The effects of late prenatal stress on free-radical oxidation of proteins in Sprague–Dawley rats were studied in the cerebral cortex, striatum, hippocampus, and hypothalamus, along with changes in behavior in test systems involving different levels of stress induction. The results of these experiments showed that the level of spontaneous oxidation in the hypothalamus of rats aged 100 days was increased, while induced oxidation was decreased relative to the control group; the opposite effects were seen in the hippocampus. The cerebral cortex and striatum showed minor changes in study parameters. In the stress-inducing open field behavioral test, prenatally stressed animals aged 100 days showed reduced motor activity and increased freezing and grooming times. Conversely, motor activity in the T maze and grooming time in prenatally stressed animals increased compared with controls, while freezing decreased. It is suggested that the improvements in behavioral measures in the T maze in prenatally stressed animals are associated with persistence of oxidative modification processes in the cortex and some improvement in measures in the hippocampus. Adverse changes in protein oxidation processes, particularly in the hippocampus, were reflected in the responses of prenatally stressed animals to the stress-inducing conditions of the open field test.
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References
E. E. Dubinina, S. O. Burmistrov, D. A. Khodov, and I. E. Porotov, “Oxidative modification of serum proteins in humans,” Vopr. Med. Khim., 41, No. 1, 24–26 (1995).
E. E. Dubinina, Products of Oxygen Metabolism in the Functional Activity of Cells [in Russian], Med. Press, St. Petersburg (2006).
A. A. Garibyan, The Role of Deep Brain Structures in the Mechanisms of Directed Behavior [in Russian], Meditsina, Moscow (1984).
D. I. Kuz’menko and B. I. Laptev, “Assessment of serum lipid reserves for peroxidation during oxidative stress in rats,” Vopr. Med. Khim., 45, No. 1, 47–54 (1999).
A. L. Markel’, “Evaluation of basic behavioral characteristics of rats in the ‘open field’ test,” Zh. Vyssh. Nerv. Deyat., 31, No. 2, 301–307 (1981).
I. N. Nazarov, L. A. Kazitsina, and I. I. Zaretskaya, “Studies of the absorption spectra of 2,4-dinitrophenylhydrazones of carbonyl compounds,” Zh. Obshch. Khim., 27, No. 3, 606–623 (1956).
S. N. Olenev, The Developing Brain (cellular, molecular, and genetic aspects of neuroembryology) [in Russian], Nauka, Leningrad (1978).
N. E. Ordyan and S. G. Pivina, “Characteristics of the behavior and stress reactivity of the hypophyseal-adrenocortical system in prenatally stressed rats,” Ros. Fiziol. Zh., 84, No. 1, 52–59 (2003).
A. V. Peskin, “Interaction of active oxygen with DNA,” Biokhimiya, 62, No. 12, 1571–1578 (1997).
V. N. Semagin and V. V. Antipov, Compensatory Potentials of Nervous Activity on Irradiation of Embryos [in Russian], Nauka, Moscow (1978).
V. V. Sokolovskii,V. S. Kuz’mina, G. A. Moskadynova, and N. N. Petrova, “Spectrophotometric assay of thiols of serum,” Klin. Lab. Diagnost., 11, 20–21 (1997).
M. A. Flerov, I. A. Gerasimova, and A. V. V’yushina, “Effects of prenatal stress on lipid peroxidation in various parts of the brains of male and female adult rats,” Neirokhimiya, 22, No. 3, 1–6 (2005).
S. Chavarin, I. Chaba, and I. Sekei, “The role of superoxide dismutase in oxidative processes in cells and methods for its assay in biological materials,” Lab. Delo, 11, 678–681 (1985).
V. G. Shalyapina, I. N. Zaichenko, A. S. Batuev, and N. E. Ordyan, “Changes in the neuroendocrine regulation of adaptive behavior after stress and late prenatal ontogenesis,” Ros. Fiziol. Zh., 87, 1193–1201 (2001).
M. A. Berger, V. G. Barros, M. I. Sarchi, et al., “Long-term effects of prenatal stress on dopamine and glutamate receptors in adult rat brain,” Neurochem. Res., 27, No. 11, 1525–1533 (2002).
R. H. Burdon, “Superoxide and hydrogen peroxide in relation to mammalian cell proliferation,” Free Rad. Biol. Med., 18, No. 4, 775–794 (1995).
E. Charmandari, T. Kino, E. Souvatzoglou, and G. P. Chrousos, “Pediatric stress: hormonal mediators and human development,” Horm. Res., 59, No. 4, 161–179 (2003).
B. Halliwell, “Free radicals, proteins and DNA oxidative damage versus redox regulation,” Biochem. Soc. Trans., 24, No. 4, 1023–1027 (1996).
A. K. Kinnunen, J. I. Koenig, and G. Bilbe, “Repeated variable prenatal stress alters pre- and postsynaptic gene expression in the rat frontal pole,” J. Neurochem., 86, No. 3 736–748 (2003).
L. Y. Rizhova and E. A. Vershinina, “The dynamics of two different tests of laterality in rats,” Laterality, 5, No. 4, 331–350 (2000).
M. Weinstock, “Can the behavior abnormalities induced by gestational stress in rats be prevented or reversed?” Stress, 5, No. 3, 167–176 (2002).
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Translated from Rossiiskii Fiziologicheskii Zhurnal imeni I. M. Sechenova, Vol. 98, No. 8, pp. 962–969, August, 2012.
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V’yushina, A.V., Pritvorova, A.V. & Flerov, M.A. Oxidative Modification of Proteins in Brain Structures in Sprague–Dawley Rats and Some Behavioral Parameters after Prenatal Stress. Neurosci Behav Physi 44, 395–400 (2014). https://doi.org/10.1007/s11055-014-9924-3
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DOI: https://doi.org/10.1007/s11055-014-9924-3