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Olive-oil nanocapsules stabilized by HSA: influence of processing variables on particle properties

  • J. A. Molina-Bolívar
  • F. Galisteo-González
Research Paper

Abstract

Liquid lipid nanocapsules (LLN) are considered to be promising drug carriers in the medical field. The size and the surface charge of these nanocarriers are of major importance, affecting their bioavailability and the in vivo behaviour after intravenous injection. This research provides a comprehensive study on the preparation of olive-oil nanocapsules stabilized with a human serum albumin shell (HSA). LLN were prepared by modified solvent-displacement method. Numerous experimental variables were examined in order to characterize their impact on LLN size, distribution, and electrophoretic mobility. Physicochemical parameters of LLN were controlled by adjusting the nanodroplet stabilizing shell of adsorbed protein molecules, which was affected by the oil:HSA ratio, pH, and ionic strength of aqueous medium. The stronger the repulsion between adsorbed HSA molecules, the smaller and more monodisperse the particles proved. Other process parameters, including the ethanol:acetone ratio, organic:aqueous phase ratio, speed of organic-phase injection, and stirring rate were examined to achieve optimum preparation conditions. LLN produced by our standardized formulation were in the range of 170–175 nm with low polydispersity index (<0.1). Long-term colloidal stability of samples was evaluated after 6 months of storage. Efficient incorporation of curcumin, a model for a water-insoluble drug, into olive-oil nanocapsules was achieved (90 %). Encapsulation of curcumin into LLN had a stabilizing effect with respect to drug photodecomposition compared to that of the free molecule in solution.

Keywords

Olive-oil nanocapsules Physicochemical parameters Curcumin Drug delivery 

Notes

Acknowledgments

We thank David Nesbitt for reviewing the English in the manuscript.

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.Department of Applied Physics II, Engineering SchoolUniversity of MálagaMálagaSpain
  2. 2.Department of Applied PhysicsUniversity of GranadaGranadaSpain

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