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Phospholipid-based nano drug delivery system of curcumin using MSP1D1 protein and poloxamer 407: a comparative study for targeted drug delivery to the brain

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Abstract

This study aims to investigate a potential alternative for MSP1D1 protein to develop a lipid polymer hybrid nanoparticle (LPHN) system of curcumin using poloxamer 407 and its targeted drug delivery to the brain. Design of experiment (DoE) was used to optimize the lipid nanodisc and LPHN delivery system of curcumin by the thin film hydration method. Solid-state characterization of the optimized lipid nanodiscs and LPHNs was performed using DLS, TEM, SEM, PXRD, and DSC. In vitro release, stability study, and in vivo anti-inflammatory and bioavailability studies in mice brains were performed for the optimized LPHN delivery system. DLS and microscopic data showed that the average sizes of the lipid nanodisc and LPHN systems were 125–198 nm and 135–240 nm, respectively. The LPHN delivery system of curcumin exhibited high entrapment efficiency (95.7 ± 2.2%) compared to lipid nanodiscs (91.2 ± 1.7%). In vitro release data showed slow release of lipid nanodiscs and LPHN systems, particularly after 72 h (47.6% and 37.6%, respectively). Stability data indicated that the LPHN delivery system of curcumin was stable after 3 months, while significant growth in size was observed for lipid nanodiscs. PXRD and DSC results showed the partial amorphization of the LPHN system. In vivo anti-inflammatory data suggested that curcumin has anti-inflammatory activity when delivered as an LPHN system. In vivo bioavailability study showed curcumin (6.3 ng/mL) in brain homogenates of mice treated with the LPHN delivery system, suggesting its potential for targeting the brain to treat neurodegenerative diseases.

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Acknowledgements

The authors would like to extend their sincere appreciation to the Researchers Supporting Project Number (RSP2023R301), King Saud University, Riyadh, Saudi Arabia. We would like to thank Samia Rahman Subah and Zarin Nawal Audrika for their technical support during the research work.

Funding

The project is funded by the CTRG research grant of North South University, grant number: (CTRG-19-SHLS-10), North South University, Dhaka, Bangladesh, and Researchers Supporting Project Number (RSP2023R301), King Saud University, Riyadh, Saudi Arabia.

Author information

Authors and Affiliations

  1. Department of Pharmaceutical Sciences, North South University, Dhaka, Bangladesh

    Mohammad Hossain Shariare, Mahbia Mannan, Fairouz Khan & Mahbubur Rahman

  2. Department of Chemistry, Bangladesh University of Engineering & Technology (BUET), Dhaka, 1000, Bangladesh

    Ayesha Sharmin

  3. Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia

    Mohamed W. Attwa & A. F. M. Motiur Rahman

  4. Department of Pharmaceutical Sciences, College of Pharmacy, Mercer University, Atlanta, GA, 30341, USA

    Mohammad N. Uddin

  5. Department of Pharmaceutics, College of Pharmacy, King Saud University, POBOX-2457, Riyadh, 11451, Kingdom of Saudi Arabia

    Mohsin Kazi

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  1. Mohammad Hossain Shariare
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All authors made a significant contribution to the work reported, whether that is in the conception, study design, execution, acquisition of data, analysis and interpretation, or in all these areas; took part in drafting, revising or critically reviewing the article; gave final approval of the version to be published; have agreed on the journal to which the article has been submitted; and agree to be accountable for all aspects of the work.

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Correspondence to Mohsin Kazi.

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In vivo experiments were conducted based on the National Institute of Health Guide for the Care and Use of Laboratory Animals (NIH Publications No. 80–23; 1996, revised 1978) and guidelines developed by the institutional animal care and use committee (project identification code—AEC-09–2018, approved on 04/08/2018) at North South University, Bangladesh.

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Shariare, M.H., Mannan, M., Khan, F. et al. Phospholipid-based nano drug delivery system of curcumin using MSP1D1 protein and poloxamer 407: a comparative study for targeted drug delivery to the brain. J Nanopart Res 26, 12 (2024). https://doi.org/10.1007/s11051-023-05921-0

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