Abstract
The invasive growth, proliferation, and transcriptional regulation of glioma C6 cells treated with endothelin-1 and PD98059, a specific inhibitor of ERK1/2 were studied. The proliferation of glioma C6 cells was assessed in different growth conditions by prior in vitro treatment with endothelin-1 followed by implantation into the brain. In vitro studies showed that PD98059 inhibited the proliferation of cultured glioma C6 cells and activated transcription factors E2F1 and Myc-Max. Endothelin-1 significantly increased the proliferation of glioma C6 cells. The model used in this study was experimental and may allow the specific features of the in vitro behavior of cultured invasive cells to be identified.
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References
N. C. Andrews and D. V. Fuller, “A rapid micropreparation technique for extraction of DNA-binding proteins from limiting number of mammalian cells,” Nucl. Acids Res., 19, 24 (1991).
J. Barg, M. M. Belcheva, R. Levy, et al., “Opioids inhibit endothelin-mediated DNA synthesis, phosphoinositide turnover, and Ca2+ mobilization in rat C6 glioma cells,” J. Neurosci., 14, 5858–5864 (1994).
K. Blumer and G. Johnson, “Diversity in function and regulation of MAP kinase pathways,” Trends Biochem. Sci., 19, 236–240 (1994).
E. Cane and L. Mahadevan, “Parallel signaling processing among mammalian MAPKS,” Trends Biochem. Sci., 20, 117–122 (1995).
L. Chang and M. Karin, “Mammalian MAP kinase signaling cascades,” Nature, 410, 37–40 (2001).
P.-O. Courand, O. Durieu-Trautmann, D. L. Nguyen, et al., “Functional endothelin-1 receptors in rat astrocytoma C6,” Eur. J. Pharmacol., 206, 191–198 (1991).
N. Dyson, “The regulation of E2F by pRB-family proteins,” Genes Dev., 12, 2245–2262 (1998).
J. R. Nevins, “Toward an understanding of the functional complexity of the E2F and retinoblastoma families,” Cell Growth Differ., 9, 585–593 (1998).
J. R. Nevins, “The Rb/E2F pathway and cancer,” Hum. Mol. Genet., 10, 699–703 (2001).
A. Saito, R. Shiba, S. Kimura, et al., “Vasoconstrictor responses of large cerebral arteries of cats to endothelin, an endothelium-derived vasoactive peptide,” Eur. J. Pharmacol., 162, 353–358 (1989).
P. J. S. Stork and J. M. Schmitt, “Crosstalk between cAMP and MAP kinase signaling in the regulation of cell proliferation,” Trends Cell Biol., 12, 258–266 (2002).
Z. S. Tencheva, M. M. Belcheva, A. A. Velichkova, et al., “Opioid regulation of AP-1 DNA binding activity in NG 108-15 cells under conditions of opioid receptor adaptation,” Mol. Brain Res., 48, 156–158 (1997).
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Translated from Morfologiya, Vol. 132, No. 6, pp. 26–29, November–December, 2007.
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Tencheva, T.S., Panov, I.R. Intracerebral development of transplanted glioblastoma C6 cells in rats after preliminary exposure to neuropeptides and an MAPK inhibitor. Neurosci Behav Physi 38, 913–916 (2008). https://doi.org/10.1007/s11055-008-9072-8
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DOI: https://doi.org/10.1007/s11055-008-9072-8