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Lysozyme complexes with thermo- and pH-responsive PNIPAM-b-PAA block copolymer

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Abstract

Lysozyme is an enzyme responsible for the damage of bacterial cell walls and is abundant in a number of secretions such as tears and human milk. In the present study, we investigated the structure, the physicochemical characteristics, and the temperature-responsiveness of lysozyme complexes with poly(N-isopropylacrylamide)-b-poly(acrylic acid) block polyelectrolyte in aqueous media. A gamut of light-scattering techniques and fluorescence spectroscopy were used in order to examine the complexation process, as well as the structure, solution behavior, and temperature response of the nanosized complexes. The concentration of copolymer polyelectrolyte was kept constant. The values of the scattering intensity, I 90, which is proportional to the mass of the species in solution, increased gradually as a function of C LYS, providing proof of the occurring complexation, while the size of the nanostructures decreased. The structure of the complexes became more open as the C LYS increased. The increase of the salinity did not affect the structural characteristics of the supramolecular nanoparticulate aggregates. On the other hand, the physicochemical and structural characteristics of the complexes changed upon increasing temperature, and the changes depended on the initial ratio block polyelectrolyte/lysozyme. The knowledge on developing block polyelectrolyte/protein complexes through electrostatic interactions, obtained from this investigation, may be applied to the design of nutraceuticals.

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Acknowledgements

Authors acknowledge financial support of the work by the NANOMACRO 1129 project which is implemented in the framework of the Operational Program “Education and Life-long Learning” (Action “ARISTEIA I”) and it is co-funded by the European Union (European Social Fund) and by national funds. The authors would like to thank Dr. Aggeliki Siamidi for her help in editing this manuscript.

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Authors and Affiliations

  1. Department of Pharmaceutical Technology, Faculty of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece

    Natassa Pippa & Costas Demetzos

  2. Theoretical and Physical Chemistry Institute, National Hellenic Research Foundation, Athens, Greece

    Natassa Pippa, Anastasia Meristoudi & Stergios Pispas

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  1. Natassa Pippa
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  2. Anastasia Meristoudi
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  3. Stergios Pispas
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  4. Costas Demetzos
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Correspondence to Stergios Pispas.

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Pippa, N., Meristoudi, A., Pispas, S. et al. Lysozyme complexes with thermo- and pH-responsive PNIPAM-b-PAA block copolymer. J Nanopart Res 19, 76 (2017). https://doi.org/10.1007/s11051-017-3782-1

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  • DOI: https://doi.org/10.1007/s11051-017-3782-1

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