Abstract
The aim of the present work was to evaluate structural and metabolic changes in histaminergic neurons in hypothalamic nucleus E2 in rats in conditions of complete external drainage of bile. Studies were performed on male Wistar rats (n = 45). Controls consisted of animals subjected to sham surgery with preservation of physiological bile flow throughout the experiment. Quantitative histological and histochemical methods were used. Serial frontal cryostat sections cut from the posterior hypothalamus were used for detection of the activity of the following enzymes: monoamine oxidase B, succinate dehydrogenase, NADH dehydrogenase, NADPH dehydrogenase, glucose-6-phosphate dehydrogenase, lactate dehydrogenase, and acid phosphatase. Morphological studies of histaminergic neurons were performed on preparations stained with thionine. These studies showed that complete external drainage of bile led to transient size reductions and rounding of cell perikarya. Metabolic changes were seen within a day of bile loss and subsequently progressed. All energy metabolic pathways were suppressed and acid phosphatase activity was increased on day 5.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
L. S. Vasilevskaya, “Drainage of a lipoprotein complex with bile in rats,” Vopr. Pitaniya, 24, No. 3, 47–49 (1965).
S. M. Gorshkova and I. T. Kurtsin, The Mechanism of Bile Production [in Russian], Nauka, Leningrad (1967).
S. V. Emel'yanchik and S. M. Zimatkin, “Biochemical changes in the bile and serum during complete external draining of bile in rats,” Zh. Grodnensk. Gos. Med. Univ., No. 4, 43–45 (2005).
S. M. Zimatkin, V. B. Kuznetsova, and O. V. Anishchik, “The histaminergic system of the brain,” Morfologiya, 123, No. 2, 97–105 (2003).
S. M. Zimatkin, V. B. Kuznetsova, and O. N. Strik, “Spatial organization and morphometric characteristics of histaminergic neurons in the rat brain,” Morfologiya, 127, No. 2, 27–30 (2005).
S. M. Zimatkin and V. F. Tsydik, “A histochemical method for estimating monoamine oxidase A and B activities in the brain,” Morfologiya, 106, No. 4, 157–161 (1994).
N. M. Kuzin, S. S. Dadvani, and P. S. Vetshev, “Laparoscopic and traditional cholecystectomy: comparison of immediate results,” Khirurgiya, No. 2, 25–27 (2000).
S. D. Podymova, Liver Diseases: A Handbook for Physicians [in Russian], Meditsina, MV (1998).
E. Pierce, Theoretical and Applied Histochemistry [Russian translation], Izdatel'stvo Inostrannoi Literatury (Foreign Literature Press), Moscow (1962).
S. Sherlock and J. Dooley, Diseases of the Liver and Biliary Tract [Russian translation], GÉOTAR Meditsina, Moscow (1999).
N. Inagaki, K. Toda, and I. Taniuchi, “The five subgroups of the tuberomammillary nuclei of the rat: an analysis of the histaminergic efferent projections to the medial preoptic area and inferior colliculus,” Exptl. Brain Res., 80, 374–380 (1990).
H. Haas and P. Panula, “The role of histamine and the tuberomamillary nucleus in the nervous system,” Nat. Rev. Neurosci., 4, 121–130 (2003).
M. Malavolti, H. Fromm, S. Ceryak, and K. L. Shehan, “Cerebral low-density lipoprotein (LDL) uptake is stimulated by acute bile drainage,” Biochem. Biophys Acta, 1081, 106–108 (1991).
G. Paxinos and C. Watson, The Rat Brain in Stereotaxic Coordinates, Academic Press, New York (1986), Second Edition.
Author information
Authors and Affiliations
Additional information
__________
Translated from Morfologiya, Vol. 132, No. 6, pp. 22–25, November–December, 2007.
Rights and permissions
About this article
Cite this article
Zimatkin, S.M., Baraban, O.V. & Emel’yanchik, S.V. Structural-metabolic changes in histaminergic neurons of the rat hypothalamus in conditions of loss of bile. Neurosci Behav Physi 38, 907–911 (2008). https://doi.org/10.1007/s11055-008-9069-3
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11055-008-9069-3