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Low cytotoxicity fluorescent PAMAM dendrimer as gene carriers for monitoring the delivery of siRNA

Abstract

Visual detection of gene vectors has attracted a great deal of attention due to the application of these vectors in monitoring and evaluating the effect of gene carriers in living cells. A non-viral vector, the fluorescent PAMAM dendrimer (F-PAMAM), was synthesized through conjugation of PAMAM dendrimers and fluorescein. In vitro and ex vivo experiments show that F-PAMAM exhibits superphotostability, low cytotoxicity and facilitates endocytosis by A549 cells. The vector has a high siRNA binding affinity and it increases the efficiency of cy5-siRNA delivery in A549 cells, in comparison with a cy5-siRNA monomer. Our results provide a new method for simultaneously monitoring the delivery of siRNA and its non-viral carriers in living cells.

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Acknowledgments

This project was supported by the National Natural Science Foundation of China (No. 91227122, 21375133, 31300697, 31570856, and 31571020) and Institute of Chemistry, Chinese Academy of Sciences (CMS-PY-201302).

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Correspondence to Libo Du.

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The authors declare no conflicts of interest. The authors alone are responsible for the content and writing of the paper. None of the authors has a financial conflict of interest related to this research.

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Lingmei Guan, Saipeng Huang, Zhao Chen contributed equally to this work.

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Guan, L., Huang, S., Chen, Z. et al. Low cytotoxicity fluorescent PAMAM dendrimer as gene carriers for monitoring the delivery of siRNA. J Nanopart Res 17, 385 (2015). https://doi.org/10.1007/s11051-015-3185-0

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  • DOI: https://doi.org/10.1007/s11051-015-3185-0

Keywords

  • Gene carriers
  • Nanoparticles
  • PAMAM
  • siRNA delivery
  • Fluorescence
  • Drug delivery