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Large-scale protein/antibody patterning with limiting unspecific adsorption

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Abstract

A simple synthetic route based on nanosphere lithography has been developed in order to design a large-scale nanoarray for specific control of protein anchoring. This technique based on two-dimensional (2D) colloidal crystals composed of polystyrene spheres allows the easy and inexpensive fabrication of large arrays (up to several centimeters) by reducing the cost. A silicon wafer coated with a thin adhesion layer of chromium (15 nm) and a layer of gold (50 nm) is used as a substrate. PS spheres are deposited on the gold surface using the floating-transferring technique. The PS spheres were then functionalized with PEG-biotin and the defects by self-assembly monolayer (SAM) PEG to prevent unspecific adsorption. Using epifluorescence microscopy, we show that after immersion of sample on target protein (avidin and anti-avidin) solution, the latter are specifically located on polystyrene spheres. Thus, these results are meaningful for exploration of devices based on a large-scale nanoarray of PS spheres and can be used for detection of target proteins or simply to pattern a surface with specific proteins.

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Acknowledgements

This study was funded by the European Institute of Membrane (health project (PS1-2015)). V. F. has fellowship from the UE Backis program.

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Authors and Affiliations

  1. Institut Européen des Membranes (IEM UMR5635), Université de Montpellier, CNRS, ENSCM, Place Eugène Bataillon, F-34095, Montpellier Cedex 5, France

    Viktoriia Fedorenko, Mikhael Bechelany, Jean-Marc Janot & Sebastien Balme

  2. Faculty of Physics, Experimental Physics Department, Odessa National I. I. Mechnikov University, 42, Pastera, Odessa, 65026, Ukraine

    Viktoriia Fedorenko & Valentyn Smyntyna

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  1. Viktoriia Fedorenko
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  2. Mikhael Bechelany
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  3. Jean-Marc Janot
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  4. Valentyn Smyntyna
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  5. Sebastien Balme
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Correspondence to Sebastien Balme.

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Fedorenko, V., Bechelany, M., Janot, JM. et al. Large-scale protein/antibody patterning with limiting unspecific adsorption. J Nanopart Res 19, 351 (2017). https://doi.org/10.1007/s11051-017-4053-x

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