Effects of PEGylation on biomimetic synthesis of magnetoferritin nanoparticles
Recent studies have demonstrated that ferrimagnetic magnetoferritin nanoparticles are a promising novel magnetic nanomaterial in biomedical applications, including biocatalysis, imaging, diagnostics, and tumor therapy. Here we investigated the PEGylation of human H-ferritin (HFn) proteins and the possible influence on biomimetic synthesis of magnetoferritin nanoparticles. The outer surface of HFn proteins was chemically modified with different PEG molecular weights (PEG10K and PEG20K) and different modification ratios (HFn subunit:PEG20K = 1:1, 1:2, 1:4). The PEGylated HFn proteins were used for biomimetic synthesis of ferrimagnetic magnetoferritin nanoparticles. We found that, compared with magnetoferritin using non-PEGylated HFn protein templates, the synthesized magnetoferritin using the PEGylated HFn protein templates possessed larger magnetite cores, higher magnetization and relaxivity values, and improved thermal stability. These results suggest that the PEGylation of H-ferritin may improve the biomineralization of magnetoferritin nanoparticles and enhance their biomedical applications.
KeywordsFerritin PEGylation Biomimetic synthesis Magnetic nanoparticles Biomedical applications
We thank colleagues Naigui Liu at NMR sub-laboratory for help in the relaxivity experiment, Xu Tang and Lixin Gu at Transmission Electron Microscopy Laboratory for their help in TEM experiments, and Greig A. Paterson for language editing. This work was supported by grants of the National Natural Science Foundation of China (41621004, 41330104, and 41574062) and the Frontier Science Program of Chinese Academy of Sciences.
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Conflict of interest
The authors declare that they have no conflict of interest.
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