Autoprobiotic (indigenous) strains of Enterococcus faecium used in the correction of experimental intestinal dysbiosis, in contrast to probiotic E. faecium strain L-3, had a marked bifidogenic effect, preserved the populations of Escherichia, and inhibited the growth of Proteus, but had a relatively low antagonistic activity in relation to Klebsiella. Administration of autoprobiotics (A) and probiotic (P) led to faster disappearance of the symptoms of dyspepsia as compared with the control group of rats (C1) in which dysbiosis was not corrected. Animals from subgroup A1 were given A, whose genomes contained a large number of pathogenicity genes, including cytolysins and hyaluronidase. Investigations using the open field test identified various behavioral reactions to correction of dysbiosis. Animals of subgroup A1 showed suppression of movement and orientational-investigative activity. In the second group of rats (subgroup A2), movement and orientational-investigative activity was comparable with that in control group C2 (without induction of dysbiosis), as after use of P. These characteristics of the effects of autoprobiotic enterococci on the intestinal microbiota and the body support the existence of an intestinal microbiome–brain axis.
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References
I. N. Abdurasulova, A. F. Safonova, V. V. Sizov, et al., “Comparison of the actions of different probiotics on the motor and orientational-exploratory activity in rats,” in: Proc. 10th All-Russ. Sci. Appl. Conf. (2015), Vol 10, Pt. 1, pp. 1047–1051.
I. N. Abdurasulova, E. A. Tarasova, E. I. Ermolenko, et al., “Multiple sclerosis involves changes in the qualitative and quantitative composition of the intestinal microbiota,” Med. Akad. Zh., 15, No. 3, 55–67 (2015).
O. V. Averina, A. Yu. Ratushnyi, E. A. Tarasova, et al., “Effects of probiotics on cytokine production in in vitro and in vivo systems,” Med. Immunol., 17, No. 5, 443–454 (2015).
V. M. Bondarenko and A. A. Vorob’ev, “Dysbiosis and preparations with probiotic functions,” Zh. Mikrobiol., No. 1, 84–92 (2004).
A. E. Vershinin, V. V. Kolodzhieva, E. I. Ermolenko, et al., “Genetic identification as a method for identifying pathogenic and symbiotic strains of enterococci,” Zh. Mikrobiol., No. 5, 83–87 (2008).
L. M. Gunina, “Mechanisms of the effects of the probiotic ‘Laminolact Sportive’ on measures of specialist training of expert sportsmen,” in: Pedagogics, Psychology, and Medical-Biological Problems of Physical Nourishment and Sport (2012), Vol. 4, pp. 36–43.
I. V. Darmov, I. Yu. Chicherin, I. P. Pogorel’skii, et al., “Survival of probiotic microorganisms in the gastrointestinal tract of experimental animals,” Zh. Infektol., 4, No. 1, 68–74 (2012).
E. I. Ermolenko, Lactobacilli, Lambert Academic Publishing (2011).
E. I. Ermolenko, V. N. Donets, Yu. V. Dmitrieva, et al., “Effects of probiotic enterococci on the functional characteristics of the intestine in rats with antibiotic-induced dysbiosis,” Vestn. St. Peter. Univ. Med. Ser., 11, No. 1, 157–167 (2009).
E. I. Ermolenko, D. A. Seirido, M. L. Kotyleva, et al., “Correction of intestinal dysbiosis in rats with indigenous recombinant strains of enterococci and the duration of their persistence in the intestinal microbiocenosis,” Eksperim. Klin. Gastroenterol., 12, 10–25 (2016).
V. K. Il’in, A. N. Suvorov, N. V. Kiryukhina, et al., “Autoprobiotics for the prophylaxis of infectious-inflammatory diseases in humans in an artificial habitat,” Vestn. Ros. Akad. Med. Nauk., No. 2, 56–62 (2013).
M. A. Kirilenko and O. Yu. Kuznetsov, “Creation of an autoprobiotic formulation containing an active complex of bifidobacteria and lactobacteria,” Mezhdunar. Nauch.-Issl. Zh., 10, No. 41, 61–62 (2015).
E. I. Krasnova, N. I. Choklova, A. V. Vasinin, and I. A. Tsvetkova, “Acute intestinal infections: current aspects of the etiopathogenesis and the place of probiotics in treatment,” Pediatriya, No. 3, 80–84 (2015).
V. M. L’nyavina, Yu. P. Uspenskii, G. A. Alekhina, and A. N. Suvorov, “Use of probiotics as a factor in the correction of lipid metabolism in ischemic heart disease,” in: Innovatory Technologies for Controlling Health and Longevity in Humans: Proc. 1st Int. Sci. Appl. Conf., St. Petersburg (2010), pp. 24–28.
V. I. Simanenkhov, A. N. Suvorov, O. I. Solov’eva, et al., Patent 2546253 RF, “A means of obtaining a personalized autoprobiotic product and a means of treating irritable bowel syndrome using this product,” subm. April 25, 2013, reg. in RF State Register of Inventions March 2, 2015.
A. P. Khachatryan and R. G. Khachatryan, Patent 2126043 RF, C12N1/10, A61 35/74, “A means of obtaining a bank of autostrains of microorganisms for restoring the intestinal microbiocenosis in humans,” subm. Feb. 10, 1999.
P. V. Seliverstov, V. G. Radchenko, I. G. Safronenkova, and S. I. Sitkin, “Interaction of the liver and intestine on the background of imbalance in the large intestinal microflora,” Gastroenterol. St-Peter., No. 2–3, 15–18 (2010).
B. A. Shenderov and M. A. Manvelova, Auth. Certif. No. 1286212 USSR, A 61 K 35/74, “A means of obtaining an autoprobiotic containing live bifidobacteria and lactobacilli,” subm. Jan. 30, 1987.
I. Yu. Chicherin, I. P. Pogorel’skii, I. A. Lundovskikh, et al., “Autoprobiotic therapy,” Zh. Infektol., 5, No. 4, 43–54 (2013).
E. Barrett, R. P. Ross, P. W. O’Toole, et al., “Gamma-aminobutyric acid production by culturable bacteria from the human intestine,” J. Appl. Microbiol., 113, No. 2, 411–417 (2012).
P. Bercik, E. F. Verdu, J. A. Foster, et al., “Chronic gastrointestinal inflammation induces anxiety-like behavior and alters central nervous system biochemistry in mice,” Gastroenterology, 139, No. 6, 2102–2112 (2010).
P. Bercik, E. Denou, J. Collins, et al., “The intestinal microbiota affect central levels of brain-derived neurotropic factor and behavior in mice,” Gastroenterology, 141, No. 2, 599–609 (2011).
P. Bercik, A. J. Park, D. Sinclair, et al., “The anxiolytic effect of Bifidobacterium longum NCC3001 involves vagal pathways for gutbrain communication,” Neurogastroenterol. Motil., 23, No. 12, 1132–1139 (2011).
J. A. Bravo, P. Forsythe, M. V. Chew, et al., “Ingestion of Lactobacillus strain regulates emotional behavior and central GABA receptor expression in a mouse via the vagus nerve,” Proc. Natl. Acad. Sci. USA, 108, No. 38, 16050–16055 (2011).
F. Brouns and E. Beckers, “Is the gut an athletic organ? Digestion, absorption and exercise,” Sports Med., 15, No. 4, 242–257 (1993).
S. M. Collins, M. Surette, and P. Bercik, “The interplay between the intestinal microbiota and the brain,” Nat. Rev. Microbiol., 10, No. 11, 735–742 (2012).
S. Davari, S. A. Talaei, H. Alaei, and M. Salami, “Probiotics treatment improves diabetes-induced impairment of synaptic activity and cognitive function: behavioral and electrophysiological proofs for microbiome-gut-brain axis,” Neuroscience, 240, 287–296 (2013).
E. Ermolenko, L. Gromova, Yu. Borschev, et al., “Influence of different probiotic lactic acid bacteria on microbiota and metabolism of rats with dysbiosis,” Biosci. Microbiota Food Health, 32, No. 2, 41–49 (2013).
S. M. Finegold, S. E. Dowd, V. Gontcharova, et al., “Pyrosequencing study of fecal microflora of autistic and control children,” Anaerobe, 16, No. 4, 444–453 (2010).
M. G. Gareau, E. Wine, D. M. Rodrigues, et al., “Bacterial infection causes stress-induced memory dysfunction in mice,” Gut, 60, No. 3, 307–317 (2011).
R. D. Heijtz, S. Wang, F. Anuar, et al., “Normal gut microbiota modulates brain development and behavior,” Proc. Natl. Acad. Sci. USA, 108, No. 7, 3047–3052 (2011).
C. Jernberg, S. Lofmark, C. Edlund, and J. K. Jansson, “Long-term impacts of antibiotic exposure on the human intestinal microbiota,” Microbiology, 156, No. 11, 3216–3223 (2010).
H. Jiang, Z. Ling, Y. Zhang, et al., “Altered fecal microbiota composition in patients with major depressive disorder,” Brain Behav. Immun., 48, 186–194 (2015).
A. Karaseva, A. Tsapieva, J. Pachebat, and A. Suvorov, “Draft genome sequence of probiotic Enterococcus faecium strain L-3,” Genome Announc., 4, No. 1, e01622-15 (2016).
L. V. McFarland, “Use of probiotics to correct dysbiosis of normal microbiota following disease or disruptive events: a systematic review,” BMJ Open, 4, No. 8, e005047 (2014).
M. Messaoudi, R. Lalonde, N. Violle, et al., “Assessment of psychotropic-like properties of a probiotic formulation (Lactobacillus helveticus R0052 and Bifidobacterium longum R0175) in rats and human subjects,” Brit. J. Nutr., 105, No. 5, 755–764 (2011).
M. I. Naseer, F. Bibi, M. H. Alqahtani, et al., “Role of gut microbiota in obesity, type 2 diabetes and Alzheimer’s disease,” CNS Neurol. Disord. Drug Targets, 13, No. 2, 305–311 (2014).
W. Ochmański and W. Barabasz, “Probiotics and their therapeutic properties at sportsmen,” Rev. Przegl. Lek., 56, No. 3, 211–215 (1999).
I. Sekirov, S. L. Russell, L. C. Antunes, and B. B. Finlay, “Gut microbiota in health and disease,” Physiol. Rev., 90, No. 3, 859–904 (2010).
A. Suvorov, “Gut microbiota, probiotics, and human health,” Biosci. Microbiota Food Health, 32, No. 3, 81–91 (2013).
E. Tarasova, E. Yermolenko, V. Donets, et al., “The influence of probiotic enterococci on the microbiota and cytokines expression in rats with dysbiosis induced by antibiotics,” Benef. Microbes, 1, No. 3, 265–270 (2010).
K. Tillisch, J. Labus, L. Kilpatrick, et al., “Consumption of fermented milk product with probiotic modulates brain activity,” Gastroenterology, 144, No. 7, 1394–1401 (2013).
H. A. Tilson and C. L. Mitchell, “Neurobehavioral techniques to assess the effects of chemicals on the nervous system,” Annu. Rev. Pharmacol. Toxicol., 24, 425–450 (1984).
B. L. Williams, M. Hornig, T. Parekh, and W. I. Lipkin, “Application of novel PCR-based methods for detection, quantitation, and phylogenetic characterization of Sutterella species in intestinal biopsy samples from children with autism and gastrointestinal disturbances,” Mbio, 3, No. 1 (2012), pii: e00261-11, doi: https://doi.org/10.1128/mBio.00261-11.
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Translated from Rossiiskii Fiziologicheskii Zhurnal imeni I. M. Sechenova, Vol. 103, No. 1, pp. 22–37, January, 2017.
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Ermolenko, E.I., Abdurasulova, I.N., Kotyleva, M.P. et al. Effects of Indigenous Enterococci on the Intestinal Microbiota and the Behavior of Rats on Correction of Experimental Dysbiosis. Neurosci Behav Physi 48, 496–505 (2018). https://doi.org/10.1007/s11055-018-0591-7
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DOI: https://doi.org/10.1007/s11055-018-0591-7