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Characterization of lead nanoparticles formed by Shewanella sp. KR-12

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Abstract

The bacterial strain KR-12 was isolated from river sediment in northeast Taiwan. 16S rRNA gene sequencing revealed that it belongs to the genus Shewanella. The strain can accumulate lead (Pb) and form Pb nanoparticles (PbNPs) on exposure to Pb(NO3)2 and sodium formate in HEPES buffer. On transmission electron microscopy (TEM), the KR-12-formed PbNPs were spherical in shape and ranged from 3 to 8 nm. The PbNPs formed a line or curved pattern on bacteria. In addition, one or more pilus-like structures elongated from the bacteria. In contrast, Shewanella oneidensis MR-1 and other bacteria could not form PbNPs pattern or pilus-like structure under the same conditions. High-resolution TEM combined with energy-dispersive X-ray spectroscopy demonstrated that these PbNPs primarily contained Pb and had an amorphous structure. This is the first report of the biosynthesis of PbNPs by a Shewanella species.

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Acknowledgments

The authors are grateful to the Electron Microscopy Laboratory, Veterinary Medicine of National Taiwan University for the TEM analysis and financial support from the Ministry of Science and Technology, Executive Yuan, Taiwan (grant no. NSC 101-2313-B-002-013 and NSC 102-2313-B-002-044).

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  1. Department of Agricultural Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617, Taiwan

    Chien-Liang Liu & Jui-Hung Yen

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  1. Chien-Liang Liu
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  2. Jui-Hung Yen
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Correspondence to Jui-Hung Yen.

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Liu, CL., Yen, JH. Characterization of lead nanoparticles formed by Shewanella sp. KR-12. J Nanopart Res 18, 30 (2016). https://doi.org/10.1007/s11051-015-3309-6

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