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Journal of Nanoparticle Research

, 16:2591 | Cite as

Nanomaterial induction of oxidative stress in lung epithelial cells and macrophages

  • Lin Wang
  • Anoop K. Pal
  • Jacqueline A. Isaacs
  • Dhimiter Bello
  • Rebecca L. CarrierEmail author
Research Paper

Abstract

Oxidative stress in the lung epithelial A549 cells and macrophages J774A.1 due to contact with commercially important nanomaterials [i.e., nano-silver (nAg), nano-alumina (nAl2O3), single-wall carbon nanotubes (CNT), and nano-titanium oxide anatase (nTiO2)] was evaluated. Nanomaterial-induced intracellular oxidative stress was analyzed by both H2DCFDA fluorescein probe and GSH depletion, extracellular oxidative stress was assessed by H2HFF fluorescein probes, and the secretion of chemokine IL-8 by A549 cells due to elevation of cellular oxidative stress was also monitored, in order to provide a comprehensive in vitro study on nanomaterial-induced oxidative stress in lung. In addition, results from this study were also compared with an acellular “ferric reducing ability of serum” (FRAS) assay and a prokaryotic cell-based assay in evaluating oxidative damage caused by the same set of nanomaterials, for comparison purposes. In general, it was found that nanomaterial-induced oxidative stress is highly cell-type dependent. In A549 lung epithelial cells, nAg appeared to induce highest level of oxidative stress and cell death followed by CNT, nTiO2, and nAl2O3. Different biological oxidative damage (BOD) assays’ (i.e., H2DCFA, GSH, and IL-8 release) results generally agreed with each other, and the same trends of nanomaterial-induced BOD were also observed in acellular FRAS and prokaryotic E. coli K12-based assay. In macrophage J774A.1 cells, nAl2O3 and nTiO2 appeared to induce highest levels of oxidative stress. These results suggest that epithelial and macrophage cell models may provide complimentary information when conducting cell-based assays to evaluate nanomaterial-induced oxidative damage in lung.

Keywords

Nanoparticles Oxidative stress Lung epithelial cell Macrophage FRAS Environmental and health effects 

Notes

Acknowledgments

The study was funded through the National Science Foundation as a Nanoscale Science and Engineering Centers Program (Award # NSF-0425826) and EEC-0425826 (Supplement). Some experiments were conducted at the George J. Kostas Nanoscale Technology and Manufacturing Research Center at Northeastern University.

Conflict of interest

None.

References

  1. Arrick BA, Nathan CF (1984) Glutathione metabolism as a determinant of therapeutic efficacy: a review. Cancer Res 44(10):4224–4232Google Scholar
  2. Baggiolini M, Walz A, Kunkel SL (1989) Neutrophil-activating peptide-1/interleukin 8, a novel cytokine that activates neutrophils. J Clin Invest 84(4):1045–1049CrossRefGoogle Scholar
  3. Bello D, Hsieh S-F, Schmidt D, Rogers E (2009) Nanomaterials properties vs. biological oxidative damage: implications for toxicity screening and exposure assessment. Nanotoxicology 3(3):249–261CrossRefGoogle Scholar
  4. Cohen J, DeLoid G, Pyrgiotakis G, Demokritou P (2012) Interactions of engineered nanomaterials in physiological media and implications for in vitro dosimetry. Nanotoxicology 7(4):417–431CrossRefGoogle Scholar
  5. Gou N, Onnis-Hayden A, Gu AZ (2010) Mechanistic toxicity assessment of nanomaterials by whole-cell-array stress genes expression analysis. Environ Sci Technol 44(15):5964–5970CrossRefGoogle Scholar
  6. Hardman R (2006) A toxicologic review of quantum dots: toxicity depends on physicochemical and environmental factors. Environ Health Perspect 114(2):165–172CrossRefGoogle Scholar
  7. Hsieh S-F, Bello D, Schmidt DF, Pal AK, Rogers EJ (2012) Biological oxidative damage by carbon nanotubes: fingerprint or footprint? Nanotoxicology 6(1):61–76CrossRefGoogle Scholar
  8. Koike E, Kobayashi T (2006) Chemical and biological oxidative effects of carbon black nanoparticles. Chemosphere 65(6):946–951CrossRefGoogle Scholar
  9. Lakshminarayanan V, Beno DWA, Costa RH, Roebuck KA (1997) Differential regulation of interleukin-8 and intercellular adhesion molecule-1 by H2O2 and tumor necrosis factor-α in endothelial and epithelial cells. J Biol Chem 272(52):32910–32918CrossRefGoogle Scholar
  10. Mariano M, Spector WG (1974) The formation and properties of macrophage polykaryons (inflammatory giant cells). J Pathol 113(1):1–19CrossRefGoogle Scholar
  11. Meister A, Anderson ME (1983) Glutathione. Annu Rev Biochem 52(1):711–760CrossRefGoogle Scholar
  12. Nel A, Xia T, Madler L, Li N (2006) Toxic potential of materials at the nanolevel. Science 311(5761):622–627CrossRefGoogle Scholar
  13. Rahman I, Kode A, Biswas SK (2007) Assay for quantitative determination of glutathione and glutathione disulfide levels using enzymatic recycling method. Nat Protoc 1(6):3159–3165CrossRefGoogle Scholar
  14. Rushton EK, Jiang J, Leonard SS, Eberly S, Castranova V, Biswas P, Elder A, Han X, Gelein R, Finkelstein J, Oberdörster G (2010) Concept of assessing nanoparticle hazards considering nanoparticle dosemetric and chemical/biological response metrics. J Toxic Environ Health 73(5–6):445–461CrossRefGoogle Scholar
  15. Sies H (1999) Glutathione and its role in cellular functions. Free Radic Biol Med 27(9–10):916–921CrossRefGoogle Scholar
  16. Vlahopoulos S, Boldogh I, Casola A, Brasier AR (1999) Nuclear factor-κB-dependent introduction of interleukin-8 gene epression by tumor necrosis factor: evidence for an antioxidant sensitive activating pathway distinct from nuclear translocation. Blood 94(6):1878–1889Google Scholar
  17. Wang G, Zhang J, Dewilde AH, Pal AK, Bello D, Therrien JM, Braunhut SJ, Marx KA (2012) Understanding and correcting for carbon nanotube interferences with a commercial LDH cytotoxicity assay. Toxicology 299(2–3):99–111CrossRefGoogle Scholar
  18. Xia T, Li N, Nel AE (2009) Potential health impact of nanoparticles. Annu Rev Public Health 30:137–150CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Lin Wang
    • 1
    • 2
  • Anoop K. Pal
    • 3
    • 4
  • Jacqueline A. Isaacs
    • 4
    • 5
  • Dhimiter Bello
    • 3
    • 4
  • Rebecca L. Carrier
    • 1
    Email author
  1. 1.Chemical Engineering DepartmentNortheastern UniversityBostonUSA
  2. 2.Institute for Integrated Cell-Material SciencesKyoto UniversityKyotoJapan
  3. 3.Biomedical Engineering and Biotechnology ProgramUniversity of Massachusetts LowellLowellUSA
  4. 4.Center for High Rate NanomanufacturingNortheastern UniversityBostonUSA
  5. 5.Department of Mechanical EngineeringNortheastern UniversityBostonUSA

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