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The euglobulin clot lysis time to assess the impact of nanoparticles on fibrinolysis

Journal of Nanoparticle Research volume 17, Article number: 317 (2015) Cite this article

Abstract

Nanoparticles (NPs) are developed for many applications in various fields, including nanomedicine. The NPs used in nanomedicine may disturb homeostasis in blood. Secondary hemostasis (blood coagulation) and fibrinolysis are complex physiological processes regulated by activators and inhibitors. An imbalance of this system can either lead to the development of hemorrhages or thrombosis. No data are currently available on the impact of NPs on fibrinolysis. The objectives of this study are (1) to select a screening test to study ex vivo the impact of NPs on fibrinolysis and (2) to test NPs with different physicochemical properties. Euglobulin clot lysis time test was selected to screen the impact of some NPs on fibrinolysis using normal pooled plasma. A dose-dependent decrease in the lysis time was observed with silicon dioxide and silver NPs without disturbing the fibrin network. Carbon black, silicon carbide, and copper oxide did not affect the lysis time at the tested concentrations.

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Acknowledgments

The research leading to these results has received funding from the European Community’s Seventh Framework Programme (FP7/2007–2013) under Grant Agreement No. 263147 (NanoValid - Development of reference methods for hazard identification, risk assessment and LCA of engineered nanomaterials).

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Authors and Affiliations

  1. Department of Pharmacy, Namur Thrombosis and Hemostasis Center (NTHC), Namur Nanosafety Center (NNC), NAmur Research Institute for Life Sciences (NARILIS), University of Namur – UNamur, 61 Rue de Bruxelles, 5000, Namur, Belgium

    Valentine Minet, Lutfiye Alpan, François Mullier, Jean-Michel Dogné & Julie Laloy

  2. Hematology Laboratory, CHU UCL Mont-Godinne - Dinant, Yvoir, Belgium

    François Mullier

  3. Laboratory of Cellular Biochemistry and Biology (URBC), Namur, Belgium

    Olivier Toussaint

  4. Research Centre for the Physics of Matter and Radiation (PMR-LARN), Namur Nanosafety Center (NNC), NAmur Research Institute for Life Sciences (NARILIS), University of Namur (UNamur), Namur, Belgium

    Stéphane Lucas

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  1. Valentine Minet
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  2. Lutfiye Alpan
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  3. François Mullier
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  4. Olivier Toussaint
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  5. Stéphane Lucas
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  6. Jean-Michel Dogné
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  7. Julie Laloy
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Correspondence to Valentine Minet.

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Minet, V., Alpan, L., Mullier, F. et al. The euglobulin clot lysis time to assess the impact of nanoparticles on fibrinolysis. J Nanopart Res 17, 317 (2015). https://doi.org/10.1007/s11051-015-3110-6

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  • DOI: https://doi.org/10.1007/s11051-015-3110-6

Keywords

  • Nanoparticles
  • Fibrinolysis
  • Hemocompatibility
  • Blood
  • Nanomedicine