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A Method of Enhanced Immunofluorescence by Amplification of the Immunoperoxidase Reaction

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The aim of the present work was to develop a simple and effective means of converting the immunoperoxidase reaction into an immunofluorescence reaction. Use of fluorochrome-conjugated antibodies to horseradish peroxidase significantly increased the intensity of the immunofluorescence reaction as compared with the indirect Coons method, which was confirmed by quantitative evaluation of fluorescence intensity. The conversion method developed here does not require the protocol to be expanded by a large number of steps or any reagent substitutions, which facilitates the transition from the immunoperoxidase reaction to the immunofluorescent reaction.

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  1. V. V. Gusel’nikova, S. A. Bekoeva, V. F. Korzhevskaya, et al., “Histochemical and immunohistochemical identification of mast cells in the human myocardium,” Morfologiya, 147, No. 2, 80–86 (2015).

    Google Scholar 

  2. D. E. Korzhevskii, O. V. Kirik, M. N. Karpenko, et al., Theoretical Bases and Applied Use of Immunohistochemical Methods: Guidelines, SpetsLit, St. Petersburg (2012).

    Google Scholar 

  3. D. E. Korzhevskii (ed.), O. V. Kirik, E. S. Petrova, et al., Theoretical Bases and Applied Use of Immunohistochemical Methods: Guidelines, SpetsLit, St. Petersburg (2014), 2nd ed.

  4. D. E. Korzhevskii, O. V. Kirik, E. G. Sukhorukova, et al., Molecular Morphology. Methods of Fluorescence and Confocal Laser Microscopy, SpetsLit, St. Petersburg (2014).

  5. D. E. Korzhevskii, E. G. Sukhorukova, E. G. Gilerovich, et al., “Advantages and disadvantages of zinc-ethanol-formaldehyde as a fixer for immunocytochemical studies and confocal laser microscopy,” Morfologiya, 143, No. 2, 81–85 (2013).

    CAS  Google Scholar 

  6. E. G. Sukhorukova, “Nuclear protein NeuN in human substantia nigra neurons,” Morfologiya, 143, No. 2, 78–80 (2013).

    CAS  Google Scholar 

  7. E. G. Sukhorukova, O. V. Kirik, N. M. Zelenkova, and D. E. Korzhev skii, “Neuronal nuclear antigen NeuN – an indicator of the preservation of neural tissue and its suitability for immunohistochemical studies,” Med. Akad. Zh., No. 1, 63–67 (2015).

  8. O. S. Alekseeva, V. V. Gusel’nikova, G. V. Beznin, D. E. Korzhevskii, “Prospects of the nuclear protein NeuN application as an index of functional state of the vertebrate nerve cells,” Zh. Evol. Biokhim. Fiziol., 51, No. 5, 313–323 (2015).

    CAS  PubMed  Google Scholar 

  9. V. V. Gusel’nikova and D. E. Korzhevskii, “NeuN as a neuronal nuclear antigen and neuron differentiation marker,” Acta Naturae, 7, No. 2, 42–47 (2015).

    PubMed  PubMed Central  Google Scholar 

  10. T. Ibaraki, M. Muramatsu, S. Takai, et al., “The relationship of tryptase-and chymase-positive mast cells to angiogenesis in stage I nonsmall cell lung cancer,” Eur. J. Cardiothorac. Surg., 28, No. 4, 617–621 (2005).

    Article  PubMed  Google Scholar 

  11. G. Ranieri, M. Ammendola, R. Patruno, et al., “Tryptase-positive mast cells correlate with angiogenesis in early breast cancer patients,” Int. J. Oncol., 35, No. 1, 115–120 (2009).

    Article  PubMed  Google Scholar 

  12. J. Vitte, “Human mast cell tryptase in biology and medicine,” Mol. Immunol., 63, No. 1, 18–24 (2015).

    Article  CAS  PubMed  Google Scholar 

  13. G. Wang, C. L. Achim, R. L. Hamilton, et al., “Tyramide signal amplification method in multiple-label immunofluorescence confocal microscopy,” Methods, 18, No. 4, 459–464 (1999).

    Article  CAS  PubMed  Google Scholar 

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Correspondence to V. V. Gusel’nikova.

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Translated from Morfologiya, Vol. 151, No. 1, pp. 91–94, January–February, 2017.

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Gusel’nikova, V.V., Korzhevskii, D.E. A Method of Enhanced Immunofluorescence by Amplification of the Immunoperoxidase Reaction. Neurosci Behav Physi 48, 153–156 (2018).

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