone acetylation: a switch between repressive and permissive chromatin,” EMBO Rep., 3, 224–229 (2002).
Eckel, B., Fasquelle, L., Moulin, M., et al., “Knock down of heat shock protein 27 (HspBl) induces degradation of several putative client proteins,” PLoS One, 7, No. 1, 297–319 (2012).
Friedman, M., Li, S., and Li, X.-J., “Activation of gene transcription by heat shock protein 27 may contribute to its neuronal protection,” J. Biol. Chem., 9, 27,944–27,951 (2009).
George, V. T., Brooks, G., and Humphrey, T. C., “Regulation of cell cycle and stress responses to hydrostatic pressure in fission yeast,” Mol. Biol. Cell., 18, No. 10, 4168–4179 (2007).
Grigor’yan, G. A. and Gulyaeva, N. V., “Stress reactivity and stress resistance in the pathogenesis of depressive disorders: the role of epigenetic mechanisms,” Zh. Vyssh. Nerv. Deyat., 65, No. 1, 19–32 (2015).
Kaminskaya, A. N., Nikitina, E. A., Payalina, T. L., et al., “Effects of the ratio of LIMK1 isoforms on mating behavior in Drosophila melanogaster: a complex approach,” Ekolog. Genetika, 9, No. 4, 3–14 (2011).
Kamyishev, N. G., Iliadi, K. G., Bragina, Yu. V., et al., “Detection of Drosophila mutants with memory defects after acquisition of conditioned reflex mating suppression,” Ros. Fiziol. Zh., 85, No. 1, 84–92 (1999).
Kamyshev, N. G., Iliadi, K. G., and Bragina, J. V., “Drosophila conditioned courtship: Two ways of testing memory,” Learn. Mem., 6, No. 1, 1–20 (1999).
Kapoor, P. and Shen, X., “Mechanisms of nuclear actin in chromatin remodeling complexes,” Trends Cell Biol., 24, No. 4, 238–246 (2014).
Kholodov, Yu. A. and Lebedeva, N. N., Reactions of the Nervous System to Electromagnetic Fields, Nauka, Moscow (1992).
Kourmouli, N., Jeppesen R, Mahadevhaiah, S., et al., “Heterochromatin and tri-methylated lysine 20 of histone H4 in animals,” J. Cell Sci., 117, 2491–2501 (2004).
Kuznetsov, P. A., Farmakovskii, B. V., Askinazi, A. Yu., et al., Patent No. 2324989 RF, “A composite material for protection against electromagnetic irradiation” (2006).
Landry, C. D., Kandel, E. R., and Rajasethupathy, P., “New mechanisms in memory storage: piRNAs and epigenetics,” Trends Neurosci., 36, No. 9, 535–542 (2013).
Lednev, V. V., Belova, N. A., Ermakov, A. M., et al., “Regulation of heart rate variability in humans using extremely weak varying magnetic fields,” Biofizika, 53, 648–654 (2008).
Li, J., Tan, M., Li, L., et al., “SAK, a new polo-like kinase, is transcriptionally repressed by p53 and induces apoptosis upon RNAi silencing,” Neoplasia, 7, No. 4, 312–323 (2005).
Maloney, M. T. and Bamburg, J. R., “Cofilin-mediated neurodegeneration in Alzheimer’s disease and other amyloidopathies,” Mol. Neurobiol., 35, No. 1, 21–44 (2007).
Masliah, E., “The role of synaptic proteins in Alzheimer’s disease,” Ann. N. Y. Acad. Sci., 924, 68–75 (2000).
Medvedeva, A. V., Molotkov, D. A., Nikitina, E. A., et al., “A system regulating genetic and cytogenetic processes by the actin remodeling signal cascade: the agnostic locus of Drosophila,” Genetika, 44, No. 6, 669–681 (2008).
Minamide, L. S., Striegl, A. M., Boyle, J. A., et al., “Neurodegenerative stimuli induce persistent ADF/cofilin-actin rods that disrupt distal neurite function,” Nat. Cell Biol., 2, 628–636 (2000).
Munsie, L., Caron, N., Atwal, R. S., et al., “Mutant huntingtin causes defective actin remodeling during stress: defining a new role for transglutaminase 2 in neurodegenerative disease,” Hum. Mol. Genet., 20, No. 10, 1937–1951 (2011).
Nikitina, E. A., Medvedeva, A. V., Dolgaya, Yu. F., et al., “The contribution of nucleosome-free regions of the LIMK1 gene in the spatial organization of the nucleus in polymorphic variants of the agnostic locus in Drosophila – models of human genomic diseases,” in: Health – the Basis of Human Potential: Problems and Ways of Solving them (2013), Vol. 8, No. 2, pp. 97–982.
Rath, U., Ding, Y., Deng, H., et al., “The chromodomain protein, Chromator, interacts with JIL-1 kinase and regulates the structure of Drosophila polytene chromosomes,” J. Cell Sci., 119, 2332–2341 (2006).
Rosso, S., Bollati, F., Bisbal, M., et al., “LIMK1 regulates Golgi dynamics, traffic of Golgi-derived vesicles, and process extension in primary cultured neurons,” Mol. Biol. Cell., 15, No. 7, 3433–3449 (2004).
Saito, A., Miyajima K, Akatsuka, J., et al., “CaMKIIβ-mediated LIMkinase activation plays a crucial role in BDNF-induced neuritogenesis,” Genes Cells, 18, No. 7, 533–543 (2013).
SantosRosa, H., Schneider, R., Bannister, A. J., et al., “Active genes are tri-methylated at K4 of histone H3,” Nature, 419, 407–411 (2002).
Savvateeva-Popova, E. V., Nikitina, E. A., Kurochkina, M. S., et al., “Actions of a weakened geomagnetic field as a factor regulating sexual behavior in Drosophila melanogaster,” in: Proc. 4th Int. Congress “Weak and Superweak Fields and Irradiation in Biology and Medicine” (2012).
Scott, R. W. and Oison, M. F., “LIM kinases: function, regulation and association with human disease,” J. Mol. Med., 85, 555–568 (2007).
Sjölinder, M., Bjork, P., Soderberg, E., et al., “The growing pre-mRNA recruits actin and chromatin-modifying factors to transcriptionally active genes,” Genes Dev., 19, No. 16, 1871–1884 (2005).
Sokal, R. R. and Rohlf, F. J., Biometry: The Principles and Practice of Statistics in Biological Research, W. H. Freeman, New York (1995).
Stokin, G. B. and Goldstein, L. S., “Axonal transport and Alzheimer’s disease,” Annu. Rev. Biochem., 75, 607–627 (2006).
Swaminathan, J., Baxter, E., and Corces, V., “The role of histone H2Av variant replacement and histone H4 acetylation in the establishment of Drosophila heterochromatin,” Genes Dev., 19, 65–76 (2005).
Turner, B. M., “Cellular memory and the histone code,” Cell, 111, 285–291 (2002).
Vatolina, Yu. T., Boldyreva, L. V., Demakova, O. V., et al., “Identical functional organization of nonpolytene and polytene chromosomes in Drosophila melanogaster,” PLoS One, 6, No. 10, 259–260 (2011).
Visa, N. and Percipalle, P. “Nuclear functions of actin,” Cold Spring Harb. Perspect. Biol., 2, No. 4, 620–634 (2010).
Vogel-Ciernia, A. and Wood, M. A., “Neuron-specific chromatin remodeling: A missing link in epigenetic mechanisms underlying synaptic plasticity, memory, and intellectual disability disorders,” Neuropharmacology, 80, 18–27 (2014).
World Health Organization, International Agency for Research on Cancer (IARC), “Non-ionizing radiation, Part I: static and extremely low-frequency (ELF) electric and magnetic fields,” IARC Monogr. Evaluat. Carcinog. Risks to Humans, 80, 1–395 (2002).
Zakharov, G. A., Molecular Genetic Studies of the Role of the Components of the Actin Remodeling Signal Cascade in the Genesis of Behavioral Disorders in Drosophila Melanogaster: Auth. Abstr. Mast. Thesis in Biol. Sci., St. Petersburg (2012).
Zegerman, P., Canas, B., Pappin, D., and Kouzarides, T., “Histone H3 lysine 4 methylation disrupts binding of nucleosome remodeling and deacetylase (NuRD) repressor complex,” J. Biol. Chem., 277, 11621–11624 (2002).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Zhurnal Vysshei Nervnoi Deyatel’nosti imeni I. P. Pavlova, Vol. 67, No. 2, pp. 246–256, March–April, 2017.
Rights and permissions
About this article
Cite this article
Nikitina, E.A., Medvedeva, A.V., Gerasimenko, M.S. et al. A Weakened Geomagnetic Field: Effects on Genomic Transcriptiln Activity, Learning, and Memory in Drosophila Melanogaster. Neurosci Behav Physi 48, 796–803 (2018). https://doi.org/10.1007/s11055-018-0632-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11055-018-0632-2