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Recyclable magnetic nanoparticle grafted with pH-responsive polymer for adsorption with DNA

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Abstract

Magnetite nanoparticles (MNPs) grafted with positively charged poly(2-(diethylamino) ethyl methacrylate (PDEAEMA) were prepared via a combination of atom transfer radical polymerization (ATRP) and “click” reaction and used as recyclable nanosupports for adsorption with DNA. Alkyne-terminated PDEAEMA was synthesized via ATRP and then “grafted to” azide-functionalized MNPs. Quaternized PDEAEMA-grafted MNPs were used as recyclable nanosupports for adsorption with negatively charged DNA via electrostatic interactions. The particles with the size of 3–8 nm in diameter were well dispersible in water. They responded to the change in their solution pH as observed by a consistent decrease in hydrodynamic size when solution pH changed from basic to acidic pH. Recycling efficiency of the MNPs was investigated by determining the percent adsorption of the particles after multiple cycles of the adsorption–separation–desorption process. Adsorption ability of the MNPs to T9 DNA tagged with fluorescein at 5′-position (FAM-dT9) retained higher than 80 % after 5-recycling process, indicating that these novel positively charged MNPs might be efficiently used as magnetic nanosupports for any negative biomolecules with good recycling efficiency.

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Acknowledgments

The authors thank the Thailand Research Fund (TRF) (DBG5580002) for financial funding. The Center of Excellence for Innovation in Chemistry (PERCH-CIC), Commission on Higher Education, Ministry of Education is also gratefully acknowledged for financial support. We thank Assistant Professor Dr. Pongsakorn Jantaratana from the Department of Physics, Kasetsart University, Bangkok, Thailand, for the assistance in the magnetic property measurement.

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Authors and Affiliations

  1. Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Naresuan University, Phitsanulok, 65000, Thailand

    Pawinee Theamdee, Boonjira Rutnakornpituk, Uthai Wichai & Metha Rutnakornpituk

  2. Center of Excellence in Biomaterials, Faculty of Science, Naresuan University, Phitsanulok, 65000, Thailand

    Boonjira Rutnakornpituk, Uthai Wichai & Metha Rutnakornpituk

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  1. Pawinee Theamdee
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  2. Boonjira Rutnakornpituk
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  3. Uthai Wichai
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  4. Metha Rutnakornpituk
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Correspondence to Metha Rutnakornpituk.

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Theamdee, P., Rutnakornpituk, B., Wichai, U. et al. Recyclable magnetic nanoparticle grafted with pH-responsive polymer for adsorption with DNA. J Nanopart Res 16, 2494 (2014). https://doi.org/10.1007/s11051-014-2494-z

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