Our previous studies of the molecular and cellular mechanisms underlying the generation of rhythmic movements motor patterns were based on use of a selection of candidate genes in which mutations cause impairments to the motor activity of Drosophila melanogaster. We report here testing of the locomotor behavior of Drosophila strains with decreases in the expression of 12 candidate genes in the nervous system. Target genes were suppressed by synthesizing interfering RNA using the GAL4/UAS system under the control of the elav, nrv2, appl, and tsh gene promoters (drivers). These experiments showed that RNA interference of virtually all the candidate genes was accompanied by changes in one or more locomotor parameters. The nature of the abnormalities occurring under the control the various drivers allowed us to identify those genes whose activity in nervous system cells is required for the normal functioning of the central motor pattern generator for locomotor acts.
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References
N. G. Kamyshev, “Drosophila as a model system for discovering the molecular mechanisms of motor and cognitive dysfunctions and the search for pharmacological means for correcting them: a quantitative approach to assessing changes in locomotor behavior,” in: Measurement and Information Technologies in Healthcare: Coll. Sci. Works, St. Petersburg (2011), pp. 152–160.
S. A. Fedotov, Yu. V. Bragina, N. G. Besedina, et al., “Genetic studies of motor functions in Drosophila melanogaster,” Ekol. Genetika, 10, No. 1, 51–61 (2012).
S. Abdelilah-Seyfried, Y. M. Chan, C. Zeng, et al., “A gain-of-function screen for genes that affect the development of the Drosophila adult external sensory organ,” Genetics, 155, No. 2, 733–752 (2000).
Y. I. Arshavsky, T. G. Deliagina, and G. N. Orlovsky, “Pattern generation,” Curr. Opin. Neurobiol., 7, No. 6, 781–789 (1997).
C. Bagni, S. Bray, J. A. Gogos, et al., “The Drosophila zinc finger transcription factor CF2 is a myogenic marker downstream of MEF2 during muscle development,” Mech. Dev., 117, No. 1–2, 265–268 (2002).
K. L. Briggman and W. B. Kristan, “Multifunctional pattern-generating circuits,” Annu. Rev. Neurosci., 31, 271–294 (2008).
R. S. Burton and A. la Spada, “Trehalase polymorphism in Drosophila melanogaster,” Biochem. Genet., 24, No. 9–10, 715–719 (1986).
V. R. Chintapalli, J. Wang, and J. A. Dow, “Using FlyAtlas to identify better Drosophila models of human disease,” Nat. Genetics, 39, No. 6, 715–720 (2007).
P. Chomczynski and N. Sacchi, “The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on,” Nat. Protoc., 1, No. 2, 581–585 (2006).
J. B. Duffy, “GAL4 system in Drosophila: a fly geneticist’s Swiss army knife,” Genesis, 34, No. 1–2, 1–15 (2002).
E. S. Edgington, Randomization Tests Marcel Dekker, New York (1995), 3rd ed.
L. Fasano, L. Roder, N. Core, et al., “The gene teashirt is required for the development of Drosophila embryonic trunk segments and encodes a protein with widely spaced zinc finger motifs,” Cell, 64, No. 1, 63–79 (1991).
J. Kim, S. Lee, S. Ko, and J. Kim-Ha, “dGIPC is required for the locomotive activity and longevity in Drosophila,” Biochem. Biophys. Res. Commun., 402, No. 3, 565–570 (2010).
L. E. Martin-Morris and K. White, “The Drosophila transcript encoded by the beta-amyloid protein precursor-like gene is restricted to the nervous system,” Development, 110, No. 1, 185–195 (1990).
C. McCormick, G. Duncan, K. T. Goutsos, and F. Tufaro, “The putative tumor suppressors EXT1 and EXT2 form a stable complex that accumulates in the Golgi apparatus and catalyzes the synthesis of heparin sulfate,” Proc. Natl. Acad. Sci. USA, 97, No. 2, 668–673 (2000).
R. Muller, A. J. Hulsmeier, F. Altmann, et al., “Characterization of mucin-type core-1 β1-3 galactosyltransferase homologous enzymes in Drosophila melanogaster,” FEBS. J., 272, No. 17, 4295–4305 (2005).
J. Z. Parrish, M. D. Kim, L. Y. Jan, and Y. N. Jan, “Genome-wide analyses identify transcription factors required for proper morphogenesis of Drosophila sensory neuron dendrites,” Genes Dev., 20, No. 7, 820–835 (2000).
M. W. Pfaffl, G. W. Horgan, and L. Dempfle, “Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR,” Nucl. Acids Res., 30, No. 9, e36 (2002).
S. Robinow and K. White, “Characterization and spatial distribution of the ELAV protein during Drosophila melanogaster development,” J. Neurobiol., 22, No. 5, 443–461 (1991).
T. J. Sheldon, I. Miguel-Aliaga, A. P. Gould, et al., “A novel family of single VWC-domain proteins in invertebrates,” FEBS Lett., 581, No. 27, 5268–5274 (2007).
M. S. Shim, J. Y. Kim, H. K. Jung, et al., “Elevation of glutamine level by selenophosphate synthetase I knockdown induces metamitochondrial formation in Drosophila cells,” J. Biol. Chem., 284, No. 47, 32,881–32,894 (2009).
J. A. Simon and J. T. Lis, “A germline transformation analysis reveals flexibility in the organization of heat shock consensus elements,” Nucl. Acids Res., 15, No. 7, 2971–2988 (1987).
R. Strauss, “The central complex and the genetic dissociation of locomotor behaviour,” Curr. Opin. Neurobiol., 12, No. 6, 633–638 (2002).
B. Sun, P. Xu, and P. M. Salvaterra, “Dynamic visualization of nervous system in live Drosophila,” Proc. Natl. Acad. Sci. USA, 96, No. 18, 10,438–10,443 (1999).
W. Supatto, A. McMahon, S. E. Fraser, and A. Stathopoulos, “Quantitative imaging of collective migration during Drosophila gastrulation: multiphoton microscopy and computational analysis,” Nat. Protoc., 4, No. 10, 1397–1412 (2009).
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Translated from Rossiiskii Fiziologicheskii Zhurnal imeni I. M. Sechenova, Vol. 99, No. 1, pp. 120–130, January, 2013.
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Fedotov, S.A., Bragina, Y.V., Besedina, N.G. et al. Genetic Determinants of Rhythmic Movements Motor Pattern Generation in Drosophila Melanogaster . Neurosci Behav Physi 44, 995–1001 (2014). https://doi.org/10.1007/s11055-014-0015-2
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DOI: https://doi.org/10.1007/s11055-014-0015-2
Keywords
- locomotion
- Drosophila
- RNA interference
- central motor pattern generators