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Determination of prostate-specific antigen via the assembly of a two-dimensional nanoplatform

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Abstract

Two-dimensional platforms generate considerable interests due to diverse application. We report a “turn-on” fluorescent nanoprobe for prostate-specific antigen (PSA) detection. It is based on cobalt oxyhydroxide nanosheets (CoOOH NSs). Fluorescein amidite (abbreviated as FAM)-labeled aptamer probe (abbreviated as FAM-aptamer) has been adsorbed onto the nanosheets. Energy transfer between substrate and optical species has induced the quenching of FAM-aptamer emission. The strong affinity of FAM-aptamer to the target PSA causes the formation of a rigid aptamer structure. The integration between FAM-aptamer and CoOOH NSs has been drastically affected. The release of the aptamer probe from the nanosheet surface has been realized. The green luminescence has been recovered. The dynamic nanosensor exhibits highly sensitive performance toward PSA with a linear detection range from 0.1 to 5 ng/mL. Its detection limit is measured to be 56.1 pg/mL. Therefore, a simple and efficient sensing platform for the detection of prostate cancer can be established.

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Funding

Q. M. appreciates Guangzhou Science and Technology Plan (No. 202002030325); Natural Science Foundation of Guangdong Province, China (No. 2021A1515010324); and Science and Technology Plan of Guangdong Province (No. 2020A0505100055).

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Authors and Affiliations

  1. Key Laboratory of Theoretical Chemistry of Environment, Ministry of Education, School of Chemistry, South China Normal University, Guangzhou, 510006, China

    Junjie Chen, Xiangqian Li, Wei Zhou & Qianming Wang

  2. Guangzhou Reagent Environmental Technology Co., Ltd, Guangzhou, 510670, China

    Xiaoqi Yu

  3. Zhongshan ZhongDe Technology Co., Ltd, Huoju Development Zone, Zhongshan, 528437, Guangdong, China

    Wei Zhou

  4. School of Chemistry, Guangzhou Key Laboratory of Analytical Chemistry for Biomedicine, South China Normal University, Guangzhou, 510006, People’s Republic of China

    Qianming Wang

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  1. Junjie Chen
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  2. Xiangqian Li
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  3. Xiaoqi Yu
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  4. Wei Zhou
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Contributions

Junjie Chen: writing — original draft.

Xiangqian Li: investigation.

Xiaoqi Yu: assisted with data collection and data analysis.

Wei Zhou: validation.

Qianming Wang: supervision, writing, and reviewing.

Corresponding authors

Correspondence to Xiaoqi Yu or Qianming Wang.

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Chen, J., Li, X., Yu, X. et al. Determination of prostate-specific antigen via the assembly of a two-dimensional nanoplatform. J Nanopart Res 24, 88 (2022). https://doi.org/10.1007/s11051-022-05437-z

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