The effects of tenotomy present for two weeks on the post-tetanic responses of isolated fast (extensor digitorum longus) and slow (soleus) muscles were studied by isometric recording of muscle contraction in rats. After tetanic stimulation with bursts of 100 impulses at a frequency of 20 impulses/sec, the force of single fast muscle contractions exceeded the pretetanic level by 20–25% (p < 0.05) in both intact and tenotomized muscles. In similar conditions, the slow muscle soleus showed a decrease in the amplitudes of single contractions, i.e., post-tetanic depression. Post-tetanic depression was not seen after tenotomy. Caffeine (4 mM) increased the force of single and tetanic contractions in control and tenotomized fast muscles, though no corresponding increase in post-tetanic responses were seen. In slow muscles, caffeine had no effect on the nature of post-tetanic responses. Dantrolene (10 μM) decreased the force of single and tetanic contractions but did not prevent the appearance of post-tetanic potentiation in fast muscles and depression in intact slow muscles. The fact that the phenomenon of post-tetanic potentiation disappeared after denervation of fast muscles within two weeks while it was present after tenotomy for as long as 40 days after surgery provides grounds for suggesting the existence of a potentiating factor which reaches muscles by axonal transport and increases single contractions.
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References
R. S. Arutyunyan and S. V. Kuznetsov, “Effects of neurogenic inactivity on post-tetanic responses of fast muscles in rats,” Zh. Evolyuts. Biokhim. Fiziol., 46, No. 1, 66–73 (2010).
J. Bagust, D. Lewis, and J. Luck, “Post-tetanic effects in motor units of fast and slow twitch muscle of the cat,” J. Physiol. (London), 237, No. 1, 115–121 (1974).
J. Bagust, “The effects of tenotomy upon the contraction characteristics of motor units in the rabbit soleus muscle,” J. Physiol. (London), 200, No. 1, 1–10 (1979).
N. Beard, D. Lever, and A. Dulhunty, “Calsequestrin and calcium release channel of skeletal and cardiac muscle,” Progr. Biophys. Molec. Biol., 85, No. 1, 33–69 (2004).
A. Buller and D. Lewis, “Some observations on the effects of tenotomy in the rabbit,” J. Physiol. (London), 178, No. 2, 326–342 (1965).
A. Buller, C. Kean, K. Ranatunga, and M. Smith, “Post-tetanic depression of twitch tension in cat soleus muscle,” Exp. Neurol., 73, No. 1, 78–89 (1981).
R. Close and J. Hoh, “The after effect of repetitive stimulation on the isometric twitch contraction of rat fast skeletal muscle,” J. Physiol. (London), 197, No. 2, 461–477 (1968).
R. Close and J. Hoh, “Post-tetanic potentiation of twitch contraction of cross-innervated rat fast and slow muscles,” Nature, 221, No. 5176, 179–181 (1969).
M. Fryer and J. Neering, “Actions of caffeine on fast and slow twitch muscle,” J. Physiol. (London), 416, 435–454 (1989).
R. Jackman and S. Kandarian, “Molecular basis of skeletal muscle atrophy,” Am. J. Cell Physiol., 287, No. 3, 834–843 (2004).
A. Jakubiec-Puka, C. Catani, and U. Carraro, “Myosin heavy-chain composition in striated muscle after tenotomy,” Biochem. J., 282, No. 1, 237–242 (1992).
A. Jamali, P. Afshar, R. Abrams, and R. Lieber, “Skeletal muscle response to tenotomy,” Muscle Nerve, 23, No. 6, 851–862 (2000).
A. Hermann-Frank, H. Luttgau, and D. Stephenson, “Caffeine and excitation contraction coupling in skeletal muscle: a stimulatory story,” J. Muscle Res. Cell Motil., 20, No. 2, 223–237 (1999).
C. Krarup, “Enhancement and diminution of mechanical tension evoked by staircase and by tetanus in rat muscle,” J. Physiol. (London), 311, 355–372 (1981).
C. Krarup, “The effect of dantrolene on the enhancement and diminution of tension evoked by staircase and by tetanus in rat muscle,” J. Physiol. (London), 311, 389–400 (1981).
G. Lamb, M. Cellini, and D. Stephenson, “Different Ca2+ releasing action of caffeine and depolarization in skeletal fibres of the rat,” J. Physiol. (London), 531, No. 3, 715–728 (2001).
B. MacIntosh and P. Gardiner, “Post-tetanic potentiation and skeletal muscle fatigue: interaction with caffeine,” Can. J. Physiol. Pharmacol., 65, No. 2, 260–268 (1987).
R. McMinn and G. Vrbova, “The effect of tenotomy on the structure of fast and slow muscle of the rabbit,” Quart. J. Exp. Physiol., 49, No. 4, 424–430 (1964).
W. Melzer, A. Hermann-Frank, and H. Luttgau, “The role of Ca2+ ions in excitation-contraction coupling of skeletal muscle fibres,” Biochem. Biophys. Acta, 1241, No. 1, 59–116 (1995).
J. Mickelson and C. Louis, “Malignant hyperthermia: excitation-contraction coupling Ca2+ channel and cell Ca2+ regulation defect,” Physiol. Rev., 76, No. 2, 537–592 (1996).
P. G. Nelson, “Functional consequences of tenotomy in hind limb muscles of the cat,” J. Physiol. (London), 201, No. 2, 321–333 (1969).
B. Pachter and N. Spielholz, “Tenotomy induced motor end plate alterations in rat soleus muscle,” Anat. Rec., 228, No. 1, 104–108 (1990).
T. Radzyukevich and J. Heiny, “Regulation of dihydropyridine receptor gene expression in mouse skeletal muscles by stretch and disuse,” Am. J. Physiol. (Cell Physiol.), 287, No. 5, 1445–1452 (2004).
J. Resnick, W. Engel, and P. Nelson, “Changes in the Z disk of skeletal muscle induced by tenotomy,” Neurology, 8, No. 6, 737–740 (1968).
R. Roy, K. Baldwin, and V. R. Edgerton, “The plasticity of skeletal muscle: effects of neuromuscular activity,” Exerc. Sport Sci. Rev., 19, No. 2, 269–312 (1991).
S. Salmons and G. Vrbova, “The effect of tenotomy on the structure of fast and slow muscles of the rabbit,” Quart. J. Exp. Physiol., 49, No. 4, 424–430 (1964).
S. Shafiq, M. Gorycki, S. Asiedy, and A. Milhorat, “Tenotomy: effect on the fine structure of the soleus of the rat,” Arch. Neurol., 20, No. 4, 625–633 (1969).
D. Stephenson, G. Lamb, and G. Stephenson, “Events of the excitation-contraction-relaxation (E-C-R) cycle in fast and slow twitch mammalian muscle fibres relevant to muscle fibre fatigue,” Acta Physiol. Scand., 162, No. 2, 229–245 (1998).
J. Sutko and J. Airey, “Ryanodine receptor Ca2+ release channels. Does diversity inform equal diversity in function?” Physiol. Rev., 76, No. 4, 1027–1071 (1996).
R. Tomanek and R. Copper, ”Ultrastructural changes in tenotomized fast and slow-twitch muscle fibres,” J. Anat., 113, No. 3, 409–429 (1972).
L. Tubman, B. MacIntosh, and D. Rassier, “Absence of myosin light chain phosphorylation and twitch potentiation in atrophied skeletal muscle,” Can. J. Physiol. Pharmacol., 74, No. 6, 723–728 (1991).
A. Tubman, D. Raissier, and B. MacIntosh, “Attenuation of myosin light chain phosphorylation and post-tetanic potentiation in atrophied skeletal muscle,” Pflügers Arch. Eur. J. Physiol., 434, No. 4, 848–851 (1997).
G. Vrbova, “Changes in motor reflexes produced by tenotomy,” J. Physiol. (London), 166, No. 1, 241–250 (1963).
G. Vrbova, “The effect of motoneurone activity on the speed of contraction of striated muscle,” J. Physiol. (London), 169, No. 3, 513–526 (1963).
R. Wroblewski and L. Edstrom, “Changes in elemental composition of single muscle fibres following tenotomy of the rat soleus muscle,” Muscle Nerve, 6, No. 3, 490–496 (1983).
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Translated from Rossiiskii Fiziologicheskii Zhurnal imeni I. M. Sechenova, Vol. 97, No. 8, p. 781–794, August, 2011.
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Arutyunyan, R.S., Zhabko, E.P. Effects of Chronic Tenotomy on the Post-Tetanic Responses of Fast and Slow Muscles in Rats. Neurosci Behav Physi 43, 470–478 (2013). https://doi.org/10.1007/s11055-013-9757-5
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DOI: https://doi.org/10.1007/s11055-013-9757-5