Histochemical reactions in which the incubation mixtures had different pH values were used to study the locations of NADPH-diaphorase (NADPH-D) in the spinal cord (SC) and spinal ganglia (SG) in adult rats. The results showed that use of an incubation mixture with pH 7.4 detected neurons with different NADPH-D activities and that not only the enzyme distribution density but also the number of NADPH-Dpositive neurons could be assessed. Not all SC and SG neurons were found to contain NADPH-D activity: high levels of activity were seen in small and intermediate SG neurons, while motoneurons had low levels of activity. Other cellular structures in the SC also had NADPH-D activity and were interneurons.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
I. V. Dyuizen, I. I. Deridovich, R. A. Kurbatskii, and V. V. Shorin, “NO-ergic neurons in the cervical nuclei of the rat brain in normal conditions and after administration of opiates,” Morfologiya, 123, No. 2, 24–29 (2003).
E. V. Eliseeva, N. E. Romanov, V. F. Baranov, and P. A. Motavkin, “Nitric oxide synthase in neurons in the posterior nucleus and vagus nerve ganglia and changes in response to inhalation of acetylcholine in normal conditions and experimental bronchial asthma,” Morfologiya, 123, No. 4, 32–36 (2002).
D. E. Korzhevskii and V. A. Otellin, “Distribution of nitric oxide synthase in rat cerebral cortex cells,” Morfologiya, 110, No. 6, 37–41 (1996).
D. E. Korzhevskii, V. A. Otellin, I. P. Grigoriev, et al., “Immunocytochemical detection of neuronal NO synthase in rat brain cells,” Morfologiya, 132, No. 4, 77–80 (2007).
A. E. Kotsyuba, E. P. Kotsyuba, and V. M. Chertok, “Nitroxidergic nerve fibers in intracerebral vessels,” Morfologiya, 135, No. 2, 27–32 (2009).
V. I. Lapsha, V. N. Bocharova, and V. N. Gurin, “Changes in the activity of NO synthase, the enzymes of energy metabolism, and ultrastructure in neurons in the cerebral cortex in a model of transient ischemia,” Morfologiya, 123, No. 2, 32–36 (2003).
R. Lillie, “Neurons,” in: Histopathologic Technic and Practical Histochemistry [Russian translation], Mir (1969), pp. 349–354.
N. Yu. Matveeva, S. G. Kalinichenko, I. I. Pushchin, and P. A. Motavkin, “The role of nitric oxide in the apoptosis of retinal neurons in the human fetal eye,” Morfologiya, 129, No. 1, 42–48 (2006).
P. A. Motavkin, N. A. Andreeva, and T. A. Shumatova, “Nitroxidergic neurons in the respiratory organs,” Morfologiya, 117, No. 1, 10–13 (2000).
S. N. Olenov, “The spinal cord,” in: Structure of the Brain [in Russian], Meditsina, Leningrad (1987), pp. 51–62.
V. E. Okhotin and S. G. Kalinichenko, “Localization of NO synthase in Lugaro cells and the mechanisms of NO-ergic interactions between inhibitory interneurons in the cerebellar cortex of the rabbit,” Morfologiya, 115, No. 3, 52–61 (1999).
V. E. Okhotin and V. V. Kupriyanov, “Neurovasal relationships in the neocortex of the human brain,” Morfologiya, 110, No. 4, 17–22 (1996).
V. E. Okhotin and A. V. Shuklin, “Significance of the neuronal, endothelial, and inducible isoforms of NO synthase in the histophysiology of cardiac muscle,” Morfologiya, 129, No. 1, 7–15 (2006).
E. Pierce, “Diaphorases and dehydrogenases,” in: Histochemistry [Russian translation], Izd. Inostrannoi Literatury, Moscow (1962), pp. 503–531.
S. V. Chusovitina and A. A. Varaksin, “Nitroxidergic innervation of the digestive tract of the Japanese anchovy,” Morfologiya, 123, No. 3, 50–53 (2003).
A. V. Shuklin and V. N. Shvalev, “NO synthase in intracardiac ganglia in humans in normal conditions and myocardial ischemia,” Morfologiya, 129, No. 3, 34–36 (2006).
B. T. Hope, G. J. Michael, K. M. Knigge, and S. R. Vincent, “Neuronal NADPH diaphorase is a nitric oxide synthase,” Neurobiology, 88, 2811–2814 (1991).
S. X. Ma, A. T. Molley, A. Sandra, et al., “Increased expression of nitric oxide synthase in the gracile nucleus of aged rats,” J. Neurosci., 76, No. 3, 659–663 (1997).
M. A. Vizzard, S. L. Erdman, J. R. Roppolo, et al., “Differential localization of neuronal nitric oxide synthase immunoreactivity and NADH-diaphorase activity in the cat spinal cord,” Cell. Tiss. Res., 278, No. 2, 299–309 (1994).
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Morfologiya, Vol. 137, No. 2, pp. 13–17, March–April, 2010.
Rights and permissions
About this article
Cite this article
Porseva, V.V., Shilkin, V.V. NADPH-Diaphorase-Positive Structures in the Spinal Cord and Spinal Ganglia. Neurosci Behav Physi 41, 223–227 (2011). https://doi.org/10.1007/s11055-011-9403-z
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11055-011-9403-z