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Chitosan/tripolyphosphate nanoparticle as elastase inhibitory peptide carrier: characterization and its in vitro release study

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Abstract

This study is aimed at the preparation and evaluation of walnut meal-derived elastase inhibitory peptide loaded in chitosan-tripolyphosphate (CS-TPP) nanoparticles (NPs). It was shown that the maximum encapsulation efficiency of FFVPF could reach 94.58 ± 0.23%. TEM microphotographs, polydispersity index, and zeta-sizer reports indicated that FFVPF-loaded CS-TPP NPs were in nanometric range and were spherical, discrete, and uniform in size with PDI less than 0.3. FTIR analysis indicated that the peptides interacted with CS-TPP NPs through strong hydrogen bonds and electrostatic interactions. The CS-TPP FFVPF NPs showed better stability with heating treatment, pH treatment, or photochemical treatment. Moreover, the in vitro release profile of peptides was identified. The release rate of encapsulated FFVPF was released explosively to 77.22 ± 2.21% and gradually slowed down. These findings highlighted the prospect of CS-TPP NPs as an oral delivery system, and the application of peptides within food and pharmaceutical products.

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Data Availability

Data will be made available on request.

Abbreviations

FFVPF:

Phe-Phe-Val-Pro-Phe

CS:

Chitosan

TPP:

Tripolyphosphate

NPs:

Nanoparticles

EE:

Entrapment efficiency

LE:

Loading efficiency

RP-HPLC:

Reversed-phase high performance liquid chromatography

PDI:

Polydispersity index

FTIR:

Fourier transform infrared

XRD:

X-ray diffraction

TEM:

Transmission electron microscopy

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Acknowledgements

This work was supported by grants from National Key R&D Program of China (2019YFD1002400) and National Promotion Project of Scientific and Technological Achievements in Forestry and Grassland (2020133135).

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Authors and Affiliations

  1. Beijing Key Laboratory of Food Processing and Safety in Forestry, Department of Food Science and Engineering, College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083, Beijing, People’s Republic of China

    Yu Xiong, Le Cheng, Xiao-Yi Wang & Di-Feng Ren

  2. Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, National Demonstration Center for Experimental Chemistry Education, Northwest University, 710127, Xi’an, Shanxi, People’s Republic of China

    Ye-Hua Shen & Cong Li

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  1. Yu Xiong
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  2. Le Cheng
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  3. Xiao-Yi Wang
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  5. Cong Li
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Contributions

Yu Xiong: writing (original draft), investigation, and data curation. Le Cheng: writing (original draft) and investigation. Xiao-Yi Wang: investigation. Ye-Hua Shen: investigation. Cong Li: resources and methodology. Di-Feng Ren: project administration, writing (review and editing), and supervision.

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Correspondence to Di-Feng Ren.

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Xiong, Y., Cheng, L., Wang, XY. et al. Chitosan/tripolyphosphate nanoparticle as elastase inhibitory peptide carrier: characterization and its in vitro release study. J Nanopart Res 25, 30 (2023). https://doi.org/10.1007/s11051-023-05672-y

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