Experiments were performed on four C57black/6 mice to study neurons containing calbindin protein (CB) with a molecular weight of 28 kD and neurofi laments (NF) with a molecular weight of 200 kD in the ventral horn of the gray matter of spinal cord (SC) segments TII, LIV, LV, and LVI. Immunopositive neurons were detected by labeling with antibodies against CB and double labeling with antibodies against CB and NF. The whole cell population was labeled with NeuroTrace Red Fluorescent Nissl stain. The results of these studies showed that CB-immunopositive (CB+) neurons detected in the ventromedial area of the ventral horn at all levels of the SC were Renshaw cells. CB+ interneurons located in the medial part of the ventral horn were present only in the lumbar segments of the SC. CB+ motoneurons detected in the medial part of the ventral horn were present in one SC segment – LIV – and also contained NF protein.
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Translated from Morfologiya, Vol. 146, No. 4, pp. 21–25, July–August, 2014.
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Porseva, V.V., Shilkin, V.V., Strelkov, A.A. et al. Calbindin-Containing Neurons in the Ventral Horn of the Gray Matter of the Spinal Cord in Mice. Neurosci Behav Physi 45, 710–714 (2015). https://doi.org/10.1007/s11055-015-0133-5
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DOI: https://doi.org/10.1007/s11055-015-0133-5