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Titanium-doped cerium oxide nanoparticles protect cells from hydrogen peroxide-induced apoptosis

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Abstract

To develop new nanoparticle materials possessing antioxidative capacity with improved physical characteristics, we have studied titanium-doped cerium oxide (CeTiO2) nanoparticles. CeTiO2 nanoparticles had mode diameters in the range of 15–20 nm. These nanoparticles demonstrated catalase activity, and did not promote the activation of hemolytic or cytolytic pathways in living cells. Using surface plasmon resonance-enhanced microscopy, we find that these nanoparticles associate with cells. Transmission electron microscopy studies demonstrated that these nanoparticles accumulate within the vacuolar compartment of cells. Importantly, CeTiO2 nanoparticles decrease hydrogen peroxide-mediated apoptosis of cells as judged by the reduced cleavage of a caspase 3-sensitive label. CeTiO2 nanoparticles may contribute to deflecting tissue damage in a broad spectrum of oxidant-mediated diseases, such as macular degeneration and Alzheimer’s disease.

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Acknowledgments

This study was supported by the Mildred E. Swanson Foundation, the Nathan Shock Center (NIH AG013283), the NIH Grant P30EY007003, and Research to Prevent Blindness.

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Authors and Affiliations

  1. Department of Ophthalmology and Visual Sciences, The University of Michigan Medical School, 1000 Wall Street, Ann Arbor, MI, 48105, USA

    Andrea Clark, Aiping Zhu & Howard R. Petty

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  1. Andrea Clark
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  2. Aiping Zhu
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  3. Howard R. Petty
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Correspondence to Howard R. Petty.

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Clark, A., Zhu, A. & Petty, H.R. Titanium-doped cerium oxide nanoparticles protect cells from hydrogen peroxide-induced apoptosis. J Nanopart Res 15, 2126 (2013). https://doi.org/10.1007/s11051-013-2126-z

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  • DOI: https://doi.org/10.1007/s11051-013-2126-z

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