Skip to main content

Advertisement

SpringerLink
Log in

Ischemic Preconditioning of the Rat Brain as a Method of Endothelial Protection from Ischemic/Repercussion Injury

  • Published:
Neuroscience and Behavioral Physiology Aims and scope Submit manuscript

Abstract

The studies reported here addressed the endothelium-protecting action of local and remote ischemic preconditioning of the brain in rats. Cerebral ischemia lasting 30 min was reproduced by thermocoagulation of the vertebral arteries with simultaneous clamping of the carotid arteries, the procedure being followed by reperfusion via the carotid arteries for 120 min (controls). The early and late phases of ischemic preconditioning and remote preconditioning were reproduced. Brain blood flow was recorded using high-frequency Doppler ultrasonography. The early and late phases of local ischemic preconditioning and the late phase of remote ischemic preconditioning were found to have endothelium-protecting actions apparent as improvements in the recovery of brain blood flow in the post-ischemic period in preconditioned rats, with lower levels of endothelial desquamation and cerebral edema. Blockade of nitric oxide synthesis eliminated the protective effects of both phases of preconditioning.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. I. V. Viktorov, “The role of nitric oxide and other free radicals in ischemic pathology of the brain, ” Izv. Ross. Akad. Med. Nauk., 4, 5–10 (2000).

    Google Scholar 

  2. T. D. Vlasov, “The reactivity of vessels and the parameters of thrombus formation in post-ischemic reperfusion,” Ros. Fiziol. Zh. im. I. M. Sechenova, 85, No.11, 1391–1395 (1999).

    CAS  Google Scholar 

  3. T. D. Vlasov, D. A. Smirnov, and G. M. Nutfullina, “Adaptation of the small intestine of rats to ischemia,” Ros. Fiziol. Zh. im. I. M. Sechenova, 87, No.1, 118–124 (2001).

    CAS  Google Scholar 

  4. A. V. Gurin, A. I. Molosh, and G. I. Sidorenko, “Interrupted ischemia — a unique adaptive phenomenon. Perspective of new pathways of pharmacological action,” Kardiologiya, 6, 45–52 (1997).

    Google Scholar 

  5. V. A. Kozlov, N. K. Artyushenko, O. V. Shalak, A. V. Vasil’ev, M. B. Girina, I. I. Girin, E. A. Morozova, and A. A. Monastyrenko, Doppler Ultrasonography in Assessing the Hemodynamics of Tissues in the Neck, Face, and Oral Cavity in Normal Conditions and Various Pathological States. A Handbook-Atlas [in Russian], St. Petersburg (2000).

  6. N. N. Petrishchev, E. V. Shlyakhto, T. D. Vlasov, and M. M. Galagudza, “The phenomenon of ischemic adaptation of the myocardium — pathophysiological mechanisms and possible perspectives of practical application, ” Ros. Fiziol. Zh. im. I. M. Sechenova, 87, No.5, 688–705 (2001).

    CAS  Google Scholar 

  7. N. N. Petrishchev and T. D. Vlasov, “The functional state of the endothelium in ischemia/reperfusion (literature review),” Ros. Fiziol. Zh. im. I. M. Sechenova, 86, No.2, 148–163 (2000).

    CAS  Google Scholar 

  8. N. N. Petrishchev, O. A. Berkovich, T. D. Vlasov, E. V. Volkova, E. B. Zueva, and E. V. Mozgovaya, “The diagnostic value of measurements of desquamated endothelial cells in blood,” Klin. Lab. Diagnostika, 1, 50–52 (2001).

    Google Scholar 

  9. V. B. Semenyutin, Yu. N. Zubkov, and I. P. Lomova, “Circulation, oxidative metabolism, and intravascular kinin formation in the brain in ischemia and reperfusion,” in: Proceedings of the IV International ‘Brain Ischemia’ Symposium [in Russian], St. Petersburg (1997), pp. 153–158.

  10. J. Castillo, R. Rama, and A. Davalos, “Nitric oxide-related brain damage in acute ischemic stroke, ” Stroke, 31, No.4, 852–857 (2000).

    CAS  PubMed  Google Scholar 

  11. M. Cerisoli, F. Ruggeri, G. F. Amelio, G. Giuliani, B. Bernardi, and M. Giulioni, “Experimental cerebral ‘no-reflow phenomenon’,” J. Neurosurg. Sci., 25, No.1, 7–12 (1981).

    CAS  PubMed  Google Scholar 

  12. L. W. Dobrucki, L. Kalinowski, W. Uracz, and T. Malinksi, “The protective role of nitric oxide in the brain ischemia,” J. Physiol. Pharmacol., 51, No.4, Part 1, 695–703 (2000).

    CAS  PubMed  Google Scholar 

  13. P. Golino, M. Ragni, P. Cirillo, V. E. Avvedimento, A. Feliciello, N. Esposito, A. Scognamiglio, B. Iaccarino, M. Condorelli, M. Chiariello, and G. Ambrosio, “Effects of tissue factor induced by oxygen free radicals on coronary flow during reperfusion,” Nat. Med., 2, No.1, 35–40 (1996).

    Article  CAS  PubMed  Google Scholar 

  14. T. Hayashi, Y. Itoyama, and K. Abe, “Vascular endothelial growth factor: protection against brain damage with MCA occlusion in rats,” in: Ischemic Blood Flow in the Brain, Y. Fukuuchi et al. (eds.), Springer, Tokyo (2001), pp. 120–127.

    Google Scholar 

  15. S. N. Jerome, C. W. Smith, and R. J. Korthuis, “CD18-dependent adherence reactions play an important role in the development of the no-reflow phenomenon,” Amer. J. Physiol., 264, No.2, Part 2, 479–483 (1993).

    Google Scholar 

  16. J. S. Jin, R. C. Webb, and L. G. D’Alecy, “Inhibition of vascular nitric oxide-cGMP pathway by plasma from ischemic hindlimb of rats,” Ame r. J. Physiol., 269, No.1, Part 2, 254–261 (1995).

    Google Scholar 

  17. R. A. Kloner, R. Bolli, E. Marban, L. Reinlib, and E. Braunwald, “Medical and cellular implications of stunning, hibernation, and preconditioning: an NHLBI workshop [special report],” Circulation, 97, No.18, 1848–1867 (1998).

    CAS  PubMed  Google Scholar 

  18. N. N. Petrishchev, T. D. Vlasov, V. G. Sipovsky, D. I. Kurapeev, and M. M. Galagudza, “Does nitric oxide generation contribute to the mechanism of remote ischemic preconditioning?” Pathophysiol., 7, No.4, 271–274 (2001).

    Article  CAS  Google Scholar 

  19. A. Rubino and D. M. Yellon, “Ischaemic preconditioning of the vasculature: an overlooked phenomenon for protecting the heart?” Trends Pharmacol. Sci., 21, No.6, 225–230 (2000).

    Article  CAS  PubMed  Google Scholar 

  20. A. Sola, J. D. Rosello-Catafau, V. Alfaro, J. Pesquero, L. Palacios, E. Gelpi, and G. Hotter, “ Modification of glyceraldehyde-3-phosphate dehydrogenase in response to nitric oxide in intestinal preconditioning, ” Transplantation, 67, No.11, 1446–1452 (1999).

    Article  CAS  PubMed  Google Scholar 

  21. B. J. Ward and A. McCarthy, “Endothelial cell ‘swelling’ in ischaemia and reperfusion, ” J. Mol. Cell. Cardiol., 27, No.6, 1239–1300 (1995).

    Article  Google Scholar 

  22. E. O. Weselcouch, A. J. Baird, P. Sleph, and G. J. Grover, “Inhibition of nitric oxide synthesis does not affect ischemic preconditioning in isolated perfused rat hearts,” Amer. J. Physiol., 268, No.1, Part 2, H242–H249 (1995).

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Academician Pavlov St. Petersburg State Medical University, 6/8 Lev Tolstoy Street, 197022, St. Petersburg, Russia

    T. D. Vlasov & E. A. Polyakova

  2. Institute of Experimental Medicine, Russian Academy of Medical Sciences, 12 Academician Pavlov Street, 197022, St. Petersburg, Russia

    D. E. Korzhevskii

Authors
  1. T. D. Vlasov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. E. Korzhevskii
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. A. Polyakova
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Translated from Rossiiskii Fiziologicheskii Zhurnal imeni I. M. Sechenova, Vol. 90, No. 1, pp. 40–48, January, 2004

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vlasov, T.D., Korzhevskii, D.E. & Polyakova, E.A. Ischemic Preconditioning of the Rat Brain as a Method of Endothelial Protection from Ischemic/Repercussion Injury. Neurosci Behav Physiol 35, 567–572 (2005). https://doi.org/10.1007/s11055-005-0095-0

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11055-005-0095-0

Key Words

Search

Navigation