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High-yield aqueous synthesis of multi-branched iron oxide core–gold shell nanoparticles: SERS substrate for immobilization and magnetic separation of bacteria

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Abstract

The high product yield of multi-branched core–shell Fe3−x O4@Au magnetic nanoparticles was synthesized used as magnetic separation platform and surface-enhanced Raman scattering (SERS) substrates. The multi-branched magnetic nanoparticles were prepared by a seed-mediated growth approach using magnetic gold nanospheres as the seeds and subsequent reduction of metal salt with ascorbic acid in the presence of a stabilizing agent chitosan biopolymer and silver ions. The anisotropic growth of nanoparticles was observed in the presence of chitosan polymer matrix resulting in multi-branched nanoparticles with a diameter over 100 nm, and silver ions also play a crucial role on the growth of multi-branched nanoparticles. We propose the mechanism of the formation of multi-branched nanoparticles while the properties of nanoparticles embedded in chitosan matrix are discussed. The surface morphology of nanoparticles was characterized with transmission electron microscopy, scanning electron microscopy, ultraviolet visible spectroscopy (UV–Vis), X-ray diffraction, and fourier transform infrared spectroscopy and 57Fe Mössbauer spectrometry. Additionally, the magnetic properties of the nanoparticles were also examined. We also demonstrated that the synthesized Fe3−x O4@Au multi-branched nanoparticle is capable of targeted separation of pathogens from matrix and sensing as SERS substrates.

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Acknowledgements

This work was supported by the Scientific and Technological Research Council (TUBITAK) grant numbers 108T794 and 111T983. We also acknowledge support from Gazi BAP, project number 05/2004-03 and 05/2011-69. We would like to thank Gokhan Demirel and Mehmet Yılmaz for 3D simulation. We also thank to Dr Saime Sebnem Çetin and Prof.Dr. Suleyman Ozcelik for XRD measurements.

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Pharmacy, Gazi University, 06330, Ankara, Turkey

    Ugur Tamer & Aykut Onay

  2. Department of Chemistry and Chemical Processing Technologies, Kırıkkale Vocational High School, Kırıkkale University, Yahşihan, 71450, Kırıkkale, Turkey

    Hakan Ciftci

  3. Department of Food Engineering, Faculty of Engineering, Hacettepe University, Beytepe, 06800, Ankara, Turkey

    Akif Göktuğ Bozkurt

  4. Department of Food Engineering, Faculty of Engineering and Food Research Center, Hacettepe University, Beytepe, 06800, Ankara, Turkey

    İsmail Hakkı Boyacı

  5. Science Teaching Programme, Gazi Faculty of Education, Gazi University, 06500, Ankara, Turkey

    Demet Cetin

  6. Department of Biology, Faculty of Science, Gazi University, 06500, Ankara, Turkey

    Zekiye Suludere

  7. Institut des Molécules et des Matériaux du Mans – IMMM – UMR CNRS 6283, Université du Maine, Av. O. Messiaen, 72085, Le Mans Cedex 9, France

    Philippe Daniel, Fabienne Lagarde, Nader Yaacoub & Jean-Marc Greneche

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  1. Ugur Tamer
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Correspondence to Ugur Tamer.

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Tamer, U., Onay, A., Ciftci, H. et al. High-yield aqueous synthesis of multi-branched iron oxide core–gold shell nanoparticles: SERS substrate for immobilization and magnetic separation of bacteria. J Nanopart Res 16, 2624 (2014). https://doi.org/10.1007/s11051-014-2624-7

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