Biocompatible hyaluronic acid polymer-coated quantum dots for CD44+ cancer cell-targeted imaging
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The cysteamine-modified hyaluronic acid (HA) polymer was employed to coat quantum dots (QDs) through a convenient one-step reverse micelle method, with the final QDs hydrodynamic size of around 22.6 nm. The HA coating renders the QDs with very good stability in PBS for more than 140 days and resistant to large pH range of 2–12. Besides, the HA-coated QDs also show excellent fluorescence stability in BSA-containing cell culture medium. In addition, the cell culture assay indicates no significant cytotoxicity for MD-MB-231 breast cancer cells, and its targeting ability to cancer receptor CD44 has been demonstrated on two breast cancer cell lines. The targeting mechanism was further proved by the HA competition experiment. This work has established a new approach to help solve the stability and toxicity problems of QDs, and moreover render the QDs cancer targeting property. The current results indicate that the HA polymer-coated QDs hold the potential application for both in vitro and in vivo cancer imaging researches.
KeywordsQuantum dots Hyaluronic acid Biocompatibility CD44 receptor Molecular imaging Nanomedicine
We acknowledge the financial support by the Wallace H. Coulter GT/Emory-PKU BME collaborative research seed grant program and the National Key Instrumentation Development Project (2011YQ030114), as well as Guangdong Innovative Research Team Program (No. 2011S090). We also thank Dr. Andrew M. Smith for kindly providing the QDs.
- Gold MH (2007) Use of hyaluronic acid fillers for the treatment of the aging face. Clin Interv Aging 2:369Google Scholar
- Hosseinkhani H, He W-J, Chiang C-H, Hong P-D, Yu D-S, Domb A, Ou K-L (2013) Biodegradable nanoparticles for gene therapy technology. J Nanopart Res 15:1–15Google Scholar
- Kerscher M, Bayrhammer J, Reuther T (2008) Rejuvenating influence of a stabilized hyaluronic acid-based gel of nonanimal origin on facial skin aging. Dermatol Surg 34:720–726Google Scholar
- Kim KS, Kim S, Beack S, Yang JA, Yun SH, Hahn SK (2012) In vivo real time confocal microscopy for target specific delivery of hyaluronic acid-quantum dot conjugates. nanomedicine: nanotechnology. Biol Med 8:1070–1073Google Scholar
- Ying JY, Zarur A (2002) Synthesis of nanometer-sized particles by reverse micelle mediated techniques. US Patent 6,413,489Google Scholar