A Method of Enhanced Immunofluorescence by Amplification of the Immunoperoxidase Reaction
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The aim of the present work was to develop a simple and effective means of converting the immunoperoxidase reaction into an immunofluorescence reaction. Use of fluorochrome-conjugated antibodies to horseradish peroxidase significantly increased the intensity of the immunofluorescence reaction as compared with the indirect Coons method, which was confirmed by quantitative evaluation of fluorescence intensity. The conversion method developed here does not require the protocol to be expanded by a large number of steps or any reagent substitutions, which facilitates the transition from the immunoperoxidase reaction to the immunofluorescent reaction.
Keywordsimmunoperoxidase reaction immunofluorescence reaction conversion amplification
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