Synthesis of a colloid solution of silica-coated gold nanoparticles for X-ray imaging applications
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This work proposes a method for fabricating silica-coated gold (Au) nanoparticles, surface modified with poly(ethylene glycol) (PEG) (Au/SiO2/PEG), with a particle size of 54.8 nm. X-ray imaging of a mouse is performed with the colloid solution. A colloid solution of 17.9 nm Au nanoparticles was prepared by reducing Au ions (III) with sodium citrate in water at 80 °C. The method used for silica-coating the Au nanoparticles was composed of surface-modification of the Au nanoparticles with (3-aminopropyl)-trimethoxysilane (APMS) and a sol–gel process. The sol–gel process was performed in the presence of the surface-modified Au nanoparticles using tetraethylorthosilicate, APMS, water, and sodium hydroxide, in which the formation of silica shells and the introduction of amino groups to the silica-coated particles took place simultaneously (Au/SiO2–NH2). Surface modification of the Au/SiO2–NH2 particles with PEG, or PEGylation of the particle surface, was performed by adding PEG with a functional group that reacted with an amino group in the Au/SiO2–NH2 particle colloid solution. A computed tomography (CT) value of the aqueous colloid solution of Au/SiO2/PEG particles with an actual Au concentration of 0.112 M was as high as 922 ± 12 Hounsfield units, which was higher than that of a commercial X-ray contrast agent with the same iodine concentration. Injecting the aqueous colloid solution of Au/SiO2/PEG particles into a mouse increased the light contrast of tissues. A CT value of the heart rose immediately after the injection, and this rise was confirmed for up to 6 h.
KeywordsAu Silica Core–shell Hydrogen storage Poly(ethylene glycol) X-ray contrast agent
We express our thanks to Prof. T. Noguchi at the College of Science of Ibaraki University, Japan for his support in TEM observations. This work was supported by a Grant-in-Aid for Scientific Research on Innovative Areas ‘‘Nanomedicine Molecular Science’’ (No. 2306) from Ministry of Education, Culture, Sports, Science, and Technology of Japan.
- Basile L, Passirani C, Huynh NT, Bejaud J, Benoit JP, Puglisi G, Pignatello R (2012) Serum-stable, long-circulating paclitaxel-loaded colloidal carriers decorated with a new amphiphilic PEG derivative. Int J Pharmaceut 426:231–238Google Scholar
- Hallouard F, Briancon S, Anton N, Li X, Vandamme T, Fessi H (2013) Iodinated nano-emulsions as contrast agents for preclinical X-ray imaging: Impact of the free surfactants on the pharmacokinetics. Eur J Pharm Biopharm 83:54–62Google Scholar
- Kobayashi Y, Ayame T, Nakagawa T, Kubota Y, Gonda K, Ohuchi N (2013a) Preparation of AgI/silica/poly(ethylene glycol) nanoparticle colloid solution and X-ray imaging using it. ISRN Nanomater 2013:670402Google Scholar
- Kobayashi Y, Inose H, Nagasu R, Nakagawa T, Kubota Y, Gonda K, Ohuchi N (2013c) X-ray imaging technique using colloid solution of Au/silica/poly(ethylene glycol) nanoparticles. Mater Res Innov 17:507–514Google Scholar
- Lo CL, Chou MH, Lu PL, Lo IW, Chiang YT, Hung SY, Yang CY, Lin SY, Wey SP, Lo JM, Hsiue GH (2013) The effect of PEG-5 K grafting level and particle size on tumoraccumulation and cellular uptake. Int J Pharmaceut 456:424–431Google Scholar
- Ma Y, Sadoqi M, Shao J (2012) Biodistribution of indocyanine green-loaded nanoparticles with surface modifications of PEG and folic acid. Int J Pharmaceut 436:25–31Google Scholar
- Menk RH, Schültke E, Hall C, Arfelli F, Astolfo A, Rigon L, Round A, Ataelmannan K, MacDonald SR, Juurlink BHJ (2011) Gold nanoparticle labeling of cells is a sensitive method to investigate cell distribution and migration in animal models of human disease. Nanomed Nanotechnol Biolog Med 7:647–654CrossRefGoogle Scholar
- Otsuka H, Nagasaki Y, Kataoka K (2012) PEGylated nanoparticles for biological and pharmaceutical applications. Adv Drug Deliver Rev 64:246–255Google Scholar