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Hyaluronic acid-coated liposome for active targeting on CD44 expressing tumors

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Abstract

Liposome coated with hyaluronic acid (HA) was fabricated for targeted delivery of Doxorubicin hydrochloride (DOX) to CD44 expressing tumors. DOX was incorporated into liposome (DOX-L) via a transmembrane pH-gradient method, which contributed to high encapsulation efficiency (97%) and drug loading (19%). HA was modified on the surface of DOX-L by simple vortex (HA-DOX-L). The average diameter of optimized DOX-L and H-DOX-L was 109.7 ± 3.1 and 117.2 ± 5.0 nm, respectively, with good uniformity and stability during 6-month storage. SAXS and TEM evidenced the corona of HA on the surface of DOX-L, which convinced the prolonged circulation of DOX. The apoptosis study demonstrated the improved efficacy of HA-DOX-L with the human colon cancer cell line HCT-116 cells in comparison to the conventional reservoirs. This improved efficacy of HA-DOX-L with HCT-116 cells should be related with the interaction between HA and CD44 receptor of HCT-116 cells.

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Funding

This study was funded by National Natural Science Foundation of China (Grant number 21573070). We thank the staff of BL19U2 beamline at National Center for Protein Science Shanghai and Shanghai Synchrotron Radiation Facility (Shanghai, People’s Republic of China) for assistance during data collection.

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Authors and Affiliations

  1. Shanghai Key Laboratory of Functional Materials Chemistry, State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, People’s Republic of China

    Zhenfen Tian & Aihua Zou

  2. State Key Laboratory of Bioreactor Engineering and Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, People’s Republic of China

    Jianwen Liu

  3. National Center for Protein Science Shanghai, Shanghai Institute of Biochemistry and Cell Biology, Shanghai, 200237, People’s Republic of China

    Na Li

  4. Helmholtz-Zentrum Geesthacht, Centre for Materials and Coastal Research, D-21502, Geesthacht, Germany

    Vasil M. Garamus

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  1. Zhenfen Tian
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  2. Jianwen Liu
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  3. Na Li
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  4. Vasil M. Garamus
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  5. Aihua Zou
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Correspondence to Aihua Zou.

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Tian, Z., Liu, J., Li, N. et al. Hyaluronic acid-coated liposome for active targeting on CD44 expressing tumors. J Nanopart Res 20, 235 (2018). https://doi.org/10.1007/s11051-018-4324-1

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  • DOI: https://doi.org/10.1007/s11051-018-4324-1

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