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Silica-coated manganite and Mn-based ferrite nanoparticles: a comparative study focused on cytotoxicity

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Abstract

Magnetic oxide nanoparticles provide a fascinating tool for biological research and medicine, serving as contrast agents, magnetic carriers, and core materials of theranostic systems. Although the applications rely mostly on iron oxides, more complex oxides such as perovskite manganites may provide a much better magnetic performance. To assess the risk of their potential use, in vitro toxicity of manganite nanoparticles was thoroughly analysed and compared with another prospective system of Mn–Zn ferrite nanoparticles. Magnetic nanoparticles of La0.63Sr0.37MnO3 manganite were prepared by two distinct methods, namely the molten salt synthesis and the traditional sol–gel route, whereas nanoparticles of Mn0.61Zn0.42Fe1.97O4 ferrite, selected as a comparative material, were synthesized by a new procedure under hydrothermal conditions. Magnetic cores were coated with silica and, moreover, several samples of manganite nanoparticles with different thicknesses of silica shell were prepared. The size-fractionated and purified products were analysed using transmission electron microscopy, dynamic light scattering, measurement of the zeta-potential dependence on pH, IR spectroscopy, and SQUID magnetometry. The silica-coated products with accurately determined concentration by atomic absorption spectroscopy were subjected to a robust evaluation of their cytotoxicity by four different methods, including detailed analysis of the concentration dependence of toxicity, analysis of apoptosis, and experiments on three different cell lines. The results, comparing two manganese-containing systems, clearly indicated superior properties of the Mn–Zn ferrite, whose silica-coated nanoparticles show very limited toxic effects and thus constitute a promising material for bioapplications.

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Acknowledgments

The study was supported by the Grant Agency of the Czech Republic through the project 15-10088S. The evaluation of the size distribution of particles by means of TEM was supported by the project P302/12/G157 from the same agency. Further, we would like to thank also to our colleagues Dr. Karel Závěta and Dr. Zdeněk Jirák for careful reading and valuable comments.

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Authors and Affiliations

  1. Institute of Physics, AS CR, Cukrovarnická 10, 162 00, Praha 6, Czech Republic

    Ondřej Kaman, Tereza Dědourková, Jakub Koktan, Jarmila Kuličková, Miroslav Maryško & Pavel Veverka

  2. Department of Inorganic Technology, Faculty of Chemical Technology, University of Pardubice, Doubravice 41, 532 10, Pardubice, Czech Republic

    Tereza Dědourková & Ladislav Svoboda

  3. Department of Analytical Chemistry, Faculty of Chemical Engineering, University of Chemistry and Technology, Prague, Technická 5, 166 28, Praha 6, Czech Republic

    Jakub Koktan

  4. Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10, Pardubice, Czech Republic

    Radim Havelek & Karel Královec

  5. Institute of Experimental Medicine, AS CR, Vídeňská 1083, 142 20, Praha 4, Czech Republic

    Karolína Turnovcová & Pavla Jendelová

  6. First Faculty of Medicine, Institute of Cellular Biology and Pathology, Charles University in Prague, Albertov 4, 128 01, Praha 2, Czech Republic

    Adam Schröfel

  7. Faculty of Science, Charles University in Prague, Viničná 7, 128 43, Praha 2, Czech Republic

    Adam Schröfel

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  1. Ondřej Kaman
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  2. Tereza Dědourková
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Correspondence to Ondřej Kaman.

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Kaman, O., Dědourková, T., Koktan, J. et al. Silica-coated manganite and Mn-based ferrite nanoparticles: a comparative study focused on cytotoxicity. J Nanopart Res 18, 100 (2016). https://doi.org/10.1007/s11051-016-3402-5

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