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Features of the Action of Combined Administration of NAN-190 and Ketanserin with Low-Dose 17β-Estradiol on Depression-Like Behavior in Prenatally Stressed Rats

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The aim of the present work was to compare the effects of blockade of 5-HT1A or 5-HT2A/2C serotonin receptors on depression-like behavior in adult female rats with experimental estrogen deficiency whose mothers had been subjected to prenatal stress during pregnancy. Two weeks after removal of the ovaries, ovariectomized (OE) prenatally stressed female rats received chronic administration of the 5-HT1A serotonin receptor antagonist NAN-190 (0.1 mg/kg, i.p.) or the 5-HT2A/2C serotonin receptor antagonist ketanserin (0.1 mg/kg, i.p.) alone or in combination with a low dose of 17β-estradiol (0.5 μg/rat, s.c.) for 14 days before behavior tests started and then until tests were completed. The behavioral methods used were the Porsolt test and the open field test. The results showed that chronic administration of NAN-190 to OE prenatally stressed females led to a prodepressive effect, while the combination of NAN-190 with low-dose 17β-estradiol, conversely, had an antidepressant effect in the Porsolt test. In the open field test, these two experimental groups of rats showed decreases in the amounts of grooming reactions, vertical motor activity, and exploratory activity. Administration of ketanserin to OE prenatally stressed females produced an antidepressant effect, as compared with control animals. However, combined administration of ketanserin and low-dose 17β-estradiol to OE prenatally stressed females had no positive effect on the behavior of the animals in the Porsolt test.

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References

  1. S. V. Barabanova, Yu. O. Fedotova, and N. S. Sapronov, “Effects of antagonists of the 2A and 2A/2C subtypes of serotonin receptors on depressive behavior and C-FOS protein expression in the hypothalamus in ovariectomized rats,” Eksperim. Klin. Farmakol., 70, No. 2, 3–7 (2007).

    CAS  Google Scholar 

  2. Ya. Buresh, O. Bureshova, and J. P. Houston, Methods and Basic Experiments in Studies of the Brain and Behavior, Vysshaya Shkola, Moscow (1991).

    Google Scholar 

  3. Ya. D. Kirshenblat, Handbook of Endocrinology, Vysshaya Shkola, Moscow (1969).

    Google Scholar 

  4. N. E. Ordyan and S. G. Pivina, “Characteristics of behavior and the stress reactivity of the hypophyseal-adrenal system in prenatally stressed rats,” Ros. Fiziol. Zh., 89, No. 1, 52–59 (2003).

    Google Scholar 

  5. N. S. Sapronov and Yu. O. Fedotova, “Estrogens and psychoneurological status in in women,” Med. Akad. Zh., 7, No. 2, 24–34 (2007).

    Google Scholar 

  6. Yu. O. Fedotova, “Neurohormonal mechanisms of the antidepressant action of ketanserin in ovariectomized rats,” Neirokhimiya, 27, No. 4, 1–7 (2010).

    Google Scholar 

  7. C. L. Bethea, N. Z. Lu, C. Gundlan, and J. M. Streicker, “Diverse actions of ovarian steroids in the serotonin neural system,” Front. Neuroendocrinol., 23, 41–100 (2002).

    Article  CAS  PubMed  Google Scholar 

  8. V. Harsh, S. Meltzer-Brody, D. R. Rubinow, and P. J. Schmidt, “Reproductive aging, sex steroids, and mood disorders,” Harv. Rev. Psychiatry, 17, 87–102 (2009).

    Article  PubMed  PubMed Central  Google Scholar 

  9. A. Markou, T. Duka, and G. M. Prelevic, “Estrogens and brain function,” Hormones (Athens), 4, 9–17 (2005).

    Article  Google Scholar 

  10. K. Miyagawa, M. Tsuji, K. Fujimori, et al., “Prenatal stress induces anxiety-like behavior together with the disruption of central serotonin neurons in mice,” Neurosci. Res., 70, 111–117 (2011).

    Article  CAS  PubMed  Google Scholar 

  11. R. E. Noble, “Depression in women,” Metabolism, 54, 49–52 (2005).

    Article  CAS  PubMed  Google Scholar 

  12. J. P. Olie, E. Costa, J. Silva, and J. P. Macher, Neuroplasticity: a New Approach to the Pathophysiology of Depression, Science Press, London (2004).

    Google Scholar 

  13. R. D. Porsolt, A. Bertin, and M. Jalfre, “Behavioral despair in rats and mice: strain differences and the effects of imipramine,” Eur. J. Pharmacol., 51, 291–294 (1978).

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia

    Yu. O. Fedotova, S. G. Pivina, V. K. Akulova & N. E. Ordyan

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  1. Yu. O. Fedotova
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  2. S. G. Pivina
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  3. V. K. Akulova
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  4. N. E. Ordyan
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Correspondence to Yu. O. Fedotova.

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Translated from Rossiiskii Fiziologicheskii Zhurnal imeni I. M. Sechenova, Vol. 101, No. 1, pp. 35–43, January, 2015.

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Fedotova, Y.O., Pivina, S.G., Akulova, V.K. et al. Features of the Action of Combined Administration of NAN-190 and Ketanserin with Low-Dose 17β-Estradiol on Depression-Like Behavior in Prenatally Stressed Rats. Neurosci Behav Physi 46, 571–575 (2016). https://doi.org/10.1007/s11055-016-0279-9

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  • DOI: https://doi.org/10.1007/s11055-016-0279-9

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