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In situ functionalized fluorescent nanoparticles for efficient receptor coupling

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Abstract

An oligoethylene glycol-based propargyl phosphonate was applied to functionalize the surface of LaPO4:Ce,Tb nanoparticles in situ during the particle synthesis. The application of the surface modification reagent did not alter either size (5–7 nm in diameter) or morphology of the nanocrystalline core, but provided efficient anchor groups for subsequent coupling of a carbohydrate model receptor under mild reaction conditions. The biofunctionalization efficiency was quantified by thermogravimetric analysis and confirmed by a photometric assay. A calculation-based estimation suggested an average number of about 20 biomarkers per nanoparticles and a surface density of about 1 marker per 6 nm−2.

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Acknowledgments

Financial support for this work by the University of Malaya under research Grants RG026-09AFR, RP024-2012B, PS380-2010B, and RG264-13AFR is gratefully acknowledged.

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Authors and Affiliations

  1. Chemistry Department, Faculty of Science, University of Malaya, Lembah Pantai, 50603, Kuala Lumpur, Malaysia

    Abbas Abdulameer Salman & Thorsten Heidelberg

Authors
  1. Abbas Abdulameer Salman
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  2. Thorsten Heidelberg
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Corresponding author

Correspondence to Thorsten Heidelberg.

Electronic supplementary material

Below is the link to the electronic supplementary material.

11051_2014_2399_MOESM1_ESM.pdf

Supporting Information: Experimental details on the synthesis of surface modification reagents and the receptor model, including images of spectra to support the purity of the reagents, are provided as supplementary material. Besides, the material also includes experimental data for SAXS and the phenolic carbohydrate assay, as well as details on the calculation-based estimation of the receptor loading density on the coupled nanoparticle 11. (PDF 844 kb)

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Salman, A.A., Heidelberg, T. In situ functionalized fluorescent nanoparticles for efficient receptor coupling. J Nanopart Res 16, 2399 (2014). https://doi.org/10.1007/s11051-014-2399-x

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  • DOI: https://doi.org/10.1007/s11051-014-2399-x

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