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Synthesis of magnetic thermosensitive microcontainers for enzyme immobilization

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Abstract

We present a new approach for the fabrication of magnetic thermoresponsive polymer microcapsules with mobile magnetic spherical cores. The microcontainers form fried-egg-like structures with a polymer shell layer of 50 nm due to the existence of hollow cavities. The microcontainers undergo a temperature-induced volume phase transition upon changing the temperature and present an impressive magnetic response. The magnetic saturation of these smart microcontainers (42 emu/g) is high enough to meet most requirements of bioapplications. To further investigate the potential application of these smart microcontainers in biotechnology, Candida rugosa lipase was selected for the enzyme immobilization process. The immobilized lipase exhibited excellent thermal stability and reusability in comparison with the free enzyme. The adsorption/release of the lipase from the microcontainers can be controlled by the environmental temperature and magnetic force, thus, offering new potential applications such as in controlled drug delivery, bioseparation, and catalysis.

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Acknowledgments

The authors thank the financial supports from the National Natural Science Foundation of China (Nos. 21374045, 21301081), the scientific research ability training of under-graduate students majoring in chemistry by the two patterns based on the tutorial system and top students (J1103307) and the Opening Foundation of State Key Laboratory of Applied Organic Chemistry (SKLAOC-2009-35).

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Authors and Affiliations

  1. State Key Laboratory of Applied Organic Chemistry, Institute of Biochemical Engineering & Environmental Technology, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China

    Jianzhi Wang, Guanghui Zhao, Xinyu Wang, Xiaomen Peng & Yanfeng Li

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  1. Jianzhi Wang
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  2. Guanghui Zhao
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  3. Xinyu Wang
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  4. Xiaomen Peng
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  5. Yanfeng Li
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Corresponding authors

Correspondence to Guanghui Zhao or Yanfeng Li.

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Wang, J., Zhao, G., Wang, X. et al. Synthesis of magnetic thermosensitive microcontainers for enzyme immobilization. J Nanopart Res 17, 224 (2015). https://doi.org/10.1007/s11051-015-2986-5

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  • DOI: https://doi.org/10.1007/s11051-015-2986-5

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