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Comparative Analysis of the Responses of Orexin-Containing Neurons to Administration of Different Doses of Lipopolysaccharide

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Many studies are currently addressing the involvement of orexin-containing hypothalamic neurons in the responses of the CNS to administration of antigens, particularly lipopolysaccharide (LPS). The dynamics of changes in the number of orexin-containing neurons in hypothalamic structures in brain slices were assessed 2, 4, and 6 h after administration of LPS at a low, non-septic dose. Comparative analysis of the responses of orexin-containing neurons after administration of a non-septic and a higher, subseptic, dose of LPS was performed. LPS at a dose of 25 μg/kg was followed 2 and 4 h later by increases in the numbers of orexin-positive neurons, with subsequent decreases in numbers, while the higher LPS dose of 500 μg/kg altered the neuron count only at 6 h, with a more significant decline in the number of orexin-containing neurons. These data provide evidence for changes in the content of orexin detected within neurons and a change in the balance of orexin synthesis and utilization processes in response to administration of an antigen.

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Authors and Affiliations

  1. Research Institute of Experimental Medicine, Russian Academy of Medical Sciences, 12 Academician Pavlov Street, 197376, St. Petersburg, Russia

    S. V. Perekrest, T. V. Abramova & N. S. Novikova

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  1. S. V. Perekrest
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  2. T. V. Abramova
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  3. N. S. Novikova
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Correspondence to S. V. Perekrest.

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Translated from Rossiiskii Fiziologicheskii Zhurnal imeni I. M. Sechenova, Vol. 95, No. 12, pp. 1336–1345, December, 2009.

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Perekrest, S.V., Abramova, T.V. & Novikova, N.S. Comparative Analysis of the Responses of Orexin-Containing Neurons to Administration of Different Doses of Lipopolysaccharide. Neurosci Behav Physi 41, 206–212 (2011). https://doi.org/10.1007/s11055-011-9401-1

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