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Targeted therapeutic effect of anti-ABCG2 antibody combined with nano silver and vincristine on mouse myeloma cancer stem cells

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Abstract

Studies from hematopoietic origin malignancies have demonstrated that multiple myeloma contain a rare population of cancer stem cells (CSCs) that are responsible for tumor multiresistance and recurrence. The goal of this study was to investigate targeted therapeutic effect of anti-ABCG2 monoclonal antibody (McAb) combined with silver nanoparticles (AgNPs) and vincristine (VCR) on myeloma CSCs. The characteristics of CD44+ CD24− cells that were isolated from the SP2/0 cells using magnetic activated cell sorting system were first identified. The results showed that the CD44+ CD24− cells exhibited higher proliferation, more colony formation, more side population fraction, and stronger tumorigenicity in BALB/c mice than the control cells. Moreover, CD44+ CD24− cells markedly up-regulated the ABCG2 expression, however, anti-ABCG2 McAb combined with AgNPs and VCR effectively inhibited the CD44+ CD24− cell growth and prolonged the survival of myeloma-bearing mice. We concluded that the CD44+ CD24− cells in mouse myeloma SP2/0 cell line posses CSC properties. Anti-ABCG2 McAb combined with AgNPs and VCR provide an efficient targeted therapeutic method for inhibiting myeloma CD44+ CD24− CSC growth in mice.

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Abbreviations

MM:

Multiple myeloma

CSCs:

Cancer stem cells

AgNPs:

Silver nanoparticles

McAb:

Monoclonal antibody

VCR:

Vincristine

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Acknowledgments

This work was supported by the 973 National Nature Science Foundation of People’s Republic of China (2011CB933500), and in part by the National Natural Science Foundation of China (No. 51201034).

Conflict of interest

The authors declare that no competing interests exist.

Author information

Authors and Affiliations

  1. Department of Pathogenic Biology and Immunology, Medical School, Southeast University, Nanjing, 210009, China

    Jun Dou, Yunjing Liu, Cuiping Yang & Dengyu Chen

  2. School of Biological Science & Medical Engineering, Southeast University, 2 Sipai Lou, Nanjing, 210096, China

    Zhihai Huang & Ning Gu

  3. Department of Medical Oncology, Affiliated Tumor Hospital of Nantong University, Nantong, 226361, China

    Xiangfeng He

  4. Department of Oncology, Zhongda Hospital, Southeast University, Nanjing, 210009, China

    Fangfang Shi

Authors
  1. Jun Dou
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  2. Xiangfeng He
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  3. Yunjing Liu
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  4. Zhihai Huang
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  5. Cuiping Yang
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  6. Fangfang Shi
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  7. Dengyu Chen
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  8. Ning Gu
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Corresponding authors

Correspondence to Jun Dou or Ning Gu.

Additional information

J. Dou, X. He and Y. Liu have contributed equally to the study.

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Dou, J., He, X., Liu, Y. et al. Targeted therapeutic effect of anti-ABCG2 antibody combined with nano silver and vincristine on mouse myeloma cancer stem cells. J Nanopart Res 15, 2127 (2013). https://doi.org/10.1007/s11051-013-2127-y

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  • DOI: https://doi.org/10.1007/s11051-013-2127-y

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