Investigating the effects of particle size and chemical structure on cytotoxicity and bacteriostatic potential of nano hydroxyapatite/chitosan/silica and nano hydroxyapatite/chitosan/silver; as antibacterial bone substitutes
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The restoration of defective bone tissue and complications related to surgery and fracture site infection are major concerns in orthopedic surgeries. However, it is crucial to develop osteoconductive and bacteriostatic composites. Chitosan/nano hydroxyapatite (CT/n-HAp) powder containing of Ag and Si were prepared by an in situ hybridization method. The aim of this work was to elucidate the effect of size, surface roughness, and chemical structure of mentioned nanocomposites on cytotoxicity and bacteriostatic activity via human osteoblast cells and Escherichia Coli, respectively. Particle size, surface roughness, reactive oxygen specious production, and bioactivity of nanocomposites were investigated by X ray diffraction, atomic force microscopy, DPPH assay, and SEM/UV–Visible spectrophotometer, respectively. Bacterial colony counting test, MTT assay and lactate dehydrogenase (LDH) release were performed as bacteriostatic and biocompatibility tests. The results showed that CT/n-HAp/Ag with smaller particle size in the range of 1–22.6 nm (10.00 ± 0.09 nm) than CT/n-HAp/Si in the range of 3–72.5 nm (18.00 ± 0.14 nm) exhibits higher cell viability and bacteriostatic activity, and less LDH release from cell plasma membrane. Integration of Ag into the nanocomposite hindered the release of Ag+ ions and restricts cytotoxic potential on cells. Higher cytotoxic effect of CT/n-HAp/Si might be related to proton concentration derived from nanocomposite and its chemical structure. In conclusion, the strong bone regeneration potential of CT/n-HAp and good biocompatibility and bacteriostatic activity of CT/n-HAp/Ag make it as potential bacteriostatic bone filler in site of infected bone fracture.
KeywordsNano hydroxyapatite Tri-phasic nanocomposite Particle size Silver Bacteriostatic Biocompatibility Nanomedicine
This work was supported by grant from Student’s Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran.
- Carlisle EM (1980a) A silicon requirement for normal skull formation in chicks. J Nutr 110:352–359Google Scholar
- Carlisle EM (1980b) Biochemical and morphological changes associated with long bone abnormalities in silicon deficiency. J Nutr 110:1046–1056Google Scholar
- Giovanardi D (2014) Cranial osteomyelitis due to E. coli infection in commercial layers. Vet Rec 18;174(3):76Google Scholar
- Hui Q, Chen Z, Zhiquan A, Yao J, Yaochao Z, Jiaxin W, Xin L, Bing H, Xianlong Z, Yang W (2014) Silver nanoparticles promote osteogenic differentiation of human urine-derived stem cells at noncytotoxic concentrations. Int J Nanomed 9:2469–2478Google Scholar
- Khanna R, Katti KS, Katti DR (2010) In situ swelling behavior of chitosan–polygalacturonic acid/hydroxyapatite nanocomposites in cell culture media. Int J Polym Sci 1–12Google Scholar
- Murugan R, Panduranga Rao K (2002) Biodegradable coralline hydroxyapatite composite-gel using natural alginate. Key Eng Mater 240:407–410Google Scholar
- Saravanan S, Nethala S, Pattnaik S, Tripathi A, Moorthi A, Selvamurugan N (2011) Preparation, characterization and antimicrobial activity of a bio-composite scaffold containing chitosan/nano-hydroxyapatite/nano-silver for bone tissue engineering. Int J Biol Macromol 49(2):188–193CrossRefGoogle Scholar