Abstract
This report presents results from studies on the actions of neutrophil defensin NP-1 on the initial stage of regeneration of the lesioned sciatic nerve in rats. The effects of defensin on the growth rate and functional characteristics of regenerating nerve fibers were assessed by recording total action potentials 21 days after transection and microsurgical suturing of the nerve. These experiments showed that defensin increased the rate of growth of regenerating nerve fibers by 30%: the distance over which nerve spike conductivity was restored in the lesioned nerve increased from 7.2 ± 1.2 (control) to 10.5 ± 0.8 mm (experiment) from the suturing site (p < 0.05). In addition, an increase in the excitation conduction rate along the regenerating nerve fibers by 20% compared with control was observed. Overall, the results provide evidence for the positive effects of defensin on restoration of the functions of the lesioned nerve trunk.
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REFERENCES
V. N. Kokryakov, The Biology of Antibiotics of Animal Origin [in Russian], Nauka, St. Petersburg (1999).
L. I. Kolosova, A. B. Moiseeva, O. V. Ryabchikova, and G. N. Akoev, “Effects of RB-101, a blocker of enkephalin-degrading enzymes, on restoration of the conductivity of the lesioned rat sciatic nerve,” Ros. Fiziol. Zh. im. I. M. Sechenova, 83, No. 11–12, 74–78 (1997).
L. I. Kolosova, A. B. Moiseeva, L. N. Turchaninova, V. V. Malinin, E. L. Polyakov, A. D. Nozdrachev, and V. Kh. Khavinson, “The delayed effect of cortagen on recovery of function in lesioned nerves,” Dokl. Ros. Akad. Nauk., 384, No. 2, 271–273 (2003).
B. A. Kudryashov, M. V. Kondashevskaya, L. A. Lyapina, V. N. Kokryakov, Yu. A. Mazing, and O. V. Shamova, “The actions of defensin on the healing process in aseptic skin wounds and blood vessel permeability,” Byull. Eksperim. Biol. Med., 59, No. 4, 391–393 (1990).
G. N. Akoev, O. B. Ilyinsky, L. I. Kolosova, M. I. Titov, and O. G. Trofimova, “The effect of opioid peptide on the functional recovery of damaged neuronal structures,” in: Mechanoreceptors. Development. Structure and Functions, Plenum Press, New York, London (1989), pp. 175–180.
N. Borregaard, P. Elsbach, T. Ganz, P. Garred, and A. Svejgaard, “Innate immunity: from plants to humans,” Immun. Today, 21, 68–70 (2000).
J. G. Boyd and T. Gordon, “Neurotrophic factors and their receptors in axonal regeneration and functional recovery after peripheral nerve injury,” Molec. Neurobiol., 27, No. 3, 277–324 (2003).
R. Chen, L. G. Cohen, and M. Hallett, “Nervous system reorganization following injury,” Neurosci., 111, No. 4, 761–773 (2003).
J. Donnerer, “Regeneration of primary sensory neurons,” Pharmacol., 67, 169–181 (2003).
M. Frye, J. Bargon, and R. Gropp, “Expression of human β-defensin-1 promotes differentiation of keratinocytes,” J. Mol. Med., 79, 275–282 (2001).
J. L. Goldberg and B. A. Barres, “The relationship between neuronal survival and regeneration,” Ann. Rev. Neurosci., 23, 579–612 (2000).
G. H. Gudmundsson and B. Agerberth, “Neutrophil antibacterial peptides, multifunctional effector molecules in the mammalian immune system,” J. Immunol. Meth., 232, 45–54 (1999).
R. E. W. Hancock and G. Diamond, “The role of cationic antimicrobial peptides in innate host defences,” Trends Microbiol., 8, No. 9, 402–410 (2000).
T. Levi-Montalcini and V. Hamberger, “Selective growth stimulating effects of mouse sarcoma on the sensory and sympathetic nervous system of the chick,” J. Exptl. Zool., 116, No. 2, 321–361 (1951).
A. Markus, T. D. Patel, and W. D. Snider, “Neurotrophic factors and axonal growth,” Curr. Opin. Neurobiol., 12, No. 5, 523–531 (2002).
S. Meiners and M. L. Mercado, “Functional peptide sequences derived from extracellular matrix glycoproteins and their receptors: strategies to improve neuronal regeneration,” Mol. Neurobiol., 27, No. 2, 177–196 (2003).
A. R. Periathamby and A. R. Dentino, “Current status of defensins and their role in innate and adaptive immunity,” FEMS Microbiol. Lett., 206, 9–18 (2002).
M. Salzet, “Antimicrobial peptides are signaling molecules,” Trends Immunol., 23, 283–284 (2002).
J. Sjoberg and M. Kanje, “The initial period of peripheral nerve regeneration and the importance of the local environment for the conditioning lesion effect,” Brain Res., 530, 167–169 (1990).
O. E. Sorensen, J. B. Cowland, K. Theilgaard-Monch, T. Ganz, and N. Borregaard, “Wound healing and expression of antimicrobial peptides in human keratinocytes, a consequence of common growth factors,” J. Immunol., 170, No. 11, 5583–5589 (2003).
S. van Wetering, S. P. G. Mannesse-Lazeroms, M. A. J. A. van Sterkenburg, and P. S. Hiemstra, “Neutrophil defensins stimulate the release of cytokines by airway epithelial cells: modulation by dexamethasone,” Inflamm. Res., 51, 8–15 (2002).
D. Young, A. Biragyn, L. W. Kwak, and J. Oppenheim, “Mammalian defensins in immunity: more than just microbicidal,” Trends Immunol., 23, No. 6, 291–296 (2002).
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Translated from Rossiiskii Fiziologicheskii Zhurnal imeni I. M. Sechenova, Vol. 91, No. 3, pp. 309–313, March, 2005.
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Nozdrachev, A.D., Kolosova, L.I., Moiseeva, A.B. et al. The Role of Defensin NP-1 in Restoring the Functions of an Injured Nerve Trunk. Neurosci Behav Physiol 36, 313–315 (2006). https://doi.org/10.1007/s11055-006-0018-8
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DOI: https://doi.org/10.1007/s11055-006-0018-8